Psen2 (NM_011183) Mouse Tagged ORF Clone

CAT#: MR222924

Psen2 (Myc-DDK-tagged) - Mouse presenilin 2 (Psen2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psen2
• Synonyms: Ad4h; ALG-3; Alg3; PS-2; PS2; Psnl2; STM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222924 representing NM_011183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCATTCATGGCCTCTGACAGCGAGGAAGAGGTGTGTGATGAGCGGACGTCCTTGATGTCAGCCG
  AGAGCCCCACATCTCGCTCCTGCCAGGAAGGCAGGCCAGGCCCGGAGGATGGAGAGAGCACTGCCCAGTG
  GAGGACTCAGGAGAGCGAAGAAGACTGTGAAGAGGACCCGGACCGCTACGCATGCAGTGGGGCTCCTGGG
  CGACCGTCGGGCCTGGAGGAAGAGCTGACCCTCAAGTATGGGGCGAAGCATGTGATCATGCTATTCGTGC
  CTGTCACGCTGTGTATGATCGTGGTGGTGGCCACTATCAAGTCTGTGCGTTTCTACACTGAGAAGAACGG
  GCAGCTCATCTACACGCCCTTCACGGAGGACACGCCCTCGGTGGGCCAGCGGCTCCTCAACTCCGTGCTT
  AACACCCTCATCATGATCAGCGTCATCGTAGTCATGACCATCTTCCTCGTGGTACTCTACAAGTATCGAT
  GCTACAAGTTCATCCATGGCTGGCTGATCATGTCCTCCCTGATGCTCCTCTTCTTGTTCACCTACATCTA
  CCTCGGGGAAGTGCTCAAGACCTACAATGTGGCCATGGACTATCCCACACTCTTCCTGGCTGTCTGGAAC
  TTCGGGGCAGTGGGCATGGTGTGCATCCACTGGAAGGGGCCTCTGGTGCTGCAGCAGGCTTACCTTATTG
  TGATCAGCGCACTCATGGCCCTGGTGTTCATCAAGTACCTGCCGGAGTGGTCTGCCTGGGTCATCTTGGG
  TGCCATCTCTGTGTACGATCTCGTGGCCGTGCTGTGCCCCAAAGGGCCACTGAGGATGCTGGTGGAAACT
  GCCCAGGAGAGAAATGAGCCCATATTTCCTGCCCTGATATACTCATCTGCCATGGTGTGGACGGTGGGCA
  TGGCAAAGCTGGACCCCTCCTCTCAAGGAGCGCTGCAGCTCCCCTATGACCCAGAGATGGAAGAAGACTC
  CTACGACAGTTTTGGAGAACCCTCATACCCTGAAGCCTTCGAAGCCCCCCTGCCTGGCTACCCAGGGGAG
  GAGCTGGAGGAGGAGGAGGAAAGGGGCGTGAAGCTCGGCCTGGGAGACTTCATCTTCTACAGCGTCCTGG
  TGGGCAAGGCTGCAGCCACTGGCAACGGAGACTGGAACACTACGCTGGCCTGTTTTATCGCCATCCTCAT
  TGGCTTGTGTCTCACCCTCCTGCTGCTTGCTGTGTTCAAGAAGGCTCTGCCCGCCCTCCCCATCTCCATC
  ACCTTTGGACTCATCTTCTACTTCTCCACAGACAACCTGGTGCGCCCTTTCATGGACACTCTGGCCTCCC
  ACCAGCTCTACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222924 representing NM_011183
  Red=Cloning site Green=Tags(s)
  
  MLAFMASDSEEEVCDERTSLMSAESPTSRSCQEGRPGPEDGESTAQWRTQESEEDCEEDPDRYACSGAPG
  RPSGLEEELTLKYGAKHVIMLFVPVTLCMIVVVATIKSVRFYTEKNGQLIYTPFTEDTPSVGQRLLNSVL
  NTLIMISVIVVMTIFLVVLYKYRCYKFIHGWLIMSSLMLLFLFTYIYLGEVLKTYNVAMDYPTLFLAVWN
  FGAVGMVCIHWKGPLVLQQAYLIVISALMALVFIKYLPEWSAWVILGAISVYDLVAVLCPKGPLRMLVET
  AQERNEPIFPALIYSSAMVWTVGMAKLDPSSQGALQLPYDPEMEEDSYDSFGEPSYPEAFEAPLPGYPGE
  ELEEEEERGVKLGLGDFIFYSVLVGKAAATGNGDWNTTLACFIAILIGLCLTLLLLAVFKKALPALPISI
  TFGLIFYFSTDNLVRPFMDTLASHQLYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011183
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011183.1, NM_011183.2, NM_011183.3, NP_035313.2
• RefSeq Size: 1991
• RefSeq ORF: 1347
• Locus ID: 19165
• MW: 50.4 kDa
• Gene Summary: This gene encodes a member of the presenilin family. Presenilins are catalytic components of the multi-subunit gamma-secretase complex, which mediates critical cellular processes through cleavage of type I transmembrane proteins including Notch receptors and the amyloid precursor protein. The encoded protein contains eight transmembrane domains and is localized to the endoplasmic reticulum, where it may play a role in calcium homeostasis. Following assembly of the gamma-secretase complex, the encoded protein is cleaved into N- and C-terminal fragments and the activated complex is released from the endoplasmic reticulum. Inactivation of this gene results in impaired synaptic function in a mouse model for Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2011]