Tbata (NM_001017407) Mouse Tagged ORF Clone
CAT#: MR223431
- TrueORF®
Tbata (Myc-DDK-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 5
"NM_001017407" in other vectors (4)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tbata |
Synonyms | 1700021K02Rik; AI428928; Spatial; Titest |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223431 representing NM_001017407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGTTTCTGGGGAATGTATATAAGGGGAGTTTAGCACCTCGTAGGGATGAGGTGACTAGTCCAAAGGCAG AGCCCCAGCCAGAGACGAAGCCGGAGAACCTTCCAAGGAGCCACGGGGATGTTGGGCTCCAGAAAGAGAC TGTGGTCCCAGGCATTGTGGATTTCGAGCTGATCCATGAGGAGCTGAAGACCACAAAGCCCCAAACATCA CAACCAACACCCAGTGCCTACCGCTTTGGACGCCTAAGCCACCATTCCTTCTTCTCGAGGCACCACCCCC AACCACAGCGAGTGACTCATATCCAAGATATCGCTGGGAAGCCTGTCTGCGTGGTCAGGGACGAGTTCTC TCTGTCGGCCTTGACTCAGCCCACATTCTTATCCCGCTGTCTGATGGGGATGCCCACCATCTCTGTCCCC ATTGGGGATCCACAGTCCAATCGGAACCCCCAGCTTTCTACTTCTGACACCTGGAGGAAGAAACTGAAGG ACCTGGCTTCCCGAGTGACTGTCTTCACTAAGGAAATCCAGCCAAAGCCCGATGAGGTTGGTGTTGCACA AAGAATGGAGCCTAGAAAAAAAAGGCCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223431 representing NM_001017407
Red=Cloning site Green=Tags(s) LFLGNVYKGSLAPRRDEVTSPKAEPQPETKPENLPRSHGDVGLQKETVVPGIVDFELIHEELKTTKPQTS QPTPSAYRFGRLSHHSFFSRHHPQPQRVTHIQDIAGKPVCVVRDEFSLSALTQPTFLSRCLMGMPTISVP IGDPQSNRNPQLSTSDTWRKKLKDLASRVTVFTKEIQPKPDEVGVAQRMEPRKKRPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001017407 |
ORF Size | 591 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001017407.1, NP_001017407.1 |
RefSeq Size | 932 bp |
RefSeq ORF | 594 bp |
Locus ID | 65971 |
Cytogenetics | 10 B4 |
MW | 22.6 kDa |
Gene Summary | This gene encodes a putative transcription factor that is highly expressed in thymic cortical stromal cells, and may be involved in T-cell development. Its expression is developmentally regulated in the testis, where it is restricted to the haploid round spermatids during spermatogenesis, and thus this gene may also have a role in the control of male germ cell development. Alternative splicing of this gene results in two sets of transcript variants: the variants containing 5 additional exons at the 3' end encode long isoforms that are highly expressed in the testis, while the variants lacking the 3' end exons encode short isoforms that are highly expressed in the thymus. Most of the transcripts encoding the short isoforms have been shown to initiate translation from non-AUG (CUG) start sites. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210249 | Tbata (untagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 5, (10ug) |
USD 420.00 |
|
MG223431 | Tbata (GFP-tagged) - Mouse thymus brain and testes associated (Tbata) transcript variant 5, (10ug) |
USD 230.00 |
|
MR223431L3 | Lenti ORF clone of Tbata (Myc-DDK-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 5 |
USD 410.00 |
|
MR223431L4 | Lenti ORF clone of Tbata (mGFP-tagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 5 |
USD 410.00 |
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