Dgkh (NM_001081336) Mouse Tagged ORF Clone

CAT#: MR222176

Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgkh
• Synonyms: 5930402B05Rik; D130015C16; DGK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222176 representing NM_001081336
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCCGGCAGCCAGCACCACCCTCAGGGCGTCGCGGGAGGAGCGGTCGCTGGGGCCAGCGCGG
  TTTCCCCCACTGCTGCAGGACCGGGAGAGGATTCGTCCGACAGTGAAGCGGAGCAGGAGGGGCCCCAGAA
  GCTGATCCGTAAAGTGTCCACCTCTGGACAGATGCGGACCAAGACTAGTATTAAGGAAGGACAACTGCTG
  AAGCAAACCAGCTCTTTCCAAAGGTGGAAGAAGAGATACTTCAAGCTTCGCGGCCGCACACTTTATTATG
  CGAAAGACTCCAAGTCCTTGATATTTGATGAAGTTGACCTCTCAGATGCCAGTGTTGCGGAAGCAAGCAC
  AAAAAATGCCAACAACAGCTTTACGATCATCACTCCGTTCAGAAGACTGATGCTATGTGCTGAGAACAGG
  AAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAGAACCCTATGAGGTGGCCCAGT
  TCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGGCCCACCTTCTGTAA
  CGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGTGAAGTGTGTAAGTTCAAGGCT
  CATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGCTGGCCTCCATCGGGAAGGACA
  TCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGGCAACTTGCCTGTGAGTGCCAA
  GTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGATTGGAAATGCCTTTGGTGTAAG
  ACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCCTTGGCCAGTGTAAAGTCTCCA
  TCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCGAGCAACCTTTTCGTTCTGTGT
  CAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGAGTGAAGTTCCTTCGTCGCTTT
  AAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGCCTTATTTAGGCTTGAGATTGT
  TTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAGTGTAGGCTGGGTTTTGTCAGA
  GATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCCTTGGGTACCGGCAATGACCTT
  GCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCCCTCAGATACTAGAGAAGCTGG
  AACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGAGCTCAAATTGCCAGCAAAGTC
  GTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACAATTTATGAAGACTCCGTTGCA
  AACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATATCGTCTGCCAAGATACTCTGTG
  AAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAACACTGGAAAATACAGTAGTAGC
  CGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAGTTGCTACAAGCCTTGCACGCG
  GACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCGAAGAGGACACCGTGGAGTCCG
  CCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACATTGGGAAACCCTCCTCCCAGAA
  AGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAAGCGGTGAGGCAAGTCATTGAA
  GAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAGTCAGTCCGTCTTGTGACTATG
  ATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATCAGCCAAAACCACATCTCAGTC
  TCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCTGGTCCAGCCATGGCCACCACC
  AAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGAGAGCAGGACTTGCTGCCTCCA
  TTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCCTTTCGGGGCAACACCGTTTAT
  TGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATGAACAACTACTTTGGGATTGGA
  TTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACCCTGAAAAATGCAGAAGTCGAA
  CTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACAGAGATCGTACAAGAATCTGGA
  GCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGTCTACAAGGCATAGCGGTGTTG
  AACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAGAGGATGATATATTTGCTGCGC
  CATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGTGCAGATGGCAGTATCAAGGGT
  CATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATCACGATATTTGGCGACGAGGGA
  GTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCATCAAGATTGTGCACAAAAACA
  GAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTCTTGGGAAGACAAGCAGAAGTG
  TGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCCCCTGACCTGGCAACAGAGGAA
  GTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTAGGATTTGTGACGCAGCCACGA
  TTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTCACACGCCCTGAACAAAGCCAA
  CCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCCATCAACGTCAAGGCGCTCTAT
  AATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAATCTCCTCACGAAGAGCGGGTGT
  CCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGATTCCCTGGCTTTATTACATCCT
  CCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAACAACAAAAGCACAGTCTTTCGC
  ATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTTCGCAGCCTGGACCTGGGGATT
  CCGAAAGTGGGTCATATGAAGCGAATTCTCCAGGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222176 representing NM_001081336
  Red=Cloning site Green=Tags(s)
  
  MAGAGSQHHPQGVAGGAVAGASAVSPTAAGPGEDSSDSEAEQEGPQKLIRKVSTSGQMRTKTSIKEGQLL
  KQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENR
  KEMEVWISSLKSVQSREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKA
  HKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCK
  TMVHTACKDVYHPVCPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRF
  KQLLNPAQVFDLMNGGPYLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLHKQCQLGVLPLGTGNDL
  ARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPAKSSLLPEPVAATEEFYMTIYEDSVA
  NHLTKIVNSDEHAVVISSAKILCETVKDFVAKVEKAQDRTLENTVVAEAVASKCSVLNEKLEQLLQALHA
  DSQASRVPPGIGPAIPEEDTVESASDESLGESKDQLVNDIGKPSSQKAVKPREIMLRANSLKKAVRQVIE
  EAEKVMDEPAVQPSEPVSPSCDYDTETDEAKEDDAKESLSAKTTSQSPDAQASCGHPQTDSVAGPAMATT
  KENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSLDGYSEKCVMNNYFGIG
  LDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVL
  NIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAVFDSVQMAVSRVIKLQHHRIAQCRTVKITIFGDEG
  VPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTNLYIHPAPDLATEE
  VSQMRLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRFPESLTRDTATEIAINVKALY
  NETEALLVGRVPLHLESPHEERVSSALHSVEMELQKLTEIPWLYYILRPSEDEEPPLDCTKRNNKSTVFR
  IVPKFKKEKAQKQKTSSQPGPGDSESGSYEANSPGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081336
• ORF Size: 3468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081336.1, NP_001074805.1
• RefSeq Size: 3967
• RefSeq ORF: 3543
• Locus ID: 380921
• MW: 127.4 kDa