Hsd17b1 (NM_010475) Mouse Tagged ORF Clone

CAT#: MR222239

Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd17b1
• Synonyms: 17beta-HSD; 17HSDB1; E2DH; Hsd17ba
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222239 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCACGGTAGTGCTCATTACCGGTTGCTCCTCTGGAATCGGCATGCACTTGGCTGTTCGCCTAG
  CTTCTGACCGTTCCCAGAGCTTCAAAGTTTATGCCACATTGCGGGACCTGAAGGCACAGGGACCATTGTT
  GGAGGCCGCCAGGACTCAAGGATGCCCTCCTGGCTCCTTGGAGATACTGGAATTGGATGTCAGAGACTCC
  AAATCTGTGGCTGCTGCCCAGGCATGCGTGACTGAGGGTCGTGTGGATGTACTGGTCTGTAATGCTGGCC
  GCGGCTTGTTTGGGCCGCTAGAAGCACATGAATTGAACGCTGTGGGTGCTGTGTTGGATGTGAATGTGCT
  TGGGACCATTCGGATGCTGCAGGCCTTTCTGCCAGACATGAAGAGGCGCCACTCTGGGCGTGTGCTGGTG
  ACCGCGAGTGTGGGAGGCTTGATGGGACTGCCATTCCACGAAGTGTACTGTGCCAGCAAGTTTGCGCTCG
  AAGGTTTGTGCGAGAGTCTGGCGATCCTGCTGCCGCTCTTTGGAGTCCATGTGAGCCTCATCGAGTGTGG
  GGCAGTGCACACAGCCTTCTATGAAAAGCTGGTGGGCGGCCCCGGCGGGGCTCTGGAACGAGCAGATGCC
  CAGACCCGCCACCTCTTTGCGCACTACCTGCGTGGTTATGAGCAAGCCCTGAGCGAGGCGCAGGACCCAG
  AGGAGGTGACGGAGCTCTTCTTGACTGCAATGCGAGCCCCGCAGCCCGCGCTGCGCTACTTCTCCACGAA
  CCGCTTCCTACCCCTGGCCCGAATGCGCACAGAGGATCCCAGTGGCAGCAGCTATGTCGCGGCCATGCAC
  CAAGAAGCCTTCTCTAATCTGCAGACCCAGGAGAATGCCAAGGCTGGGGCCCAAGTCCCGGGGGTCTCGG
  ACACGGCCTCCAGCGCCCTCATTTGCCTCCCTGAATGTGCAATCCCTAGGGTGGCATCTGAATTGGGGTG
  GTCTGCATCGGATAAACCGGGTCAGGACAATTCATGTTACCAGCAAAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222239 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPTVVLITGCSSGIGMHLAVRLASDRSQSFKVYATLRDLKAQGPLLEAARTQGCPPGSLEILELDVRDS
  KSVAAAQACVTEGRVDVLVCNAGRGLFGPLEAHELNAVGAVLDVNVLGTIRMLQAFLPDMKRRHSGRVLV
  TASVGGLMGLPFHEVYCASKFALEGLCESLAILLPLFGVHVSLIECGAVHTAFYEKLVGGPGGALERADA
  QTRHLFAHYLRGYEQALSEAQDPEEVTELFLTAMRAPQPALRYFSTNRFLPLARMRTEDPSGSSYVAAMH
  QEAFSNLQTQENAKAGAQVPGVSDTASSALICLPECAIPRVASELGWSASDKPGQDNSCYQQKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010475
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010475.1, NP_034605.1
• RefSeq Size: 1317
• RefSeq ORF: 1035
• Locus ID: 15485
• MW: 36.8 kDa
• Gene Summary: Favors the reduction of estrogens and androgens. Uses preferentially NADH. [UniProtKB/Swiss-Prot Function]