Fmo3 (NM_008030) Mouse Tagged ORF Clone

CAT#: MR220435

Fmo3 (Myc-DDK-tagged) - Mouse flavin containing monooxygenase 3 (Fmo3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fmo3
• Synonyms: AW111792
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220435 representing NM_008030.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGAAGAAAGTGGCCATCATTGGAGCTGGTGTCAGTGGCCTGGCTGCCATCAGGAGCTGTCTGGAG
  GAGGGGCTGGAGCCCACATGCTTTGAGAGGAGTGATGATGTTGGGGGCCTGTGGAAATTCTCAGACCAT
  ATAGAAGAGGGCAGGGCCAGCATTTACCAATCGGTCTTCACCAACTCTTCCAAAGAGATGATGTGTTTT
  CCAGACTTCCCCTATCCCGATGACTTTCCCAACTTCATGCATCACAGCAAGCTCCAAGAATACATCACT
  TCATTTGCCAAGGAAAAGAACCTCCTGAAATACATACAGTTTGAGACACCTGTAACCAGTATAAATAAA
  TGTCCTAATTTCTCAACTACTGGCAAATGGGAAGTCACCACTGAAAAGCACGGTAAAAAAGAAACAGCT
  GTCTTTGATGCTACAATGATTTGTTCTGGGCATCACATATTTCCCCATGTACCAAAAGACTCCTTTCCA
  GGACTGAACCGTTTTAAAGGCAAATGCTTCCACAGCAGGGACTATAAGGAACCAGGAATATGGAAGGGA
  AAACGAGTCCTGGTGATTGGCCTGGGGAACTCAGGCTGTGACATTGCTGCAGAACTCAGCCATGTAGCT
  CAGAAGGTCACCATCAGCTCTAGAAGTGGTTCTTGGGTGATGAGTCGAGTCTGGGACGATGGCTACCCT
  TGGGACATGGTGGTGCTCACACGGTTTCAAACTTTCCTCAAAAACAACTTACCCACCGCCATCTCTGAC
  TGGTGGTACACAAGGCAGATGAATGCCAGATTCAAGCACGAAAACTATGGTTTGGTGCCTTTAAACAGA
  ACACTCAGGAAAGAGCCCGTGTTCAATGATGAGCTCCCAGCCCGCATCCTGTGTGGCATGGTGACCATC
  AAGCCTAATGTAAAGGAGTTCACAGAGACGTCCGCTGTGTTTGAGGATGGGACCATGTTTGAGGCCATT
  GACTGTGTCATCTTTGCCACAGGCTATGGTTATGCCTACCCCTTCCTGGATGACTCTATTATCAAAAGC
  AGAAATAATGAGGTCACTTTGTACAAAGGTGTCTTCCCTCCTCAACTAGAGAAACCAACCATGGCAGTG
  ATTGGCCTGGTCCAGTCCCTGGGTGCCACCATCCCCATAACTGACCTGCAGGCACGCTGGGCAGCACAA
  GTAATAAAAGGAACTTGCACTTTGCCTTCTGTAAACGACATGATGGATGACATTGATGAGAAAATGGGG
  GAAAAGTTCAAATGGTATGGCAATAGCACCACCATCCAGACAGATTACATTGTTTATATGGATGAACTG
  GCCTCCTTCATTGGTGCAAAGCCCAATCTCCTATGGCTGTTTCTCAAGGATCCCAGGTTGGCTGTAGAA
  GTGTTCTTTGGCCCTTGCAGCCCCTACCAGTTCCGGCTGGTAGGCCCAGGAAAGTGGTCAGGAGCCCGG
  AACGCCATCCTAACACAGTGGGACCGATCACTGAAGCCTATGAAGACGCGTGTCGTCAGTAAAGTTCAG
  AAGTCTTGCTCTCACTTCTATTCCCGTTTGCTCAGGCTCCTGGCTGTTCCCGTTCTGCTCATTGCTTTG
  TTCCTTGTGTTGATC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR220435
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKKKVAIIGAGVSGLAAIRSCLEEGLEPTCFERSDDVGGLWKFSDHIEEGRASIYQSVFTNSSKEMMCF
  PDFPYPDDFPNFMHHSKLQEYITSFAKEKNLLKYIQFETPVTSINKCPNFSTTGKWEVTTEKHGKKETA
  VFDATMICSGHHIFPHVPKDSFPGLNRFKGKCFHSRDYKEPGIWKGKRVLVIGLGNSGCDIAAELSHVA
  QKVTISSRSGSWVMSRVWDDGYPWDMVVLTRFQTFLKNNLPTAISDWWYTRQMNARFKHENYGLVPLNR
  TLRKEPVFNDELPARILCGMVTIKPNVKEFTETSAVFEDGTMFEAIDCVIFATGYGYAYPFLDDSIIKS
  RNNEVTLYKGVFPPQLEKPTMAVIGLVQSLGATIPITDLQARWAAQVIKGTCTLPSVNDMMDDIDEKMG
  EKFKWYGNSTTIQTDYIVYMDELASFIGAKPNLLWLFLKDPRLAVEVFFGPCSPYQFRLVGPGKWSGAR
  NAILTQWDRSLKPMKTRVVSKVQKSCSHFYSRLLRLLAVPVLLIALFLVLI
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using MR220435 also available, TP520435
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008030
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008030.2, NP_032056.1
• RefSeq Size: 2020 bp
• RefSeq ORF: 1605 bp
• Locus ID: 14262
• UniProt ID: P97501
• Cytogenetics: 1 70.34 cM
• MW: 60.5 kDa
• Gene Summary: Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.[UniProtKB/Swiss-Prot Function]