Ctsa (NM_008906) Mouse Tagged ORF Clone

CAT#: MR220402

Ctsa (Myc-DDK-tagged) - Mouse cathepsin A (Ctsa), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctsa
• Synonyms: AU019505; PPCA; Ppgb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220402 representing NM_008906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCCAGTCCAAAGGCGCCTCCTGGAGAGCAAGGACGCAAGGAAGCAGAGATGCCCGGAACCGCGC
  TGTCTCCACTGCTCTTGTTGCTGCTCCTGTCCTGGGCGTCCCGGAACGAAGCAGCTCCGGACCAGGATGA
  AATCGATTGTCTCCCCGGCCTGGCCAAGCAGCCCTCTTTCCGGCAATACTCCGGCTACCTCAGAGCATCG
  GACTCCAAGCACTTCCACTACTGGTTTGTGGAGTCGCAGAACGACCCAAAGAACAGCCCCGTGGTGCTTT
  GGCTTAACGGGGGTCCCGGCTGCAGCTCGCTCGATGGGCTGCTTACAGAGCACGGCCCCTTTCTGATCCA
  GCCAGATGGTGTCACCCTGGAGTACAACCCCTATGCTTGGAACCTGATTGCCAACGTGCTGTATATCGAG
  TCCCCAGCTGGGGTGGGCTTCTCCTACTCGGATGACAAGATGTACGTGACCAATGACACAGAGGTGGCGG
  AGAACAATTATGAAGCCCTTAAAGACTTCTTCCGCCTCTTTCCGGAATACAAGGACAACAAACTTTTCCT
  GACAGGAGAGAGCTATGCTGGCATCTACATCCCCACCTTGGCTGTACTGGTCATGCAGGATCCTAGCATG
  AATCTTCAGGGGCTGGCTGTGGGCAATGGACTTGCCTCCTATGAGCAGAACGACAACTCCCTGGTCTACT
  TTGCCTACTACCATGGCCTTCTGGGGAACAGACTTTGGACTTCACTGCAGACCCACTGCTGCGCTCAGAA
  CAAGTGTAACTTCTATGACAACAAAGACCCAGAGTGTGTAAACAATCTCCTGGAAGTGTCTCGAATTGTG
  GGCAAATCTGGCCTCAACATCTACAATCTCTATGCTCCGTGTGCTGGTGGGGTGCCCGGCAGACATAGAT
  ATGAGGACACACTTGTAGTCCAGGATTTTGGCAACATCTTCACTCGCCTGCCACTTAAGCGGAGATTTCC
  TGAGGCACTGATGCGTTCTGGGGACAAGGTACGCTTGGATCCTCCCTGCACCAACACCACAGCCCCTTCC
  AACTACCTCAACAACCCCTATGTTCGGAAGGCTCTCCACATCCCCGAGTCGCTGCCCCGCTGGGACATGT
  GCAACTTCTTGGTGAATTTACAGTACCGCCGCCTCTACCAAAGCATGAACTCCCAGTACCTGAAGCTGCT
  CAGTTCACAGAAATACCAGATCCTGCTCTACAACGGAGATGTGGACATGGCCTGCAACTTCATGGGCGAT
  GAGTGGTTTGTGGATTCGCTCAACCAGAAGATGGAGGTGCAGCGCCGGCCCTGGCTAGTGGACTACGGGG
  AGAGCGGAGAACAGGTAGCTGGTTTCGTGAAGGAGTGTTCACACATCACCTTCCTCACCATCAAGGGTGC
  CGGACACATGGTCCCCACGGACAAGCCTCGAGCTGCTTTTACCATGTTCTCGAGGTTCCTGAACAAAGAG
  CCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220402 representing NM_008906
  Red=Cloning site Green=Tags(s)
  
  MTSSPKAPPGEQGRKEAEMPGTALSPLLLLLLLSWASRNEAAPDQDEIDCLPGLAKQPSFRQYSGYLRAS
  DSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIE
  SPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSM
  NLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIV
  GKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPS
  NYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGD
  EWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKE
  PY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008906
• ORF Size: 1476 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008906.3, NM_008906.4, NP_032932.2
• RefSeq Size: 3674
• RefSeq ORF: 1479
• Locus ID: 19025
• MW: 56.2 kDa
• Gene Summary: This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]