Tnnt2 (NM_001130178) Mouse Tagged ORF Clone

CAT#: MR224788

Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224788 representing NM_001130178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGTGGAAGAGCAAGAGG
  AGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGC
  CAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAA
  CCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACT
  TTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTT
  CGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAG
  CGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGG
  CCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAA
  CATGATGCACTTTGGAGGGTACATCCAGAAGCAAGCTCAGACAGAGCGGAAGAGTGGGAAGAGACAGACA
  GAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGACCACCTGAATGAAG
  ACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCTGAGAAGTTCGACCT
  GCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAACCGGATCAATGACAACCAGAAA
  GTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224788 representing NM_001130178
  Red=Cloning site Green=Tags(s)
  
  MSDAEEVVEEYEEEQEEAVEEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEGPVEDTK
  PKPSRLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRIEKRRAE
  RAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQT
  EREKKKKILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNRINDNQK
  VSKTRGKAKVTGRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130178
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130178.1, NM_001130178.2, NP_001123650.1
• RefSeq Size: 1181
• RefSeq ORF: 888
• Locus ID: 21956
• MW: 35.4 kDa
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]