Tfeb (NM_011549) Mouse Tagged ORF Clone

CAT#: MR223016

Tfeb (Myc-DDK-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tfeb
• Synonyms: bHLHe35; Tcfeb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223016 representing NM_011549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCTCGCTCAGTGGTCTTGGGCAAATCCCTTCTGCCCGGACTCAGTTTCTCCTTGTGCACAAT
  GGGAGCAACCATACTTATGCCAGCCTGTGCTTAAAGACTACGAAGATGATGAATACTTCATGGGCCTGTC
  TCCCCTCGACTACAGGGAGCCCGAACCAACAGCTGCCATGGCGTCACGCATCGGGCTGCGCATGCAGCTC
  ATGCGGGAGCAGGCCCAGCAGGAGGAGCAGCGAGAGCGCATGCAGCAGCAGGCTGTCATGCATTATATGC
  AACAGCAGCAGCAGCAGCAGCAGCAGCTGGGTGGGCCCCCCACCCCAGCCATCAACACCCCTGTCCACTT
  CCAGTCGCCCCCGCCTGTGCCCGGGGAGGTGCTGAAGGTGCAGTCCTACCTGGAGAACCCCACCTCCTAC
  CACCTGCAACAGTCCCAGCATCAGAAGGTTCGGAAGTATCTGTCTGAGACCTATGGGAACAAGTTTGCTG
  CCCACGTGAGCCCAGCCCAAGGTTCCCCGAAGCCTGCCCCAGCAGCATCCCCAGGGGTGCGGGCTGGACA
  CGTACTGTCCACCTCGGCCGGCAACAGTGCTCCCAACAGTCCCATGGCCATGCTACATATCAGCTCCAAC
  CCCGAGAAAGAGTTTGATGATGTCATTGACAACATTATGCGCCTGGACAGCGTGCTGGGCTACATCAACC
  CTGAGATGCAGATGCCTAACACGCTGCCCCTGTCTAGCAGCCACCTGAACGTGTACAGCGGTGACCCCCA
  GGTCACAGCCTCCATGGTGGGTGTCACCAGCAGCTCCTGCCCTGCCGACCTGACTCAGAAGCGAGAGCTA
  ACAGATGCTGAGAGCAGAGCCCTGGCCAAGGAGCGGCAGAAGAAAGACAATCACAACCTAATTGAGAGAA
  GACGCAGGTTCAACATCAATGACCGGATCAAGGAGCTGGGAATGCTGATCCCCAAGGCCAACGACCTGGA
  CGTGCGCTGGAACAAAGGCACCATCCTCAAGGCCTCTGTGGATTACATCCGGAGGATGCAGAAGGACCTG
  CAGAAGTCCCGGGAGCTGGAGAACCACTCCCGGCGCCTGGAGATGACTAACAAGCAGCTCTGGCTCCGCA
  TCCAGGAGCTGGAGATGCAGGCACGCGTGCACGGCCTCCCCACCACCTCGCCGTCGGGTGTGAATATGGC
  CGAGCTGGCCCAGCAGGTGGTGAAGCAAGAGTTGCCCAGTGAGGATGGCCCAGGGGAGGCGCTGATGCTG
  GGGCCTGAGGTCCCTGAGCCTGAGCAAATGCCGGCTCTTCCTCCCCAGGCTCCGCTGCCCTCGGCCGCCC
  AGCCACAGTCTCCGTTCCATCACCTGGACTTCAGCCATGGCCTGAGCTTTGGGGGTGGGGGCGACGAGGG
  GCCCACAGGTTACCCCGATACCCTGGGGACAGAGCACGGCTCCCCATTCCCCAACCTGTCCAAGAAGGAT
  CTGGACTTAATGCTCCTAGATGACTCCCTGCTCCCCCTGGCCTCTGACCCCCTCTTTTCTACCATGTCTC
  CTGAGGCCTCCAAGGCCAGCAGCCGCCGGAGCAGCTTCAGCATGGAGGAGGGTGATGTTCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223016 representing NM_011549
  Red=Cloning site Green=Tags(s)
  
  MAQLAQWSWANPFCPDSVSPCAQWEQPYLCQPVLKDYEDDEYFMGLSPLDYREPEPTAAMASRIGLRMQL
  MREQAQQEEQRERMQQQAVMHYMQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSY
  HLQQSQHQKVRKYLSETYGNKFAAHVSPAQGSPKPAPAASPGVRAGHVLSTSAGNSAPNSPMAMLHISSN
  PEKEFDDVIDNIMRLDSVLGYINPEMQMPNTLPLSSSHLNVYSGDPQVTASMVGVTSSSCPADLTQKREL
  TDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDL
  QKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGVNMAELAQQVVKQELPSEDGPGEALML
  GPEVPEPEQMPALPPQAPLPSAAQPQSPFHHLDFSHGLSFGGGGDEGPTGYPDTLGTEHGSPFPNLSKKD
  LDLMLLDDSLLPLASDPLFSTMSPEASKASSRRSSFSMEEGDVL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011549
• ORF Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011549.1, NM_011549.2, NM_011549.3, NP_035679.3
• RefSeq Size: 2298
• RefSeq ORF: 1605
• Locus ID: 21425
• MW: 59.4 kDa