Dnm1l (NM_152816) Mouse Tagged ORF Clone

CAT#: MR225302

Dnm1l (Myc-DDK-tagged) - Mouse dynamin 1-like (Dnm1l), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm1l
• Synonyms: 6330417M19Rik; AI450666; Dlp1; Dnmlp1; Drp1; python
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225302 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC
  AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
  CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT
  TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGACCCTGCCACATGGAAAAACTCAAGACACC
  TTTCCAAAGGAGTTGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAAAAACAAGCTTTACACAGATTT
  TGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAGAATTTCAGGAAATAATAAGGGGGTAAGCCCT
  GAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTCAACCTGACACTTGTGGATTTACCGGGAATGA
  CCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGCTTCAGATCAGAGAACTTATTCTTCGGTTCAT
  CAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGCAAATACAGATATGGCAACATCAGAAGCACTC
  AAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACTCTAGCTGTAATCACTAAACTTGATCTCATGG
  ATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAGTCAAGCTTGGAATAATTGGAGT
  AGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCT
  TTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAATGGAACAAAGTATCTTGCTAGGACCCTGAATA
  GGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGCTGAAAACAAGAATAAATGTCTTAGCTGCTCA
  GTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGATGATAAAAGTGCTACTTTACTCCAGCTTATT
  ACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGAACCGCAAAGTACATTGAAACTTCTGAGCTAT
  GCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTTTCGGGCGAACCTTAGAATCTGTGGACCCACT
  AGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAGAAATGCTACTGGCCCCCGTCCTGCTTTATTT
  GTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAGATTAAGCGTCTAGAAGAGCCCAGCCTACGGT
  GTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTCAGCATTGTAGCAATTACAGCACACAGGAATT
  GTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGTAGTGACCTGTCTTCTTCGTAAAAGGTTGCCC
  GTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAGCTAGCGTATATCAACACAAAACACCCCGACT
  TTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGGAACAAAGAAGAAACAGGCTAGCAAGAGAGCT
  GCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGGTGGTGGGATTGGAGACGGTGGTCAGGAACCA
  ACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTGAAGAAAAATCAA
  AACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCCATGCTGTCAATTTGCTAGATGTGCCAGTTCC
  AGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTTATCAAATCATAT
  TTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCAAAGGCAGTAATGCATTTTTTGGTTAATCATG
  TGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGTATAAGTCATCCTTATTAGATGACCTTCTGAC
  TGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGCGGATATGCTGAAGGCATTACAAGGAGCCAGT
  CAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225302 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV
  SPEDKRKTTGEENDPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSP
  EPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEAL
  KISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYA
  FLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLI
  TKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALF
  VPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLP
  VTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAGSRDKVPSAGGGIGDGGQEP
  TTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSY
  FLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGAS
  QIIAEIRETHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152816
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152816.2, NM_152816.3, NP_690029.2
• RefSeq Size: 4073
• RefSeq ORF: 2139
• Locus ID: 74006
• MW: 79.5 kDa
• Gene Summary: This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]