G6pc2 (NM_021331) Mouse Tagged ORF Clone

CAT#: MR223019

G6pc2 (Myc-DDK-tagged) - Mouse glucose-6-phosphatase, catalytic, 2 (G6pc2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G6pc2
• Synonyms: G6pc-rs; IGRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223019 representing NM_021331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCCTTCATAGGAGTGGAGTGCTTATTATTCATCATCTGCAGGAGGACTACCGGACTTACTATG
  GTTTTCTAAATTTTATGTCCAATGTTGGAGACCCCCGAAATATCTTTTCTATTTACTTCCCACTTTGGTT
  TCAGTTGAATCAGAATGTTGGAACCAAGATGATCTGGGTAGCGGTCATAGGGGACTGGTTCAATCTCATA
  TTTAAATGGATATTGTTTGGCCATCGTCCTTACTGGTGGATACAAGAAACTGAGATTTATCCAAATCATT
  CAAGCCCATGTCTTGAGCAGTTTCCTACTACGTGTGAAACAGGCCCAGGAAGTCCATCTGGCCACGCAAT
  GGGCTCATCGTGCGTCTGGTATGTCATGGTAACAGCTGCCCTAAGCTACACCATCAGCCGGATGGAGGAG
  TCCTCTGTCACTCTGCACAGACTGACCTGGTCCTTTCTGTGGAGTGTTTTCTGGTTGATTCAAATCAGCG
  TCTGCATCTCAAGAGTATTCATAGCCACACATTTCCCCCATCAGGTCATTCTTGGAGTGATTGGTGGGAT
  GCTAGTAGCCGAGGCCTTTGAACACACTCCAGGAGTCCACATGGCCAGCTTGAGTGTGTACCTGAAGACC
  AACGTCTTCCTCTTCCTGTTTGCCCTCGGCTTTTACCTGCTTCTCCGACTGTTCGGTATTGACCTGCTGT
  GGTCCGTGCCCATCGCCAAAAAGTGGTGTGCCAACCCAGACTGGATCCACATTGACAGCACGCCTTTTGC
  TGGACTCGTGAGAAACCTCGGGGTCCTCTTTGGCTTGGGTTTCGCCATCAACTCAGAAATGTTCCTTCGG
  AGCTGCCAGGGAGAAAATGGCACCAAGCCGAGCTTCCGCTTGCTCTGTGCTCTGACCTCACTGACCACAA
  TGCAACTTTATCGCTTCATCAAGATCCCGACTCACGCGGAACCTTTATTTTACCTGTTGTCTTTCTGTAA
  AAGTGCGTCCATCCCCCTGATGGTGGTGGCTCTAATTCCCTACTGTGTACATATGTTAATGAGACCCGGT
  GACAAGAAGACTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223019 representing NM_021331
  Red=Cloning site Green=Tags(s)
  
  MDFLHRSGVLIIHHLQEDYRTYYGFLNFMSNVGDPRNIFSIYFPLWFQLNQNVGTKMIWVAVIGDWFNLI
  FKWILFGHRPYWWIQETEIYPNHSSPCLEQFPTTCETGPGSPSGHAMGSSCVWYVMVTAALSYTISRMEE
  SSVTLHRLTWSFLWSVFWLIQISVCISRVFIATHFPHQVILGVIGGMLVAEAFEHTPGVHMASLSVYLKT
  NVFLFLFALGFYLLLRLFGIDLLWSVPIAKKWCANPDWIHIDSTPFAGLVRNLGVLFGLGFAINSEMFLR
  SCQGENGTKPSFRLLCALTSLTTMQLYRFIKIPTHAEPLFYLLSFCKSASIPLMVVALIPYCVHMLMRPG
  DKKTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021331
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021331.1, NM_021331.2, NM_021331.3, NM_021331.4, NP_067306.1
• RefSeq Size: 2012
• RefSeq ORF: 1068
• Locus ID: 14378
• MW: 41.1 kDa
• Gene Summary: This gene encodes an enzyme that belongs to the glucose-6-phosphatase catalytic subunit family. Members of this family catalyze the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, to release glucose into the bloodstream. The family member encoded by this gene is found specifically in pancreatic islets but has not been shown to have phosphotransferase or phosphatase activity exhibited by a similar liver enzyme. The non-obese diabetic (NOD) mouse is a model for human type 1 diabetes, an autoimmune disease in which T lymphocytes attack and destroy insulin-producing pancreatic beta cells. In NOD mice, the protein encoded by this gene is a major target of cell-mediated autoimmunity. Variations in the human and mouse genes are associated with lower fasting plasma glucose levels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]