Agap2 (NM_001033263) Mouse Tagged ORF Clone

CAT#: MR221689

Agap2 (Myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agap2
• Synonyms: AGAP-2; Centg1; cnt-g1; mKIAA0167; PIKE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221689 representing NM_001033263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGGGCGCGGGCGCGCTTCAGCGCCGGACAACGACCTACCTCATCTCGCTGACCCTGGTCAAGC
  TCGAGTCGGTACCTCCGCCGCCGCCTTCTCCATCTGCAGCCGCAGCCGGCGCCCCCGGGGCCAGAGGCTC
  CGAGCCCCGAGATCCTGGCAGCCCCCGAGGCTCGGAGGAACCCGGCAAGAAGCGGCACGAGCGTCTCTTC
  CACCGGCAGGATGCGCTGTGGATCAGTACCAGCAGCGCAGGCACCGGGGGTGCGGAGCCCCCCGCGCTGT
  CCCCGGCTCCGGCCAGTCCGGCTCGCCCGGTCTCCCCGGCTCCGGGCCGCCGACTGTCCCTATGGGCCGC
  CCCTCCGGGACCCCCGCTCTCCGGGGGGCTGAGCCCGGACTCCAAGCCGGGAGGCGCCCCCTCTTCCTCC
  CGGCGCCCTCTACTCAGCAGCCCGAGCTGGGGGGGTCCGGAACCTGAAGGCCGGACGGGTGGCGGAGTCC
  CGGGCTCGTCCTCCCCGCATCCTGGCACCGGTAGCCGAAGGCTCAAGGTGGCGCCTCCTCCACCTGCTCC
  CAAGCCTTTCAAGACCGTGACCACTAGTGGAGCCAAAGCCGGCGGGGGCAAGGGCGCAGGCAGCCGCCTG
  TCATGGCCCGAAAGCGAGGGCAAACCCAGGGTCAAGGGGTCAAAGAGCACCGCCGGGACTGGAGCTTCTG
  CCGCTGCGGCCGGCGGGGGAGGGAGCGCTGCAGTCACGACCTCTGGTGGGGTCGGGGCTGGGGCTGGAAC
  CAGAGGGAAGTTGTCCCCTCGGAAAGGCAAGAGTAAGACCTTGGACAATAGTGACTTACACCCAGGACCC
  AGTGCCGGCTCTCCTCCGCTCACCGTGCCAGCAATCCCAGTTCCAGCCACTTCTGTCACTGCCACCTCCA
  CGCAGCCCCTCGGGCCTGCACCCCCTATCACTCTGGAGCCACCAGCTCCAGGGCTGAAAAGGGGCCGGGA
  GGGCGGCCGAGCGTCCACTCGTGACCGCAAGATGCTCAAGTTTATCAGCGGTATCTTCACCAAGAGCACA
  GGAGGGCCTCCTGGTCCAGGGCCCCTTCCCGGACCCCAAGGCTTGTCTTCCAGCAGCGGGTCCAGGGAGC
  TGCTGGGAGCAGAACTACGCACCTCCCCTAAGGCTGTGGTTAATAGCCAGGAATGGACTTTGAGCCGATC
  AATTCCCGAACTGCGCCTGGGTGTGCTGGGTGATGTCAGGAGTGGGAAGTCGTCCCTCATCCATAGATTC
  CTCACGGGTTCATACCAGGTGCTGGAGAAGATTGAGAGTGAGCAGTACAAGAAGGAGATGCTAGTGGATG
  GGCAGACCCATCTGGTGCTGATCCGGGAGGAAGCAGGGGCACCCGATGCCAAGTTCTCAGGCTGGGCAGA
  TGCTGTGATCTTCGTCTTCAGCCTGGAGGATGAGAGCAGCTTCCAGGCTGTGAGCCGTCTCCATGGGCAG
  CTGAGCTCCCTCCGGGGGGAGGGGCGAGGAGGTCTGGCCCTGGCTCTGGTTGGGACCCAAGACAGGATCA
  GTGCATCATCTCCTCGAGTGGTGGGTGATGCTCGAGCCAGGGCTCTGTGCACGGACATGAAGCGCTGTAG
  CTACTACGAGACCTGCGCAACCTATGGGCTCAATGTGGACCGGGTCTTCCAGGAGGTGGCCCAGAAGGTG
  GTGACCTTGCGCAAACAACAACAACTCCTGGCTGCTTGCAAATCCCTGCCTAGCTCGCCAAGCCATTCAG
  CTGCATCTACTCCTGTAGCTGGGCAGGCTAGTAACGGGGGCCACACTAGCGACTACTCTTCTTCCCTCCC
  ATCCTCACCCAACGTTGGACACCGGGAGCTCCGAGCTGAGGCAGCTGCGGTGGCTGGGTTGAGTACCCCA
  GGGTCCTTACACAGAGCAGCCAAGCGGCGGACCAGCCTTTTTGCGAACCGTCGGGGCAGCGATGCTGAGA
  AGCGGAGCTTAGACAGCCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCT
  GCTAAAACGAAGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTGACCTTGTCCAGCAATGGC
  TTTCTACTCTACCACCCCAGCATTAATGACTACATCCACAGTACACATGGCAAAGAGATGGACTTGTTAC
  GAACAACGGTCAAAGTCCCAGGCAAGCGGCCCCCAAGGGCTATCTCTGCTTTCGGCCCCTCAGCCAGCAT
  CAACGGGTTGGTCAAGGATATGAGCACCGTCCAGATGGGTGAAGGCCCTGAAGCCTCTACTCCCATGCCA
  AGCCCCAGCCCTAGCCCCAGTTCCCTGCAGCTTCCAACAGACCAGACATCCAAGCACCTCTTGAAGCCAG
  ACCGGAATTTGGCCCGTGCCCTTAGCACCGACTGTACCCCATCTGGAGATCTGAGTCCCCTGAGTCGGGA
  ACCCCCACCTTCGCCCATGGTGAAGAAGCAGAGGAGGAAGAAACTGTCCACGCCTTCTAAGACTGAAGGC
  TCGGCTGTGCAAGCTGAAGCCAAGCGCAAAATGTGGAAACTAAAATCCTTTGGTAGTTTAAGAAATATTT
  ATAAAGCAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACTGGTCAGACGTGGCACTTTGAGGCAGC
  CAGCTTTGAGGAGCGGGATGCTTGGGTTCAGGCCATTGAGAGTCAGATCCTGGCCAGTCTGCAGTGCTGT
  GAGAGCAGCAAGGTCAAGCTGCGCACTGACAGCCAAAGCGAAGCTGTGGCCATCCAGGCGATCCGCAACG
  CAAAGGGAAACTCGACCTGTGTGGACTGTGGAGCTCCCAACCCCACGTGGGCCAGTTTGAATCTGGGCGC
  ACTCATCTGCATCGAGTGTTCTGGCATCCACCGGAACCTGGGCACACACCTGTCCCGAGTGCGTTCACTG
  GATTTGGATGACTGGCCGCGGGAGCTGACCCTGGTGCTGACGGCCATTGGCAACGACACAGCCAACCGCG
  TGTGGGAGAGCGACACTCGAGGCCGTGCCAAGCCTACACGGGACTCTTCGCGGGAGGAGCGTGAGTCGTG
  GATTCGCGCCAAGTATGAGCAGCTGCTGTTCCTGGCGCCTCTGGGCACCACGGAGGAGCCGCTGGGCCGC
  CAACTGTGGGCCGCTGTGGAGGCCCAGGACGTGGCCGCCGTTCTGCTGCTTCTGGCCCACGCGCGACATG
  GGCCGCTCGACACCAGCGTGGAGGACCCGCAGCTCCGCTCGCCGCTGCACTTGGCGGCCGAGCTCGCCCA
  CGTTGTCATTACACAGCTGCTGTTGTGGTATGGCGCAGACGTGGCGGCCCGCGATGCGCAAGGCCGCACG
  GCACTGTTCTATGCCCGCCAGGCTGGCAGCCAGCTGTGTGCAGACATCCTTCTCCAGCACGGCTGCCCGG
  GAGAGGGCGGCAGCACGGCCACCACGCCCAGCGCGGCCACCACCCCCAGCATTACCGCCACGCCCAGCCC
  GAGGCGCCGGAGCAGTGCTGCCAGCTTGGGCCGCGTGGACACCACGATCGCGCTGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221689 representing NM_001033263
  Red=Cloning site Green=Tags(s)
  
  MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEEPGKKRHERLF
  HRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSS
  RRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRL
  SWPESEGKPRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGP
  SAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKST
  GGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRF
  LTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQ
  LSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKV
  VTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTP
  GSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNG
  FLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMP
  SPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEG
  SAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCC
  ESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSL
  DLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGR
  QLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRT
  ALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033263
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033263.1, NM_001033263.2, NM_001033263.3, NM_001033263.4, NM_001033263.5, NP_001028435.1
• RefSeq Size: 5645 bp
• RefSeq ORF: 3561 bp
• Locus ID: 216439
• Cytogenetics: 10 D3
• MW: 124.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the centaurin GTPase family. This gene product regulates the activity of multiple kinases, including PI3K. Reduced expression of this gene results in multiple defects, including neural deficiencies, while increased expression of this gene has been observed in some tumors. Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]