Arhgef1 (NM_001130153) Mouse Tagged ORF Clone

CAT#: MR221832

Arhgef1 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef1
• Synonyms: Lbcl2; Lsc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221832 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAGTCGCCGGAGGGGCGGCCCCAGGGCCTCCCCGGTCTGGCCTGGTGTCCATCATCATCGGGG
  CGGAGGATGAGGATTTTGAGAACGAGCTGGAGGCGAACTCAGAAGATCAAAACAGCCAGTTCCAGAGCCT
  AGAGCAAGTGAAGCGCCGCCCTGCCCACCTCATGGCCCTCCTGCAGCATGTGGCCCTGCAGTTCGAGCCA
  GGACCACTGCTCTGCTGCCTGCATGCAGACATGCTGAGCTCTCTGGGCCCCAAAGAAGCCAAGAAGGCCT
  TCCTTGACTTCTATCACAGTTTCCTGGAGAAGACTGCGGTTCTACGGGTGCCGGTCCCTCCCAGTGTCGC
  TTTTGAACTTGATCGTACTCGACCTGATCTGATCTCTGAGGATGTCCAGAGGCGGTTCATACAAGAGGTG
  GTGCAGAGCCAGCAGGCAGCCGTGAGCCGTCAGCTAGAGGACTTCCGCTCTAAGCGGCTCATGGGCATGA
  CGCCCTGGGAGCAGGAACTGAGCCTGCTGGAGCCCTGGATTGGGAAAGACCGAGGCAACTATGAGGCCCG
  GGAGCGGCATGTTGCGGAGCGGCTGCTGTCCCACCTGGAGGAGACCCAGCATACCATCTCTACAGATGAA
  GAGAAAAGTGCTGCTGTGGTCACTGCCATCAGCCTGTATATGCGCCACCTTGGAGTCCGGACCAAGAGTG
  GGGACAAGAAGTCGGGAAGGAACTTCTTCCGGAAAAAGGTGATGGGGAATCGGAGGTCAGACGAACCCCC
  AAAGACAAAGAAAGGGCTGAGCAGTATCCTAGATCCTGCACGTTGGAACCGGGGAGAGCCATCCGCTCCA
  GATTGTCGACATCTAAAGGTCGAGGCTGATGCAGAGAAGCCAGGCCCTGCAGACCGGAAGGGAGGCCTGG
  GTATGTCTTCTCGGGACAGGACTGTTGGGACTCCTGGACAGGACAACCCAGGAGTCTCCCTGCACCCTCT
  GTCTACAGACAGCGTCGACTCCCGGGAACCAGGCGTGGATACCCCGCAGGAGCCAGGGGATACACCCCCA
  CAGGGCCCTACCAGCCTGGAGCCCCTGGCGCCCCCAGAGAGCACAGAGGACAATGGCGAGACTGAGAGCC
  CTGAGCCCGGAGATGATGGGGAGCCAGGACGGTCAGGCCTGGAACTGGAACCAGAAGAACCTCCTGGGTG
  GAGGGAACTCGTGCCCCCAGACACCCTGCTCAGTCTGCCCAAGAGCCAAGTGAAGCGGCAAGAGGTCATC
  AGCGAGCTGCTCGTGACTGAGGCAGCTCACGTGCGCATGCTACGGGTACTGCATGACCTCTTCTACCAGC
  CCATGGCGGATGGAGGCTTCTTCCCTCTGGACGAGCTGCAGAACATCTTCCCGAGCCTGGATGAGCTCAT
  CGAGGTGCACTCCCTGTTCCTCGATCGCTTGATGAAGCGGAGACAAGAGAGTGGCTACCTCATTGAGGAG
  ATCGGCGATGTGCTACTGGCCCGGTTCGATGGTGCTGAGGGCTCATGGTTCCAGAAGATCTCCTCCCGCT
  TCTGCAGCCGCCAGTCGTTCGCTCTAGAGCAGCTCAAAGCCAAGCAGCGCAAGGAGCCTCGGTTCTGTGC
  CTTTGTGCAGGAAGCTGAGAGCCGCCCGAGATGCCGGCGCCTACAGTTAAAGGACATGATCCCCACTGAG
  ATGCAGCGACTGACCAAGTACCCACTGCTGCTACAGAGCATCGGGCAGAACACAGAGGAGTCTACAGAAC
  GAGGGAAAGTGGAGCTTGCAGCTGAGTGCTGCCGGGAGATTCTGCACCATGTCAATCAAGCCGTCCGTGA
  CATGGAGGACCTGCTGCGGCTCAAGGATTACCAGCGGCGCCTGGACTTGACTCACCTACGGCAGAGCAGT
  GACCCTATGCTGAGCGAGTTCAAGAACCTGGACATCACTAAGAAGAAGTTGGTCCATGAAGGCCCCCTCA
  CGTGGCGAGTGACCAAAGACAAAGCTATAGAAGTGCACGTGCTCTTGCTGGACGACCTGCTGCTGCTGCT
  CCAGCGCCAGGACGAGAGGCTGCTGCTCAAGTCCCACAGCCGGACGCTGACACCTACCCCCGATGGCAAG
  ACCATGCTGCGGCCGGTGCTCCGGCTCACCTCTGCCATGACCCGAGAGGTGGCCACTGATCACAAAGCTT
  TCTACGTCATTTTTACCTGGGACCAGGAGGCCCAGATATATGAGCTGGTGGCACAGACATCTTCGGAACG
  CAAAAACTGGTGTAACCTCATCACTGAGACTGCTGGATCCCTGAAGGTCCCTGCCCCTGCCTCCCGCCTC
  AAACCCCGGCCCAGCCCAAGCAGCATCCGAGAACCCCTGCTCAGCAGCTCTGAGAATGGCACTGGAGGCG
  CAGAGATGGCTCCAGCTGATGCCAGGACAGAGCGGCTCCTCAATGACCTCCTGCCCTTCTGCAGACCAGG
  CCCAGAGGGCCAGCTTGCTGCCACAGCCCTTCAGAAAGTACTGTCCCTGAAGCAGATCCTGCTAAGCACT
  GAGGAAGACAGTGGAGCGGGGCCTCCCCGCGATGGGGATGGGGTGCCTGGTGGTAGGGCCCCCGGCCCAG
  TGCACACCCAGGAGATTGAGGAAAACTTGCTTAGCTTAGAGGTGGCCATCAGACAACTGGAGGAGTTGGA
  AGAGGAATTTTGTCGCCTAAGACCCCTCCTGTCCCAGCTTGGGGGGACTCTGTCCCCCAACCTGGCTGCA
  CCTGAACGCTCTGCTCAGACAGGCCTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221832 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANSEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEP
  GPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEV
  VQSQQAAVSRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEETQHTISTDE
  EKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAP
  DCRHLKVEADAEKPGPADRKGGLGMSSRDRTVGTPGQDNPGVSLHPLSTDSVDSREPGVDTPQEPGDTPP
  QGPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLELEPEEPPGWRELVPPDTLLSLPKSQVKRQEVI
  SELLVTEAAHVRMLRVLHDLFYQPMADGGFFPLDELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEE
  IGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTE
  MQRLTKYPLLLQSIGQNTEESTERGKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSS
  DPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAIEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGK
  TMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKNWCNLITETAGSLKVPAPASRL
  KPRPSPSSIREPLLSSSENGTGGAEMAPADARTERLLNDLLPFCRPGPEGQLAATALQKVLSLKQILLST
  EEDSGAGPPRDGDGVPGGRAPGPVHTQEIEENLLSLEVAIRQLEELEEEFCRLRPLLSQLGGTLSPNLAA
  PERSAQTGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130153
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130153.1, NP_001123625.1
• RefSeq Size: 3273
• RefSeq ORF: 2763
• Locus ID: 16801
• MW: 102.8 kDa