Ltk (NM_203345) Mouse Tagged ORF Clone

CAT#: MR222629

Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ltk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222629 representing NM_203345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTCCCACCGGCTGCTGCTGTGGCTGGGAGCTGCTGGAACTATTCTTTGCTCCAACTCGGAGT
  TCCAGACACCTTTTCTAACACCTTCGCTTTTGCCGGTGCTGGTACTCAATTCCCAGGAGCAGAAAGTCAC
  CCCCACACCCAGTAAATTGGAGCCAGCTTCCCTCCCAAATCCTCTAGGCACACGGGGGCCTTGGGTGTTT
  AACACCTGTGGCGCCAGCGGCAGGAGCGGACCCACACAAACACAGTGCGATGGGGCATACACAGGAAGCA
  GCGTGATGGTGACAGTGGGGGCTGCCGGGCCGCTCAAAGGCGTGCAGCTGTGGCGGGTGCCAGACACAGG
  CCAGTATCTGATCTCCGCCTACGGAGCGGCGGGCGGCAAGGGCGCCCAAAACCACCTGTCACGGGCGCAC
  GGCATCTTCCTCTCAGCAGTCTTCTTCCTCCGTCGCGGGGAGCCGGTGTACATCCTTGTGGGGCAGCAGG
  GCCAGGACGCCTGTCCCGGAGGGAGCCCTGAGAGCCAACTCGTCTGTCTGGGAGAGTCTGGGGAGCATGC
  AACCACCTATGGGACCGAAAGGATCCCAGGCTGGAGACGCTGGGCCGGCGGGGGCGGGGGTGGCGGAGGC
  GCCACCTCCATCTTCCGGCTGCGCGCGGGGGAGCCAGAGCCGCTGCTGGTGGCGGCGGGAGGCGGCGGGA
  GGTCCTACCGGAGGCGACCTGACCGCGGCCGGACTCAGGCCGTCCCCGAGAGGCTGGAGACCCGCGCGGC
  GGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGTGGAGGGAGCGGCTGGACGTCGCGAGCCCACTCT
  CCGCAGGCCGGACGCTCGCCGCGGGAAGGGGCCGAGGGCGGCGAGGGCTGCGCGGAGGCCTGGGCTGCGC
  TCCGCTGGGCTGCGGCGGGAGGCTTCGGGGGCGGCGGCGGGGCCTGCGCGGCGGGCGGCGGTGGCGGCGG
  CTACCGGGGCGGTGATACTTCTGAGTCTGACCTCCTCTGGGCTGATGGGGAAGATGGCACATCCTTTGTC
  CACCCCAGTGGTGAGCTCTACCTACAGCCTCTGGCAGTCACAGAGGGCCATGGGGAGGTGGAGATCCGAA
  AGCATCCCAACTGCAGTCACTGCCCTTTCAAAGACTGCCAGTGGCAGGCAGAGCTCTGGACGGCCGAATG
  CACGTGCCCAGAGGGCACGGAGCTAGCTGTGGATAATGTCACTTGCATGGACCTGCCAACCACCGCAAGC
  CCTCTGATCCTGATGGGAGCTGTAGTGGCAGCCTTGGCACTGAGTCTCCTAATGATGTGTGCAGTCCTGA
  TTCTAGTGAACCAGAAGTGTCAGGGCCTGTGGGGGACCAGGCTGCCAGGCCCTGAGCTTGAGCTAAGCAA
  GCTTCGATCCTCTGCCATCAGGACAGCACCCAACCCTTACTATTGTCAGGTGGGACTCAGTCCTGCCCAG
  CCCTGGCCTTTGCCCCCAGGGCTCACTGAGGTTTCACCAGCCAATGTCACTCTACTCAGAGCCCTTGGCC
  ATGGTGCCTTTGGGGAAGTGTACGAGGGACTAGTGACTGGTCTTCCTGGGGACTCCAGTCCTCTTCCAGT
  GGCTATTAAGACTCTGCCAGAGCTCTGCTCCCATCAGGATGAGCTGGATTTTCTCATGGAGGCTCTGATC
  ATCAGCAAGTTCAGCCATCAGAACATTGTACGCTGTGTGGGGCTCAGCTTTCGGTCTGCCCCGCGCCTCA
  TTCTGCTGGAGCTGATGTCTGGTGGGGACATGAAGAGCTTTTTGAGGCACAGCAGACCACACCCAGGACA
  ACTGGCACCTCTGACCATGCAGGACCTATTGCAGCTGGCCCAGGATATAGCCCAGGGCTGCCACTACCTG
  GAGGAAAATCACTTCATTCACAGAGACATTGCTGCCCGTAACTGTCTGCTTAGCTGCAGTGGAGCCAGCC
  GAGTGGCCAAGATTGGAGATTTTGGAATGGCAAGAGATATCTACCAGGCCAGTTATTATCGCAAGGGTGG
  CCGGACCTTGCTCCCAGTCAAGTGGATGCCGCCAGAAGCTCTCCTGGAGGGCCTTTTCACATCCAAGACA
  GACTCCTGGTCTTTTGGGGTCCTGCTCTGGGAGATCTTCTCACTGGGGTATATGCCCTACCCTGGACATA
  CCAACCAGGAGGTTCTAGACTTCATTGCCACAGGGAACAGGATGGACCCTCCTAGGAACTGTCCTGGGCC
  AGTGTACCGAATCATGACCCAGTGTTGGCAGCATCAGCCGGAGCTCCGCCCTGACTTTGGCAGCATCTTG
  GAACGGATTCAGTACTGCACTCAGGACCCTGATGTGCTGAACTCACCCCTGCCCGTGGAACCTGGGCCCA
  TTCTAGAGGAGGAAGAGGCCTCCAGGCTGGGAAACAGGTCACTGGAGGGTCTTAGATCCCCAAAGCCCCT
  AGAGCTGAGTTCTCAGAACTTGAAGAGCTGGGGAGGAGGCCTTCTTGGCTCTTGGCTGCCCTCTGGCCTC
  AAGACCCTCAAACCCAGGTGCCTCCAACCTCAGAACATTTGGAACCCCACCTATGGCTCCTGGACCCCAA
  GGGGCCCCCAGGGTGAAGATACAGGCATTGAACACTGCAATGGCTCCTCCTCAAGTTCCATTCCAGGCAT
  CCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222629 representing NM_203345
  Red=Cloning site Green=Tags(s)
  
  MGCSHRLLLWLGAAGTILCSNSEFQTPFLTPSLLPVLVLNSQEQKVTPTPSKLEPASLPNPLGTRGPWVF
  NTCGASGRSGPTQTQCDGAYTGSSVMVTVGAAGPLKGVQLWRVPDTGQYLISAYGAAGGKGAQNHLSRAH
  GIFLSAVFFLRRGEPVYILVGQQGQDACPGGSPESQLVCLGESGEHATTYGTERIPGWRRWAGGGGGGGG
  ATSIFRLRAGEPEPLLVAAGGGGRSYRRRPDRGRTQAVPERLETRAAAPGSGGRGGAAGGGSGWTSRAHS
  PQAGRSPREGAEGGEGCAEAWAALRWAAAGGFGGGGGACAAGGGGGGYRGGDTSESDLLWADGEDGTSFV
  HPSGELYLQPLAVTEGHGEVEIRKHPNCSHCPFKDCQWQAELWTAECTCPEGTELAVDNVTCMDLPTTAS
  PLILMGAVVAALALSLLMMCAVLILVNQKCQGLWGTRLPGPELELSKLRSSAIRTAPNPYYCQVGLSPAQ
  PWPLPPGLTEVSPANVTLLRALGHGAFGEVYEGLVTGLPGDSSPLPVAIKTLPELCSHQDELDFLMEALI
  ISKFSHQNIVRCVGLSFRSAPRLILLELMSGGDMKSFLRHSRPHPGQLAPLTMQDLLQLAQDIAQGCHYL
  EENHFIHRDIAARNCLLSCSGASRVAKIGDFGMARDIYQASYYRKGGRTLLPVKWMPPEALLEGLFTSKT
  DSWSFGVLLWEIFSLGYMPYPGHTNQEVLDFIATGNRMDPPRNCPGPVYRIMTQCWQHQPELRPDFGSIL
  ERIQYCTQDPDVLNSPLPVEPGPILEEEEASRLGNRSLEGLRSPKPLELSSQNLKSWGGGLLGSWLPSGL
  KTLKPRCLQPQNIWNPTYGSWTPRGPQGEDTGIEHCNGSSSSSIPGIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203345
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_203345.1, NM_203345.2, NP_976220.2
• RefSeq Size: 3115
• RefSeq ORF: 2667
• Locus ID: 17005
• MW: 94.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]