Mag (NM_010758) Mouse Tagged ORF Clone

CAT#: MR223754

Mag (Myc-DDK-tagged) - Mouse myelin-associated glycoprotein (Mag)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mag
• Synonyms: Gma; siglec-4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223754 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATATTCCTCGCCACCCTGCCGCTGTTTTGGATAATGATTTCAGCTTCTCGAGGGGGCCACTGGGGTG
  CCTGGATGCCCTCGACCATCTCAGCCTTCGAGGGCACGTGTGTCTCCATTCCCTGCCGTTTCGACTTCCC
  CGATGAGCTCAGACCGGCTGTGGTACATGGCGTCTGGTATTTCAATAGTCCCTACCCCAAGAACTACCCA
  CCGGTGGTCTTCAAGTCCCGCACACAAGTGGTCCATGAGAGTTTCCAGGGCCGCAGCCGCCTATTGGGAG
  ACCTGGGCCTACGAAACTGTACCCTGCTTCTCAGCACACTGAGCCCCGAGCTGGGAGGCAAATACTATTT
  CCGAGGCGACCTGGGTGGCTACAACCAGTACACCTTCTCGGAGCACAGCGTCCTGGACATCGTCAACACC
  CCCAACATTGTGGTTCCCCCGGAAGTGGTGGCAGGAACGGAAGTGGAGGTCAGTTGTATGGTGCCGGACA
  ACTGCCCAGAGCTGCGGCCAGAGCTGAGCTGGCTGGGCCACGAGGGGCTGGGAGAGCCCACTGTGCTGGG
  TCGGCTGCGTGAGGATGAAGGCACCTGGGTGCAGGTGTCGCTGCTACACTTCGTGCCTACTAGAGAGGCC
  AACGGCCACCGTCTGGGCTGTCAGGCTGCCTTCCCCAACACCACCTTGCAGTTCGAGGGTTACGCCAGTT
  TGGACGTCAAGTACCCCCCAGTGATTGTGGAGATGAATTCCTCTGTGGAGGCCATTGAGGGCTCCCATGT
  CAGCCTGCTCTGTGGGGCTGACAGCAACCCGCCGCCGCTGCTGACTTGGATGCGGGATGGGATGGTGTTG
  AGGGAGGCAGTTGCCGAGAGCCTGTACCTGGATCTGGAGGAGGTGACCCCAGGAGAGGACGGCGTCTATG
  CTTGCCTAGCAGAGAACGCCTATGGCCAGGACAACCGCACGGTGGAGCTGAGTGTCATGTATGCACCTTG
  GAAGCCCACAGTGAATGGGACGGTGGTGGCCGTAGAGGGGGAGACTGTCTCTATCCTGTGTTCCACACAG
  AGCAACCCGGACCCCATCCTTACCATCTTCAAGGAGAAGCAGATCCTAGCCACGGTCATCTATGAGAGTC
  AGCTGCAGCTGGAACTCCCTGCAGTGACCCCCGAGGATGATGGGGAATACTGGTGTGTGGCTGAGAACCA
  GTATGGCCAGAGAGCCACTGCCTTCAACCTGTCTGTGGAGTTTGCCCCCATAATCCTTCTGGAGTCACAC
  TGTGCAGCGGCCAGAGACACCGTGCAGTGTCTATGTGTGGTAAAATCCAACCCGGAACCCTCTGTGGCCT
  TTGAGCTGCCTTCCCGCAACGTGACTGTGAATGAGACGGAGAGGGAGTTTGTGTACTCCGAGCGCAGTGG
  CCTCCTGCTCACCAGCATCCTCACGATCCGGGGTCAGGCCCAAGCCCCACCCCGCGTCATTTGTACCTCC
  AGGAACCTCTATGGCACCCAGAGCCTCGAGCTGCCTTTCCAGGGAGCACACCGACTGATGTGGGCCAAAA
  TCGGTCCTGTGGGTGCTGTGGTCGCCTTTGCCATCCTGATTGCCATTGTGTGCTACATCACCCAGACGAG
  AAGAAAAAAGAATGTCACGGAGAGCTCCAGCTTCTCAGGGGGAGACAACCCTCATGTCCTGTACAGCCCC
  GAATTCAGAATCTCTGGGGCACCTGATAAGTATGAGTCCAGAGAGGTCTCTACCCGGGATTGTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223754 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIFLATLPLFWIMISASRGGHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYP
  PVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNT
  PNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREA
  NGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVL
  REAVAESLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQ
  SNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESH
  CAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTS
  RNLYGTQSLELPFQGAHRLMWAKIGPVGAVVAFAILIAIVCYITQTRRKKNVTESSSFSGGDNPHVLYSP
  EFRISGAPDKYESREVSTRDCH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010758
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010758.1, NM_010758.2, NM_010758.3, NP_034888.1
• RefSeq Size: 2427
• RefSeq ORF: 1749
• Locus ID: 17136
• MW: 64.3 kDa
• Gene Summary: This gene encodes a type I membrane protein and member of the immunoglobulin-like superfamily. It is expressed in myelinating glial cells, including oligodendrocytes of the central nervous system and Schwann cells of the peripheral nervous system. Mice lacking the encoded protein express abundant myelin, but suffer long-term axon degeneration, altered distribution of channels and adhesion molecules at nodes of Ranvier, and altered axon cytoskeletal structure. While not required for myelination, the encoded protein enhances axon-myelin stability, helps to structure nodes of Ranvier, and regulates the axon cytoskeleton. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]