Sybu (NM_001032727) Mouse Tagged ORF Clone

CAT#: MR222067

Sybu (Myc-DDK-tagged) - Mouse syntabulin (syntaxin-interacting) (Sybu), transcript variant b

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sybu
• Synonyms: Golsyn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222067 representing NM_001032727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACGGTCTGCAAAGGAAACGATGACACAGGTTGTCCCAGCAGCCAGTCGGTATCGCCTGTGAAGA
  CTCCTTCAGACACAGGGCACAGTCCCATTGGCTTTTGCCCTGGAAGCGATGAAGACTTTACCAGGAAGAA
  ATGCAGGATTGGAATGGTTGGTGAGGGAAGTATTCAGTCAGCTCGTCATAAAAAAGAACCCAAGGGAGGC
  ATCATAAAGCCAGGCAGTGAAGCAGATTTTAGCTCCTCGAGCAGCACAGGCAGCATCTCGGCTCCTGAGG
  TCCACATGTCCACGACAGGAAACAAGAGAGCCTCTTTCTCACGCAACCGAGGCCCTCATGGGCGGAGCAA
  TGGAGCATCATCCCACAAGTCTGGCAGCAGCCCACCATCCCCAAGGGAAAAAGACCTTGTGTCTATGCTG
  TGCAGGAATCCACTGAGCCCCAGTAACATCCACCCTAGTTACGCCCCTTCTTCTCCAAGTAGCAGCAACT
  CCGGCTCCTACAAAGGAAGTGATTGTAGTCCAGTCATGAGAAGGTCTGGAAGATACATGTCTTGTGGCGA
  AAATCACGGTGTCAAACCCCCAAATCCAGAACAGTATTTGACTCCTCTGCAGCAGAAGGAGGTCACAGTG
  AGGCACCTGAGGACCAAGCTGAAGGAGTCTGAGCGCCGACTCCATGAGAGGGAATCTGAAATCATGGAGC
  TCAAGTCTCAGCTGGCTCGAATGAGGGAGGACTGGATTGAGGAAGAGTGCCACAGAGTGGAGGCTCAGTT
  GGCACTCAAAGAAGCCAGAAAAGAGATTAAACAGCTCAAACAGGTCATCGAGACTATGAGGAGCAGCTTG
  GCTGATAAAGATAAAGGCATTCAGAAGTACTTTGTGGACATAAACATCCAAAACAAGAAACTGGAGTCTC
  TGCTTCAAAGCATGGAGATGGCACACAATAGTTCCCTGAGGGATGAACTGTGTCTTGACTTTTCCTTTGA
  TTCCCCAGAGAAAAGTTTACCCCTGAGCAGCACATTTGACAAGTTGCCAGATGGGTTATCTCTGGAAGAA
  CAGATAACAGAGGAAGGTGCTGACAGTGAGCTTCTGGTGGGAGACAGCATGGCTGAAGGCACAGATCTGT
  TAGATGAGATGGTGACTGCCACCACCACAGAATCCAGTGGCCTGGAGTTTGTTCATTCCACTCCAGGGCC
  ACAAGCCCTCAAGGCTCTCCCCTTGGTGAGCCACGAAGAGGGCATTGCGGTGATGGAGCAAGCCGTGCAG
  ACCGACGTGGTGCCGTTCAGCCCTGCCATCTCAGAGCTCATTCAGAGTGTGCTAAAGCTGCAGGACTACT
  GTCCCACAAGCTCAGCATCTCCAGATGAATCTGGAGCTGACTCGATGGAAAGCTTCTCAGAATCTATCTC
  TGCCTTAATGCTTGATTTAACTCCAAGAAGTCCTAACTCGGCCATCCTTCTGTCTCCTGTGGAGATCCCA
  TTCAGCAAGGGGGCTATGGAAGCCCATGCAAATCGCCTCATGAGAGAGCTAGATTTTGCAGCCTACACAG
  AAGAAAGATTGGACAGTGTCCTCTCACTGTCCCAGGGCAGTGTTGTGAGGCAGTACTGGAGCAGCAATTT
  CTTGGTGGATCTACTGGCTGTGGCTGCCCCTGTGGTACCCACTGTTCTGTGGGCATTCAGTACTCAGAGA
  GGGGGTACAGATCCTGTCTACAACATCGGAGCTTTGCTCCGAGGTTGCTGTGTGGTGGCCCTACACTCAC
  TACGCCGCACCGCTTTCCACATGAAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222067 representing NM_001032727
  Red=Cloning site Green=Tags(s)
  
  MDTVCKGNDDTGCPSSQSVSPVKTPSDTGHSPIGFCPGSDEDFTRKKCRIGMVGEGSIQSARHKKEPKGG
  IIKPGSEADFSSSSSTGSISAPEVHMSTTGNKRASFSRNRGPHGRSNGASSHKSGSSPPSPREKDLVSML
  CRNPLSPSNIHPSYAPSSPSSSNSGSYKGSDCSPVMRRSGRYMSCGENHGVKPPNPEQYLTPLQQKEVTV
  RHLRTKLKESERRLHERESEIMELKSQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSL
  ADKDKGIQKYFVDINIQNKKLESLLQSMEMAHNSSLRDELCLDFSFDSPEKSLPLSSTFDKLPDGLSLEE
  QITEEGADSELLVGDSMAEGTDLLDEMVTATTTESSGLEFVHSTPGPQALKALPLVSHEEGIAVMEQAVQ
  TDVVPFSPAISELIQSVLKLQDYCPTSSASPDESGADSMESFSESISALMLDLTPRSPNSAILLSPVEIP
  FSKGAMEAHANRLMRELDFAAYTEERLDSVLSLSQGSVVRQYWSSNFLVDLLAVAAPVVPTVLWAFSTQR
  GGTDPVYNIGALLRGCCVVALHSLRRTAFHMKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032727
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032727.1, NP_001027899.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 1782 bp
• Locus ID: 319613
• Cytogenetics: 15 B3.2
• MW: 65.1 kDa