Cyp11b1 (NM_001033229) Mouse Tagged ORF Clone

CAT#: MR219439

Cyp11b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 11, subfamily b, polypeptide 1 (Cyp11b1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp11b1
• Synonyms: AA389061; CPN1; Cyp11b; Cyp11b-1; FHI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219439 representing NM_001033229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGGCTCTCAGGGTGACAACAGATGTGTGGCTGGCAAGACCCTGGCAGTGCCTGCACAGGACAA
  GGGCACTGGGCACCACGGCAACACTGGCCCCAAAGACCCTGCAGCCCTTTGAAGCCATACCACAGTACTC
  CAGGAACAAGTGGCTGAAGATGATACAGATCCTGAGGGAGCAGGGCCAAGAAAACCTACACCTGGAGATG
  CATCAGGTCTTCCGGGAGCTGGGGCCCATTTTCAGGCACAGTGTAGGGAAAACACAGATTGTGTTTGTGA
  CGTTGCCGGAGGATGTTGAGAAGCTGTACCAGGTAGAGAGTACGCACCCTTGCCGCATGCCCTTGGAATC
  CTGGATAGTCCATAGAGAACTCCGTGGCCTGGGACGTGGTGTGTTCTTGCTAAATGGGCCAGAATGGTAC
  TTCAACCGACTGCAGCTGAACCCAAATGTTCTGTCACCAAAAGCCGTTCAAAAATTTGTCCCCTTGGTGG
  ATGGGATAGCAAGGGACTTTGTGGATAACCTGAAGAAGAAAATGCTGGAAAGTGTCCATGGTAGCTTCTC
  TATGGACTTTCAGTCCAGTGTGTTCAACTATACCATAGAAGCTAGCCACTTTGTTCTTTTTGGGGAGCGG
  CTGGGCCTCATTGGCCGTGATCTGAGCCCTGACAGCCTGAAGTTCCTCCACACCCTGCACTCCATGTTCA
  AAACCACCACACAGCTCTTGTACTTGCCCAGGAGCCTGACCCGATGGACAAGCACCAGGGTGTGGAAAGA
  AAACTTAGAGTCCTGGGATTTCATCTCTGAATATGTCACCAAATGTATCAAGAATGTGTATCGAGAGCTG
  GCAGAGGGTCGTCCACAGTCCTGGAGTGTCACAGCAGAGCTGGTAGCTGAGAGAACTCTGTCAATGGATG
  CCATCCAGGCTAACTCAATGGAACTCATTGCTGGAAGCACTGACACGACATCAACCCCCTTGGTAATGAC
  TTTCTTTGAGCTGGCTCGGAACCCAGATGTTCAGCAGGCCCTGAGGCAGGAGAGCCTGGCAGCTGAGGCC
  AGCATCGCTGCAAATCCTCAGAAGGCCATGTCAGACCTGCCCTTGCTGAGGGCTGCCCTTAAAGAGACCT
  TGAGGCTCTATCCTGTTGGTACTTTTCTGGAGAGAATTCTAAGCTCAGACTTGGTGCTTCAGAACTACCA
  TGTCCCTGCTGGGACAGTCCTCAATGTGAATCTGTATTCCATGGGCCGGAACCCTGCAGTGTTCCCAAGG
  CCTGAACGCTATATGCCTCAGCGCTGGCTGGAGAGGAAAAGGAGTTTCAAGCACCTGGCCTTTGGCTTTG
  GGGTGCGCCAGTGCCTGGGGAGGCGCCTGGCAGAGGCAGAGATGATGCTCCTGCTTCACCATGTGCTGAA
  ATCCTTCCACGTGGAGACACAAGAGAAAGAGGATGTGCGGATGGCCTACCGCTTCGTTTTGATGCCCAGC
  TCCAGTCCTCTCCTCACTTTCAGGCCTGTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219439 representing NM_001033229
  Red=Cloning site Green=Tags(s)
  
  MTMALRVTTDVWLARPWQCLHRTRALGTTATLAPKTLQPFEAIPQYSRNKWLKMIQILREQGQENLHLEM
  HQVFRELGPIFRHSVGKTQIVFVTLPEDVEKLYQVESTHPCRMPLESWIVHRELRGLGRGVFLLNGPEWY
  FNRLQLNPNVLSPKAVQKFVPLVDGIARDFVDNLKKKMLESVHGSFSMDFQSSVFNYTIEASHFVLFGER
  LGLIGRDLSPDSLKFLHTLHSMFKTTTQLLYLPRSLTRWTSTRVWKENLESWDFISEYVTKCIKNVYREL
  AEGRPQSWSVTAELVAERTLSMDAIQANSMELIAGSTDTTSTPLVMTFFELARNPDVQQALRQESLAAEA
  SIAANPQKAMSDLPLLRAALKETLRLYPVGTFLERILSSDLVLQNYHVPAGTVLNVNLYSMGRNPAVFPR
  PERYMPQRWLERKRSFKHLAFGFGVRQCLGRRLAEAEMMLLLHHVLKSFHVETQEKEDVRMAYRFVLMPS
  SSPLLTFRPVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033229
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033229.1, NM_001033229.2, NM_001033229.3, NP_001028401.2
• RefSeq Size: 2398 bp
• RefSeq ORF: 1506 bp
• Locus ID: 110115
• Cytogenetics: 15 34.29 cM
• MW: 58.2 kDa