Kcnk15 (NM_001030292) Mouse Tagged ORF Clone

CAT#: MR221240

Kcnk15 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 15 (Kcnk15)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnk15
• Synonyms: KCNK11; KCNK14; KT3.3; TASK5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221240 representing NM_001030292
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGCCGTCGGGGCGCACGGAAACCGGCACTCGGAGCGGGAGAGCCGCGATGAGGAAGCAGA
  GCGCGCGCACGGCGGCGCTCATTCTGTGCATCCTGTCCTACCTGCTGGTGGGTGCCGCTGTTTTCGATGC
  GCTGGAATCCGAGGCGGAGCGCAGCCGGCAGCGGCTGCTGGCTCGGAAGCGGGGCGAGTTCCGCAGAAAG
  TACCGCTTCTCCGCCGACGACTACCGCGAGCTGGAGCGCCTGGCGCTGCAGGCCGAGCCGCACCGCGCCG
  GCCGCCAGTGGCGCTTCGCAGGCTCCTTCTACTTCGCCATCACGGTCATCACCACCATCGGGTATGGTCA
  TGCCGCGCCGGGCACGGACTCGGGCAAAGTGTTCTGCATGTTCTATGCCCTCCTGGGCATACCCCTGACG
  CTGGTCACCTTCCAGAGCCTGGGCGAGCGTCTAAACACACTGGTGCGGTGTCTGCTGCTGACAGCCAAGC
  GCTGCCTGGGCCTGCGGCGGCCGCACGTCTCTGCTGAGAACATGGTGGTGGCCGGGCTGCTGCTCTGCGC
  AGCCACCCTGGCCCTAGGTGCCATTGCCTTCGCGCACTTCGAGGGCTGGACCTTCTTCCACGCCTACTAC
  TACTGCTTCATCACTCTCACCACCATAGGCTTCGGCGACTTCGTGGCGTTGCAGAGAGATGAGGCGCTGC
  AGAGGAAGCCGCCCTACGTGGCCTTCAGCTTCCTCTACATCCTGCTCGGGCTCACGGTCATCGGTGCCTT
  CCTCAACCTGGTGGTCCTGCGCTTCCTCGCCAGCTCCGACGCCCCGGAACGCGCAGTCCACCGCCGTGTC
  AGCGTGTTCCGCAGGGGGGCGCCCGAGAGCCGCGTCCGCGCCGGCGCCTCCACCTGCATCCCCTACCCCT
  TCCATCAGCTGGAGACCTGGGCCCGTGACAACCCGGCCTTCTCACCCCCCTTGAGCCCAGAAGCCACGCA
  TCATTGCCACAGCAGCCCAGACAGACCCCGAGCCCGGAGGAAGTCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221240 representing NM_001030292
  Red=Cloning site Green=Tags(s)
  
  MELEPSGRTETGTRSGRAAMRKQSARTAALILCILSYLLVGAAVFDALESEAERSRQRLLARKRGEFRRK
  YRFSADDYRELERLALQAEPHRAGRQWRFAGSFYFAITVITTIGYGHAAPGTDSGKVFCMFYALLGIPLT
  LVTFQSLGERLNTLVRCLLLTAKRCLGLRRPHVSAENMVVAGLLLCAATLALGAIAFAHFEGWTFFHAYY
  YCFITLTTIGFGDFVALQRDEALQRKPPYVAFSFLYILLGLTVIGAFLNLVVLRFLASSDAPERAVHRRV
  SVFRRGAPESRVRAGASTCIPYPFHQLETWARDNPAFSPPLSPEATHHCHSSPDRPRARRKSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030292
• ORF Size: 1029 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030292.1, NP_001025463.1
• RefSeq Size: 1032 bp
• RefSeq ORF: 1032 bp
• Locus ID: 241769
• Cytogenetics: 2 H3
• MW: 38.8 kDa