Rims1 (NM_001012623) Mouse Tagged ORF Clone

CAT#: MR222084

Rims1 (Myc-DDK-tagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rims1
• Synonyms: C030033M19Rik; mKIAA0340; Rab3ip1; Rim; RIM1; RIM1a; RIM1alpha; Serg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222084 representing NM_001012623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG
  ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA
  GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA
  AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC
  CAAGCAGCGAAGAGGGAGGCCCAGACAGAAACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAACA
  AGTGAGAAAAATCGGAGAGGAAGCCAGGCGTTACCAGGGCGAGCACAAAGATGATGCCCCGACGTGTGGA
  ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG
  CACGCTGTGGAGGCCGAGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT
  ATGTCGAAAGCAACAAGAGATATTAACGAAATCTGGAGCGTGGTTCTTCGGAAGTGGTCCTCAGCAGCCC
  AGTCAAGATGGGACTCTGAGTGACACGGCTACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCTC
  GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTTC
  CCATGGTGCACCACTGGACAGGAACAAAGGGGCGGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG
  CAGGCATCAAGGTCAAGAAGTGAGCCACCAAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG
  GCAAGGGAGGCCAGAAGAGTGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGACCGC
  GGATGAACGGGAGAGGAAGGAGAGGCGGGAAACCCGCAGGCTGGAGAAAGGGCGCTCTCAGGACTACCCG
  GACCGGCTCGAGAAACGCGAGGATGGCAGGGTGGCTGAAGACGAAAAGCAGAGGAAGGAGGAGGAGGGCG
  TGTCCACGCCGGAGTACACGAGCTGCGAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGA
  CTTGGACTACTGGTTGGATCCTGCCACGTGGCATAGCAGGGAAACGTCGCCTATTAGTTCGCATCCTGTA
  ACGTGGCAGCCATCTAAAGAGGGGGACCGATTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGC
  CCAAAGAATCAGGTGCATTATTGGGTCTCAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGTCTTGG
  TGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTGGCAGATGTTGTCGGACACCTAAGAGCAGGGGATGAA
  GTTCTAGAGTGGAATGGTAAACCCCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATTTTAGAAT
  CAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAG
  TTCCCACCCTCCTCTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCT
  GTTATTTCTCCAACCAGCCCTGGAGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGA
  AGCTGTGGTATGATAAGGTGGGGCACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAG
  AGTAGATGGGCGTCCCAGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAGAGTAAA
  AGAAGAACCAAAACGGTAAAGAAACTTCTAGAGCCAAAATGGAATCAAACATTTGTCTACTCACACGTAC
  ATCGTCGAGATTTTCGAGAGCGAATGTTAGAAATTACTGTGTGGGACCAGCCAAGAGTACAGGACGAGGA
  GAGTGAATTTCTAGGAGAGATCCTCATAGAATTGGAAACAGCACTTTTAGATGATGAGCCCCATTGGTAT
  AAACTGCAGACACATGATGAATCTTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATA
  TCCACGGAGAAAGCTCCAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTA
  TGAGGTTGATGATGGTATTGGCGTAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCC
  ACCACGTTAACAGTGCCAGAGCAGCAAAGAACCACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCG
  GCGATGATCAGGGAAGGCCTCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATCCA
  TCCAACACGAAGGTCACGTTCTCCAACCCGACACCATGATGCCTCCCGAAGCCTGGCCGATCACAGATCA
  CGACATGCGGAAAGTCAATATTCGTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAG
  GACGAAGTGCAGAATGCCTACACATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAG
  TACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAA
  AGATCTAGCATCCAAAAACAGTCTAGGAAAGGCACAGCCTCTGATGCAGACAGGACGCACCGACAAGGAA
  GCCCCACCCAGTCACCTCCAGCTGACACATCCTTTGGCAGTCGCCGCGGAAGACAGCTCCCACAGGTGCC
  GGTCCGAAGCGGCAGTATAGAACAAGCGAGCTTAGTAGTGGAGGAACGAACGAGACAGATGAAAATGAAG
  GTACACCGATTTAAGCAGACAACAGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGT
  ACAACATACATAAAGATCAGTACAGAAGCTGTGATAACGCGTCCGCCAAGTCATCAGATAGTGATGTCAG
  TGATGTGTCCGCCATTTCCAGAGCCAGCAGCACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAG
  TCTGAGCGCCCCAGGGGCAGGATCAGTTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAG
  GAAGAGCCATCATCAAGAGCACCAGTGTAAGTGGAGAGATATATACACTGGAGCACAATGACGGCAGCCA
  GTCCGACACGGCCGTGGGTACCGTTGGAGCTGGTGGAAAGAAGCGGAGGTCCAGCCTGAGTGCCAAAGTG
  GTAGCCATTGTGTCTCGAAGAAGCAGAAGCACATCACAGCTCAGCCAGACAGAGTCCGGCCACAAGAAGT
  TAAAAAGCACCATTCAGAGGAGTACGGAAACGGGAATGGCGGCTGAAATGCGGAAGATGGTGAGACAGCC
  CAGCCGGGAGTCCACGGATGGCAGCATCAACAGTTATAGCTCAGAAGGAAACTTAATATTTCCTGGAGTT
  CGCGTGGGACCTGACAGTCAGTTCAGTGATTTCCTTGATGGACTGGGGCCAGCCCAGCTTGTCGGCCGCC
  AAACCCTAGCCACCCCAGCCATGGGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGT
  TGAGGTTATCAGAGCCCGGAGCCTTACACAAAAACCTGGCTCCAAATCTACACCTGCTCCCTATGTGAAA
  GTTTATCTTTTGGAAAATGGAGCCTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATC
  CCTTGTATCAGCAGTCCCTGGTTTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGG
  TGACTATGGAAGAATGGACCACAAATGCTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTG
  TCCAGCATGGTGATTGGATGGTATAAATTGTTCCCTCCATCCTCCCTGGTGGATCCCACACTCACTCCCC
  TGACCCGCCGGGCTTCCCAATCATCACTGGAAAGTTCGTCTGGGCCTCCCTGCATCCGGTCA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222084 representing NM_001012623
  Red=Cloning site Green=Tags(s)
  
  MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTP
  RNAESQPHQPPLNIFRCVCVPRKPSSEEGGPDRNWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCG
  ICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQP
  SQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTASHGAPLDRNKGAEPSQQALGPEQK
  QASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGTADERERKERRETRRLEKGRSQDYP
  DRLEKREDGRVAEDEKQRKEEEGVSTPEYTSCEDVELESESVSEKGDLDYWLDPATWHSRETSPISSHPV
  TWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDE
  VLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSIS
  VISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSK
  RRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWY
  KLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKA
  TTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSLADHRS
  RHAESQYSSEPDSELLMLPRAKRGRSAECLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSE
  RSSIQKQSRKGTASDADRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMK
  VHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQ
  SERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLEHNDGSQSDTAVGTVGAGGKKRRSSLSAKV
  VAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGV
  RVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVK
  VYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDL
  SSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSSGPPCIRS
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012623
• ORF Size: 4122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012623.1, NP_001012641.1
• RefSeq Size: 4125 bp
• RefSeq ORF: 4125 bp
• Locus ID: 116837
• Cytogenetics: 1 A5
• MW: 153.8 kDa