Ern2 (NM_012016) Mouse Tagged ORF Clone

CAT#: MR222188

Ern2 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ern2
• Synonyms: Ern1; Ire1; ire1-beta; Ire1b; mIre1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGACCAGTCCAGAGATTTCAACTGTGGTCTCCTCTCGGGTTCCTGCTGCAGCTTGTGACGTTGC
  TGGGGAAACTCGGTCCACAGGTCCAGTCTGTCAGGCCAGAGAGCCTTCTGTTTGTGTCTACCTTGGACGG
  AAGTCTCCATGCACTGAACAAGCAGACAGGGGACTTGAAGTGGACAGTGAAGGATGACCCCATCATCCAG
  GGCCCAATGTATGTCACAGAAATGGCCTTTCTCTCTGACCCAGCTGACGGCAGCCTGTATGTCTTGGGAA
  CCCAGAAACAACAGGGGCTGATGAAACTGCCATTCACCATCCCAGAGCTGGTTCATGCCTCTCCCTGTCG
  CAGCTCTGATGGTGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTAGTGGACCCTGAGTCAGGG
  GAGACTCAGATGACACTGACCACAGAAGGCCTCTCTACTCCTCAACTTTTCATTGGCCGAACACAGTACA
  CAGTCTCCATGCATGACCTACGGACCCCAGCCCTGCGGTGGAACACAACCTATCGCCGCTACTCAGCACC
  CCTCCTGAATGGCTCACCCGGGAAATACATGAGCCACCTGACATCCTGTGGGATGGGCCTGCTTCTCACT
  GTGGATCCAGGAAGTGGAATTGTGCTGTGGACACAGGACCTGGGAGTGCCTGTGACAGGGATTTACACAT
  GGCACCAGGATGGTCTGCACCAGCTGCCCCATCTCACACTGGCTCGGGACACCCTACATTTCCTTGTCCT
  CCGCTGGGGCCACATCCGTCTTCCTGCCTCCAGCTACCAAGACACAGCCACCCAGTTCTCTTCCTTAGAC
  ACCCAGCTTTTGATGACACTGTATGTGGGGAAAGAAGAAGCTGGGTTCTATGTCTCTAAAGCACTGGTCC
  ACGCTGGGGTGGCCCTCGTGCCCCGTGGACTGACCCTGGCACCCATGGACGGTCCCACAACAGATGAGGT
  GACACTTCAAGTCTCGGGGGAGCGAGAGGGCTCACCCAGCACTGCTGTCAGATACCCCTCAGGCAGCGTG
  GCCCTCCCCAGCCAGTGGCTACTCATTGGATACCACGAACCTCCCCCCGTTCTACACACAACCATGCTGC
  GGGTTCATCCCATCCCAGGGAAAGTGTCTGCTGAGACACGAGCCTCAGAGGACCTCCATGCTCCACCTGT
  CTTTTTTGAGCTCCTAAACCTGAGGAGGGAGGATCCAGAACTACATCCTGAAGAGAAAGCTTCAGACTCT
  TATCCAGGGCTGGGATCCCAAGACCTGCTAGCAGCTACCTTCACTGCCATCCTCCTGGGAGCCTGGGTCC
  TCTACCTGATGAGGCAGCAGCAGCAGAGCCCTTCAGCACCTGCTGGCCCTCCTGACCTCTCACAGGATGC
  TCAGGGACAGCTCTCAAGGGACATCTTACAGGACCAGAGGAGATTTCAAAGCCCCTCAGAGCCAGCCCAG
  CCACCTCATGACCCTGAAGGGCAGCCCACCGTGGTGGGGAAGATTTCCTTCAACCCCAAGGATGTGCTGG
  GCCGTGGGGCAGGAGGGACTTTTGTTTTCCGAGGACAGTTTGAGGGGCGGGCAGTAGCTGTGAAGAGGCT
  CCTCCGAGAGTGCTTTGGCTTGGTTCGGCGAGAGGTGCAGCTGCTGCAAGAGTCTGACCGGCACCCCAAC
  GTGCTTCGCTACTTCTGCACTGAGCATGGTCCCCAGTTCCACTACATTGCCTTGGAGCTCTGCCAGGCCT
  CCTTGCAAGAGTACGTGGAGAGCCCAGACCTAGATCGCTGGGGCCTGGAGCCCACCACAGTGCTGCAGCA
  GATGATGTCCGGCCTGGCCCATCTACATTCCTTACACATAGTACACCGTGACCTAAAGCCAGCCAACATT
  CTCATGGCTGGGCCTGACAGCCAGGGCCAAGGCAGAGTGGTCATCTCTGACTTCGGCCTCTGCAAGAAGC
  TACCTGTTGGCCGTTGTAGCTTCAGTCTCCATTCTGGTATTCCCGGCACAGAAGGCTGGATGGCACCAGA
  GCTCCTGCAGCTCCCCCCAGACAGCCCGACCAGCGCTGTGGATATCTTCTCCGCAGGCTGCGTATTTTAT
  TACGTGCTTTCCGGTGGCAGCCACCCCTTTGGAGAGAGTCTCTACCGACAGGCCAACATCCTCTCAGGGG
  ATCCCTGTCTGGCTCAGCTGCAGGAAGAGACCCATGACAAGGTTGTGGCATTGGACTTAGTGAGAGCCAT
  GCTGAGCTTGCTGCCCCAGGATCGCCCCTCGGCAGGATGGGTACTGGCTCACCCCCTCTTTTGGAGCAGA
  GCTAAGGAGCTCCAGTTCTTCCAGGATGTCAGTGACTGGTTGGAGAAGGAACCAGATCAGGGGCCTCTCG
  TGTCGGCGCTGGAGGCAGGAAGCTACAAGGTGGTCCGGGAAGACTGGCACAAGCACATCTCAGCCCCTCT
  GCAGGCAGATCTGAAAAGGTTCCGCTCATACAAAGGGACATCAGTTCGAGACCTGCTCCGGGCCATGAGG
  AACAAGAAGCACCACTACAGGGAGCTCCCAGCTGAGGTGCGCCAGACACTAGGCCAACTTCCTGCTGGCT
  TCATTCAGTACTTCACACAGCGATTCCCCAGGCTGTTGCTGCACACTCACCGTGCCATGAGGACCTGCGC
  CTCTGAGAGCCTCTTCCTGCCCTACTACCCACCAGCTCTGGAAGCCAGGAGGCCAGATGCCACAAAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARPVQRFQLWSPLGFLLQLVTLLGKLGPQVQSVRPESLLFVSTLDGSLHALNKQTGDLKWTVKDDPIIQ
  GPMYVTEMAFLSDPADGSLYVLGTQKQQGLMKLPFTIPELVHASPCRSSDGVFYTGRKQDAWFVVDPESG
  ETQMTLTTEGLSTPQLFIGRTQYTVSMHDLRTPALRWNTTYRRYSAPLLNGSPGKYMSHLTSCGMGLLLT
  VDPGSGIVLWTQDLGVPVTGIYTWHQDGLHQLPHLTLARDTLHFLVLRWGHIRLPASSYQDTATQFSSLD
  TQLLMTLYVGKEEAGFYVSKALVHAGVALVPRGLTLAPMDGPTTDEVTLQVSGEREGSPSTAVRYPSGSV
  ALPSQWLLIGYHEPPPVLHTTMLRVHPIPGKVSAETRASEDLHAPPVFFELLNLRREDPELHPEEKASDS
  YPGLGSQDLLAATFTAILLGAWVLYLMRQQQQSPSAPAGPPDLSQDAQGQLSRDILQDQRRFQSPSEPAQ
  PPHDPEGQPTVVGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPN
  VLRYFCTEHGPQFHYIALELCQASLQEYVESPDLDRWGLEPTTVLQQMMSGLAHLHSLHIVHRDLKPANI
  LMAGPDSQGQGRVVISDFGLCKKLPVGRCSFSLHSGIPGTEGWMAPELLQLPPDSPTSAVDIFSAGCVFY
  YVLSGGSHPFGESLYRQANILSGDPCLAQLQEETHDKVVALDLVRAMLSLLPQDRPSAGWVLAHPLFWSR
  AKELQFFQDVSDWLEKEPDQGPLVSALEAGSYKVVREDWHKHISAPLQADLKRFRSYKGTSVRDLLRAMR
  NKKHHYRELPAEVRQTLGQLPAGFIQYFTQRFPRLLLHTHRAMRTCASESLFLPYYPPALEARRPDATKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012016
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012016.1, NM_012016.2, NM_012016.3, NP_036146.2
• RefSeq Size: 2973
• RefSeq ORF: 2736
• Locus ID: 26918
• MW: 101.2 kDa