Phb (NM_008831) Mouse Tagged ORF Clone

CAT#: MR220824

Phb (Myc-DDK-tagged) - Mouse prohibitin (Phb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phb
• Synonyms: Bap32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220824 representing NM_008831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAAAGTGTTTGAGTCCATTGGAAAGTTCGGCCTGGCGTTGGCAGTTGCAGGAGGCGTGGTGA
  ACTCTGCTTTGTATAATGTGGATGCTGGACACAGAGCTGTCATCTTTGACCGATTCCGTGGCGTACAGGA
  CATTGTGGTCGGGGAAGGGACTCATTTCCTCATCCCTTGGGTACAGAAACCAATTATCTTTGACTGCCGC
  TCTCGACCACGGAACGTACCAGTCATCACTGGTAGCAAAGACTTGCAGAACGTCAATATCACACTGCGAA
  TCCTCTTCCGGCCGGTGGCCAGCCAGCTTCCTCGTATCTACACCAGCATTGGCGAGGACTATGATGAGCG
  GGTCCTGCCTTCTATCACCACAGAGATCCTCAAGTCGGTGGTGGCTCGATTCGATGCTGGAGAATTGATT
  ACCCAGCGAGAGCTGGTCTCCAGGCAGGTGAGCGATGACCTCACAGAGCGAGCGGCAACATTTGGGCTTA
  TCCTGGATGACGTGTCCCTGACACATCTGACCTTCGGGAAGGAGTTCACAGAGGCAGTAGAAGCCAAACA
  GGTGGCTCAGCAGGAAGCGGAGAGAGCCAGATTTGTGGTGGAAAAGGCTGAGCAGCAGAAGAAGGCAGCC
  ATCATCTCTGCTGAGGGTGACTCCAAGGCCGCTGAGCTGATCGCCAACTCGCTGGCCACGGCAGGCGACG
  GCCTGATCGAGCTGCGGAAGCTGGAGGCCGCCGAGGACATTGCGTACCAGCTCTCCCGCTCTCGGAACAT
  CACCTACCTGCCAGCGGGCCAGTCCGTGCTTCTCCAGCTTCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220824 representing NM_008831
  Red=Cloning site Green=Tags(s)
  
  MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCR
  SRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIYTSIGEDYDERVLPSITTEILKSVVARFDAGELI
  TQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAA
  IISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008831
• ORF Size: 816 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008831.1, NM_008831.2, NM_008831.3, NM_008831.4, NP_032857.1
• RefSeq Size: 1815
• RefSeq ORF: 819
• Locus ID: 18673
• MW: 30.3 kDa