Nfasc (NM_001160318) Mouse Tagged ORF Clone

CAT#: MR223174

Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfasc
• Synonyms: AA387016; D430023G06Rik; mKIAA0756; NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223174 representing NM_001160318
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATCGAGATTCCGATGGACCTGACCCAACCCCCAACTATCACCAAGCAGTCCGTGAAGGACCACATCGT
  GGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCCCCCAGTTTTCACTGGACT
  CGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGATCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACCCCCCACCCGGCCTCCCGT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAACGCGCGC
  TTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCCTCACCACCCGAGGAGTTG
  CAGAAAGAACGCCCAGCTTCATGTATCCCCAGGGCACATCGAGCAGTCAGATGGTTCTCCGTGGCATGGA
  CCTGCTGCTGGAATGCATTGCCTCTGGCGTCCCAACACCAGACATTGCATGGTACAAGAAAGGTGGGGAC
  CTCCCATCTAACAAGGCCAAGTTCGAGAACTTTAATAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGG
  ACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGT
  AAAGGCTGCTCCATACTGGCTGGATGAGCCCAAGAACCTGATCCTGGCTCCTGGGGAAGATGGGAGGCTG
  GTATGCCGAGCCAATGGGAACCCGAAGCCGACCGTGCAGTGGATGGTGAATGGAGAGCCTTTACAATCGG
  CACCACCCAATCCCAACCGTGAGGTAGCTGGAGACACTATCATCTTCCGGGATACTCAGATCAGCAGCAG
  GGCAGTGTACCAATGTAATACATCCAATGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTGTTA
  GATGTACCCCCTCGGATGCTGTCTGCCCGCAACCAGCTCATCAGGGTGATCCTTTATAACCGGACACGGC
  TGGACTGTCCGTTCTTTGGGTCTCCCATCCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCT
  GGATGGCGGTAACTACCACGTCTACGAAAACGGCAGTCTAGAAATCAAGATGATTCGCAAAGAGGACCAA
  GGCATCTACACCTGTGTGGCCACCAACATCCTGGGCAAAGCCGAAAATCAAGTCCGCCTGGAGGTCAAAG
  ACCCCACCAGGATCTACAGGATGCCCGAGGACCAGGTGGCCAAGAGGGGCACCACGGTGCAGCTGGAGTG
  CCGCGTGAAACATGACCCCTCCTTGAAGCTCACAGTCTCCTGGCTGAAGGACGATGAGCCACTCTACATT
  GGAAACAGGATGAAGAAGGAAGATGACTCCCTGACGATCTTCGGAGTGGCAGAGCGGGACCAGGGCAGTT
  ACACGTGTATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGACC
  AGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGG
  GATGACAACAACAGCCCTATCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGT
  GGCATGACCACTCCAGGTTCCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTA
  CCAATTCAGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGA
  ACCAGTGGGGCACCCCCTGAATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGG
  AGATCACGTGGACGCCTATGAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCG
  ACGGAGAGAGACCCGAGAGACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACG
  CCTGTCTACGTTCCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACA
  CCATCATTGGGTACTCCGGAGAAGATTATCCCAGGGCTGCGCCCACTGAAGTTAAAATCCGAGTCCTGAA
  CAGCACAGCCATCAGCCTTCAGTGGAACCGAGTCTACTCTGACACGGTCCAGGGCCAGCTCAGAGAGTAT
  CGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCA
  GCTTCCCTGGTGACCGTCCCCGGGGCGTGGTGGCCCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGAT
  GGTGGTGGTCAATGGGAGAGGTGACGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCAGAAGGAGTA
  CCCAGTGCCCCCAGGCGGTTCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAGTGGGACCACC
  CAGAGCACCCCAACGGAATCCTGATTGGATACATCCTCAGATACGTGCCCTTTAATGGAACCAAACTGGG
  AAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCTGTGCAGAGAGCAGACCCAGTGTCG
  CGTTACCGCTTCTCCCTCAGTGCCAGGACACAGGTGGGCTCTGGAGAAGCAGCCACAGAGGAGTCCCCAG
  CACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTG
  GTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGG
  AGTCGAGGTGGCAAGTACCCAGTGCGGGAAAAGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAG
  AAGATGGCTCATTTGACTACAGTGATGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGG
  CACCATCAAGCAGCAGGAGAGCGATGACAGCCTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAAT
  GAAGATGGCTCCTTTATTGGCCAGTACACTGTCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCT
  CAGAGGCCACATCACCAGTCAATGCCATCTATTCCCTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223174 representing NM_001160318
  Red=Cloning site Green=Tags(s)
  
  MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGD
  LPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRL
  VCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVL
  DVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQ
  GIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI
  GNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPG
  DDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYR
  TSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQT
  PVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREY
  RAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGV
  PSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVS
  RYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKR
  SRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFN
  EDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160318
• ORF Size: 3471 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160318.1, NP_001153790.1
• RefSeq Size: 9647
• RefSeq ORF: 3474
• Locus ID: 269116
• MW: 130.7 kDa
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]