Piga (NM_011081) Mouse Tagged ORF Clone

CAT#: MR222212

Piga (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class A (Piga)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Piga
• Synonyms: AI194334; Pig-a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222212 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAATAGAAGAGGAGGTGGGCAAGGCCAGCCTCCTTCAGTGTCGCCTTCCCCGGGTAGCTCTGGAA
  ACCTTTCCGACGATAGGACCTGCACCCATAATATATGCATGGTGTCAGACTTTTTCTACCCAAACATGGG
  AGGCGTGGAAAGCCACATTTACCAGCTCTCTCAGTGCCTCATTGAGAGAGGGCACAAGGTCATAACTGTC
  ACCCATGCTTATGGAAATCGAAAGGGCGTCCGTTACCTCACCAATGGCCTCAAAGTTTACTATTTGCCTC
  TGAGAGTCATGTACAACCAATCTACAGCCACGACTCTCTTTCACAGTCTGCCATTGCTCAGGTACATATT
  TGTTCGGGAGAGAATTACGATAATCCATTCCCACAGTTCTTTCTCTGCCATGGCCCATGATGCTCTCTTC
  CACGCCAAGACAATGGGGCTTCAGACAGTCTTCACAGACCATTCCCTTTTCGGATTTGCTGATGTCAGCT
  CGGTGCTTACAAACAAGCTTCTAACTGTGTCTCTTTGTGACACAAACCACATCATTTGTGTCTCTTACAC
  TAGTAAGGAGAACACTGTACTCAGAGCAGCACTGAATCCTGAAATAGTGTCCGTCATTCCTAACGCTGTA
  GATCCTACTGACTTCACTCCAGACCCATTTAGGAGGCATGATAGTGTAATAACTGTTGTTGTTGTCAGCA
  GACTTGTTTACAGAAAAGGGACTGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATACCAAGA
  ATTACATTTCCTAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTACGGGAAAGATACCAA
  CTACATGACAGGGTGCAGCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAATGTCTTAGTTCAAGGAC
  ACATTTTTCTTAACACCTCCCTCACTGAAGCGTTCTGCATGGCCATCGTGGAAGCTGCCAGTTGTGGTTT
  GCAGGTCGTCAGCACAAAGGTTGGTGGGATTCCCGAAGTCCTTCCGGAAAGTCTTATTATTCTCTGCGAG
  CCTTCAGTAAAATCTTTGTGTGACGGTCTGGAAAAAGCCATTTTCCAAGTGAAGTCGGGGACATTGCCAG
  CTCCAGAAAATATCCACAATGTAGTGAAGACTTTCTACACCTGGCGGAACGTGGCAGAGAGAACTGAAAA
  GGTGTATGAGCGAGTGTCGAAGGAAACTGTTTTGCCAATGCACAAGAGATTGGACAGGCTCATCTCACAC
  TGTGGCCCTGTGACGGGCTACATGTTTGCTTTGCTGGCTGTGCTCAGCTATCTCTTCCTCATTTTCCTGC
  AGTGGATGACTCCAGACTCTTTCATTGATGTTGCAATAGATGCTACTGGGCCAAGGAGAGCCTGGACTCA
  TCAATGGCCTCGTGATAAGAAAAGAGATGAGAATGATAAGATATCCCAAAGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222212 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITV
  THAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALF
  HAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAV
  DPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQ
  LHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCE
  PSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISH
  CGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011081
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011081.1, NM_011081.2, NP_035211.2
• RefSeq Size: 3593
• RefSeq ORF: 1458
• Locus ID: 18700
• MW: 54.5 kDa
• Gene Summary: Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis. [UniProtKB/Swiss-Prot Function]