Nags (NM_145829) Mouse Tagged ORF Clone

CAT#: MR220590

Nags (Myc-DDK-tagged) - Mouse N-acetylglutamate synthase (Nags), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nags
• Synonyms: 1700120E20Rik; argA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220590 representing NM_145829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGGCGTGGGTGGCCACAGCCCTGCGGTCTGCTGCAGCGGCCAGAAGGTTGCGCAGCCCGGGAG
  GTCCTGGGGGCTCACGGAGACTGAGTGGCAGCGCGCGGCGGCGCGGGGCAAAGAGCGCCAGCCCGGGACG
  CCGGCTCAGCACCGCCAGGGCTCACGCAGAGGACGCCGAGGGCGCTAAGGGTCGTGTCCAGTCTCCTGCG
  GTCGAGGAGCCGTCGTGGACACCGCTTCCAACGCCCCTCGAATCCCCTGCGCCTCCCGCCGGCAGATCAC
  TGGTGCAGCGGGACATCCAGGCCTTCCTGAACCAGTGTGGTGCCAGCCCGGGGGAAGCACGCCACTGGCT
  CACGCAATTCCAGACCTGCTACCATTCGGTGGACAAGCCCTTCGCCGTCATGGAGGTCGATGAGGAGGTG
  ATCCGGTGCCCGCAGGCGGTATCCCGCCTGGCTTTCGCCCTGGCCTTCTTGCAACGCATGGACATGAAGC
  CACTGGTGGTTCTGGGGCTGCCTACCCCCACGGCACCTTCTGGCTGTCTTTCCTTCTGGGAAGCTAAGGC
  GCAGCTTGCTCAGAGCTGCAAGGTGCTGGTGGATGAACTAAGGCACAACGCGGCTACTGCTGTGCCCTTT
  TTTGGCGGCGGATCAGTACTGAGCGCTGCGGAGCCAGCTCCCCATGCCAGCTACGGTGGCATCGTCGCGG
  TGGAGACAGACCTGTTGCAGTGGTGTCTGGAATCCAACAGCATCCCCATCCTGTGCCCCATTGGGGAAAC
  GGCTGCGCGCCGTTCCGTGCTCCTTGACTCGCTGGAGGTGACTGCATCTCTAGCCAAGGCTTTGCAGCCC
  ACCAAAATCATCTTCCTCAATAATTCAGGCGGCCTGCGGAATAACAGTCAGAAGATCCTGAGCAACGTGA
  ACCTGCCCGCTGACCTTGACCTTGTTACCAACGCTGAGTGGCTGAGCATCAAAGAGCGGCAGCAGATTCG
  GCTCATCGTGGACGTGCTGAGCCGCCTGCCGCACTACTCCTCCGCAGTCATTACAGCCGCTAGCACGCTG
  CTCACGGAACTCTTTAGTAACAAGGGCTGTGGCACCCTGTTTAAAAATGCTGAGCGGATGCTGCGAGTGC
  GCAACCTGGACAGCCTGGACCAGGGCCGCCTAGTGAACCTAGTCAACGCCAGCTTCGGCAAGAAGCTCCG
  AGAAGACTATCTGGAGTCACTGCGCCCTCGGTTGCACTCGATCTATGTCTCTGAGGGGTACAACGCGGCA
  GCCATTCTGACAGTGGAACCTGTACTAGGGGGCACCCCATATCTGGACAAATTTGTGGTGAGCTCCAGCC
  GCCAGGGCCAAGGTTCTGGACAGATGCTGTGGGAATGCCTTCGGAGAGACCTGCAAACGTTGTTCTGGCG
  CTCAAGGGTCACCAATCCCATCAATCCCTGGTACTTCAAGCACAGTGACGGTAGCTTCTCCAACAAGCAG
  TGGATCTTCTTCTGGTTCGGCCTGGCCGACATCCGAGACTCCTACGAACTAGTCAATCATGCCAAGGGGC
  TGCCGGACTCTTTCTGCAAACCAGCTTCTGACCCAGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220590 representing NM_145829
  Red=Cloning site Green=Tags(s)
  
  MATAWVATALRSAAAARRLRSPGGPGGSRRLSGSARRRGAKSASPGRRLSTARAHAEDAEGAKGRVQSPA
  VEEPSWTPLPTPLESPAPPAGRSLVQRDIQAFLNQCGASPGEARHWLTQFQTCYHSVDKPFAVMEVDEEV
  IRCPQAVSRLAFALAFLQRMDMKPLVVLGLPTPTAPSGCLSFWEAKAQLAQSCKVLVDELRHNAATAVPF
  FGGGSVLSAAEPAPHASYGGIVAVETDLLQWCLESNSIPILCPIGETAARRSVLLDSLEVTASLAKALQP
  TKIIFLNNSGGLRNNSQKILSNVNLPADLDLVTNAEWLSIKERQQIRLIVDVLSRLPHYSSAVITAASTL
  LTELFSNKGCGTLFKNAERMLRVRNLDSLDQGRLVNLVNASFGKKLREDYLESLRPRLHSIYVSEGYNAA
  AILTVEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQ
  WIFFWFGLADIRDSYELVNHAKGLPDSFCKPASDPGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145829
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145829.1, NM_145829.2, NP_665828.1
• RefSeq Size: 2122
• RefSeq ORF: 1584
• Locus ID: 217214
• MW: 57.5 kDa
• Gene Summary: Plays a role in the regulation of ureagenesis by producing the essential cofactor N-acetylglutamate (NAG), thus modulating carbamoylphosphate synthase I (CPSI) activity. [UniProtKB/Swiss-Prot Function]