Tnnt2 (NM_011619) Mouse Tagged ORF Clone

CAT#: MR223334

Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 9

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223334 representing NM_011619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAGCAAGAGGAGGCAGTGGAGG
  AGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGCCAACGTAGAAGA
  GGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAACCCAAGCCCAGC
  AGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACTTTGATGACATCC
  ACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTTCGAGAACAGGAA
  GAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAGCGGGCCGAGCAG
  CAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGGCCCGGCGTGAGG
  AGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAACATGATGCACTT
  TGGAGGGTACATCCAGAAGACAGAGCGGAAGAGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAG
  ATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCA
  AGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCA
  GAAATACGAAATCAACGTTCTGCGAAACCGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAG
  GCCAAAGTCACCGGGCGTTGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223334 representing NM_011619
  Red=Cloning site Green=Tags(s)
  
  MSDAEEVVEEYEEEQEEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEGPVEDTKPKPS
  RLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAERAEQ
  QRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKK
  ILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGK
  AKVTGRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011619
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011619.1, NM_011619.2, NM_011619.3, NP_035749.1
• RefSeq Size: 1160
• RefSeq ORF: 867
• Locus ID: 21956
• MW: 34.7 kDa
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]