Chrnb4 (NM_148944) Mouse Tagged ORF Clone

CAT#: MR221644

Chrnb4 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrnb4
• Synonyms: Acrb-4; Acrb4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221644 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGTACGCCCCTGCTCCTCGTCTCTCTGTTCGCTCTGCTTCAGCCTGGGGACTGCCGCCTGGCCA
  ACGCAGAGGAGAAGCTGATGGATGATCTCCTGAACAAAACCCGCTACAACAACCTGATCCGCCCAGCCAC
  CAGCTCCTCCCAGCTCATCTCCATCCGCCTGGAGCTATCACTGTCCCAGCTCATCAGTGTGAATGAGCGA
  GAACAGATCATGACCACCAGCATCTGGCTGAAACAGGAATGGACTGACTACCGCCTGGCCTGGAACAGCT
  CCTGCTATGAAGGGGTGAACATTCTGAGGATTCCTGCAAAGCGAGTCTGGTTGCCTGACATCGTGTTGTA
  CAACAATGCCGATGGGACCTATGAGGTGTCTGTCTACACCAACGTGATCGTGCGTTCCAACGGCAGCATC
  CAGTGGCTGCCCCCTGCCATCTACAAGAGTGCCTGCAAGATAGAGGTGAAGCACTTTCCCTTCGACCAGC
  AGAACTGCACCCTCAAATTTCGCTCCTGGACCTATGACCACACAGAGATTGACATGGTTCTTAAATCACC
  CACAGCCATCATGGATGACTTTACCCCCAGCGGTGAATGGGACATTGTGGCCCTCCCGGGTCGAAGGACA
  GTGAACCCACAGGACCCCAGCTATGTGGACGTGACCTATGACTTCATCATCAAGCGCAAGCCACTCTTCT
  ACACCATCAATCTGATCATCCCTTGTGTGCTCATCACGTCGCTGGCTATCCTGGTCTTCTACCTGCCCTC
  CGACTGTGGGGAGAAGATGACGCTCTGCATCTCAGTGCTGCTGGCACTCACATTCTTCCTGCTGCTCATC
  TCCAAGATCGTGCCTCCCACCTCCCTTGACATTCCCCTCATTGGCAAGTACCTCTTGTTCACCATGGTGC
  TGGTCACCTTTTCCATCGTCACCACTGTGTGTGTCCTCAATGTGCACCACCGTTCACCCAGCACTCACAC
  CATGGCATCCTGGGTCAAGGAGTGCTTCCTGCACAAGCTGCCCACCTTCCTCTTCATGAAGCGCCCTGGT
  CTGGAAGTCAGCCCCGCCAGGGTCCCTCATTCCAGCCAGCTGCACTTGACCACAGCTGAAGCTACGTCCA
  CCTCTGCCTTAGGCCCCAGCAGCCCATCCAACCTCTATGGGAATTCCATGTACTTTGTGAACCCTGTCCC
  TGCCACTCCTAAGTCTGCAGTCAGCTCCCACACGGCAGGCCTCCCCAGGGATGCCCGGCTGAGGTCCTCT
  GGGAGGTTCCGGCAAGATCTACAGGAAGCATTAGAGGGCGTCAGCTTCATCGCACAGCATTTGGAGAGTG
  ATGATCGAGATCAGAGTGTCATCGAGGACTGGAAATTCGTCGCAATGGTCGTCGACCGCCTGTTCCTGTG
  GGTGTTCGTGATTGTGTGTATTCTGGGCACCATGGGGCTCTTCCTGCCACCCCTCTTCCAGATCCACGCA
  CCCTCCAAGGGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221644 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGTPLLLVSLFALLQPGDCRLANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNER
  EQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSI
  QWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRT
  VNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLI
  SKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPG
  LEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSS
  GRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHA
  PSKGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148944
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_148944.1, NM_148944.2, NM_148944.3, NM_148944.4, NP_683746.1
• RefSeq Size: 3486
• RefSeq ORF: 1488
• Locus ID: 108015
• MW: 55.8 kDa