Iqsec3 (NM_001033354) Mouse Tagged ORF Clone

CAT#: MR224906

Iqsec3 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 3 (Iqsec3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Iqsec3
• Synonyms: AK122446; BRAG3; mKIAA1110; synarfGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224906 representing NM_001033354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCCTGCTGGAGAACCCGGTGCGCGCCGTGCTCTACCTCAAGGAGCTCACGGCCATCGTGCAGA
  ACCAGCAAAGCCTCATCCATACCCAGCGCCAGCGCATCGATGAGCTGGAGCGGCGTCTAGACGAGCTGAG
  CGCGGAGAACCGCAGCCTGTGGGAACACCAGCAGTTGCTGCAAGCCCAGCCTCCGCCTGGGCTGGTTCCC
  CCGCCGCCGTCTGCCCCACTGCCTGCTCCTGCAGTCACTGCTCCGGCCGCCGCTGCGGCCCAGGAACCGC
  TTCAGGACCACGGACAGCTCATACCCGCTTCACCGGAGCCGCCGCTTCAGCACCACGGTCAGCTCCTGGC
  GCAGCCCCAACCTGCACCCAGCAGCAGGGTTCAGACTCCTCAGTCGCCCCACCAGCACCCGGTGGCACCC
  GGGGCCATAGCTGACAAGGAGAAGGAGCGTCCCTCGAGTTGTTGTGCTGCCGCGGGAGCCCTGCTTCAAC
  ACGCATCCCCCGCCGCCCTCGGCAAGGGCGTCCTGAGCAGGAGACCCGAGAATGAGACTGTGCTACACCA
  ATTCTGCTGCCCTGCCGCTGACACCGAGCAGAAGCCTGCCTGCTCCGACCTGGCTTCCCAAAGTGATGGC
  TCCTGTGCCCAGGCCGGTGGGGGCATGGAGGACTCCGTGGTGGCAGCGGTGGCAGCCGGCAGACCCAGTG
  CCCATGCCCCGAAGGCTCAAGCCCCGGAGCTACAGCAGGAGGAGGAGCGGCCAGGGGCAGTGGGCTCCCC
  ACGGGCTGGCCCCCTCCGCGCGGCCTCTCCTGGCCGGCAGCAGCCTGCCCTGGCGACGGCGCTCTGCTCC
  CACACTCCTGCTGCCTCCGAATACGAACTCTCCCTTGACCTAAAGAATAAACAGATTGAAATGCTAGAAC
  ACAAATACGGGGGCCACCTGGTATCCCGACGCGCCGCGTGCACCATTCAAACCGCTTTTCGCCAGTACCA
  GCTCAGCAAGAACTTTGAGAAGATCCGTAACTCGCTCCTGGAGAGCCGCCTGCCTCGGCGGATCTCACTG
  CGCAAGGTGAGGGCGCCCACAGCGGAGAGTCTGGTGGCAGAGAAGGCGCTCCTGGAGGGCTGTGGGCTCC
  TTGGGCTCCCGCTGGGGCGCTCGCCCTCGCTGCCGCCCACCTTCGCTGGCTCGCTCACCGAGCTGGAGGA
  CTCCTTTACCGAGCAGGTGCAGTCCCTGGCCAAGTCCATCGACGACGCCCTCAGCACCTGGAGCCTCAAG
  ACCATGTGCTCGCTGCAAGAGAGTGGCGCGTACCAGCTCCACCAGGCGCTGCACCCGAGTGCGGGACAGC
  CAGGCCTGGAGACCGAGGCGGCGGCGCGGGAGCCAGAGAGCGGCCCCGGGTCTGGAGATGAGGCCGGTGG
  TCTGCCTCAGGGTCACAGCGGTACCCTCATGATGGCTTTCCGGGACGTTACGGTGCAGATCGCTAACCAG
  AACATCTCTGTTTCCTCCTCCACCGCCCTGTCTGTGGCCAACTGTCTGGGTGCACAGACCGCCCAGGCCA
  CAGCCGAGCCTGCTGCCGCCCAAGCCGAACAAGAGGACACCGCGGATCAGGAGGTCTCGGAAGTCCCAGC
  CTCGGAACAGATGGACCCACCCAGTGAGGACTCAGAGGCAGCGGAGAGCCGCGCCCAGAGCGCACAGGAA
  CCCGCGGTGGCACAGGCCGTGGTGGAGGAGGCGGTGGCTACAGAGGCAGAGGAGGAAGAGGAGGGGGCCA
  AACAGGCAGGGAAGGGGGCAGAGGCCGAGGGAGGCGACAACTCTGAGCAGCTGAGCAGCAGCAGCGCTTC
  CACCAAGTCTGCCAAATCCAGCTCAGAGGCTTCTGCCGCTGCCTCCAAGGAGGCCCTGCAGGCTGTGATC
  CTGAGCTTGCCCCGCTATCACTGCGAGAACCCAGCCAGCTGCAGGTCCCCCACGCTCTCCACAGACACCC
  TGCGCAAGCGGCTGTACCGCATCGGCCTCAACCTCTTCAACATAAATCCGGACAAGGGCATCCAGTTCCT
  GATCTCACGTGGCTTCATCCCTGACACCCCCATCGGTGTGGCCCACTTCCTCCTCCAGCGTAAAGGCCTC
  AGCCGCCAGATGATAGGAGAGTTCCTGGGCAACAGCAAGAAGCAGTTCAACCGGGACGTGCTGGACTGTG
  TGGTAGATGAGATGGACTTTTCCAACATGGAGCTGGATGAAGCCCTGAGGAAGTTTCAAGCCCACATCCG
  TGTGCAGGGGGAAGCACAGAAGGTGGAGCGACTCATAGAAGCTTTCAGCCAGCGCTACTGCATGTGCAAT
  CCTGAGGTGGTGCAACAGTTCCACAACCCAGACACCATCTTCATCCTGGCCTTTGCCATCATCCTTCTCA
  ACACCGACATGTACAGCCCCAACATCAAGCCTGACCGGAAGATGATGCTAGAAGACTTTATCCGGAACCT
  CCGAGGTGTGGATGATGGTGCAGACATCCCCAGAGAACTGGTGGTAGGCATCTATGAGAGGATCCAGCAA
  AAGGAGCTCAAATCCAATGAGGACCATGTCACATATGTCACCAAGGTGGAGAAGTCCATCGTGGGCATGA
  AGACGGTGCTGTCAATGCCACACCGTCGCCTGGTCTGCTGTAGCCGGCTCTTCGAGGTGACCGATGTGAA
  CAAGCTACAGAAACAAGCAGCACACCAGAGAGAAGTGTTCCTCTTCAATGATCTGCTTGTGATTCTCAAG
  CTATGCCCAAAGAAGAAGAGCTCCTTCACGTACACCTTCTGCAAGGCAGTCGGCCTGTTAGGCATGCGCT
  TTCACCTCTTCGAGAACGAGTATTACTCCCATGGCATCACCCTGGCAACCCCACTCTCGGGCTCCGAGAA
  GAAGCAGGTGCTGCACTTCTGTGCCCTGGGCTCCGACGAGATGCAGAAGTTCGTGGAAGACCTGAAGGAG
  TCCATCGCTGAGGTGACAGAGCTGGAGCAGATCCGGATAGAATGGGAACTGGAGAAGCAGCAGGGCACAA
  AGACTCTCTCCGTAAGGTCTGCTGGAGCCCAAGGGGACCCACAGTCGAAGCAAGGATCACCCACAGCCAA
  GAGGGAGGCGATGGCAGGAGAGAAAGCGGCGGAGAGCTCGGGGGAGGTGTCAATTCACAACAGGCTTCAA
  ACGTCCCAGCACAGCCCCAAGTTGGGGGTCGAGAGGGGAGCGCCTGCACCATCACCACCAACATCACCAC
  CGCCGTTGCCGCCAGACCCACAGCCTAGCCCCCTGAGGGAGCAGCCCCCACCGCTGCCACTGCCACCACC
  CACACCTCCAGGCACCCTGGTACAGTGCCAGCAGATTGTCAAGGTCATTGTCCTGGACAAACCCTGCTTG
  GCCCGCATGGAGCCCCTGCTGAGCCAGGCTCTGTCCTGTTACGCCTCATCCTCCTCAGACTCCTGTGGCT
  CCACACCCTTGCGTGGTCCAGGCTCCCCAGTTAAGGTCATCCACCAGCCTCCACTGCCCCCGCCCCCGCC
  CCCATACAACCACCCTCATCAGTTCTGCCCCCCAGGCTCCATGCTGCTCCGGCGCCGTTACTCCAGCGGC
  TCCAGGAGCCTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224906 representing NM_001033354
  Red=Cloning site Green=Tags(s)
  
  MESLLENPVRAVLYLKELTAIVQNQQSLIHTQRQRIDELERRLDELSAENRSLWEHQQLLQAQPPPGLVP
  PPPSAPLPAPAVTAPAAAAAQEPLQDHGQLIPASPEPPLQHHGQLLAQPQPAPSSRVQTPQSPHQHPVAP
  GAIADKEKERPSSCCAAAGALLQHASPAALGKGVLSRRPENETVLHQFCCPAADTEQKPACSDLASQSDG
  SCAQAGGGMEDSVVAAVAAGRPSAHAPKAQAPELQQEEERPGAVGSPRAGPLRAASPGRQQPALATALCS
  HTPAASEYELSLDLKNKQIEMLEHKYGGHLVSRRAACTIQTAFRQYQLSKNFEKIRNSLLESRLPRRISL
  RKVRAPTAESLVAEKALLEGCGLLGLPLGRSPSLPPTFAGSLTELEDSFTEQVQSLAKSIDDALSTWSLK
  TMCSLQESGAYQLHQALHPSAGQPGLETEAAAREPESGPGSGDEAGGLPQGHSGTLMMAFRDVTVQIANQ
  NISVSSSTALSVANCLGAQTAQATAEPAAAQAEQEDTADQEVSEVPASEQMDPPSEDSEAAESRAQSAQE
  PAVAQAVVEEAVATEAEEEEEGAKQAGKGAEAEGGDNSEQLSSSSASTKSAKSSSEASAAASKEALQAVI
  LSLPRYHCENPASCRSPTLSTDTLRKRLYRIGLNLFNINPDKGIQFLISRGFIPDTPIGVAHFLLQRKGL
  SRQMIGEFLGNSKKQFNRDVLDCVVDEMDFSNMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCMCN
  PEVVQQFHNPDTIFILAFAIILLNTDMYSPNIKPDRKMMLEDFIRNLRGVDDGADIPRELVVGIYERIQQ
  KELKSNEDHVTYVTKVEKSIVGMKTVLSMPHRRLVCCSRLFEVTDVNKLQKQAAHQREVFLFNDLLVILK
  LCPKKKSSFTYTFCKAVGLLGMRFHLFENEYYSHGITLATPLSGSEKKQVLHFCALGSDEMQKFVEDLKE
  SIAEVTELEQIRIEWELEKQQGTKTLSVRSAGAQGDPQSKQGSPTAKREAMAGEKAAESSGEVSIHNRLQ
  TSQHSPKLGVERGAPAPSPPTSPPPLPPDPQPSPLREQPPPLPLPPPTPPGTLVQCQQIVKVIVLDKPCL
  ARMEPLLSQALSCYASSSSDSCGSTPLRGPGSPVKVIHQPPLPPPPPPYNHPHQFCPPGSMLLRRRYSSG
  SRSLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033354
• ORF Size: 3585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033354.1, NM_001033354.2, NM_001033354.3, NP_001028526.1
• RefSeq Size: 6861 bp
• RefSeq ORF: 3588 bp
• Locus ID: 243621
• Cytogenetics: 6 F1
• MW: 129.1 kDa