Ebf1 (NM_007897) Mouse Tagged ORF Clone

CAT#: MR225312

Ebf1 (Myc-DDK-tagged) - Mouse early B-cell factor 1 (Ebf1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ebf1
• Synonyms: Ebf; O/E-1; OE-1; Olf-1; Olf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225312 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATCCAGGAAAGCATCCAACGGAGTGGAAGCAGTATGAAGGAAGAGCCGCTGGGCAGCGGCA
  TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACAGCGGCGCAGAGCGGGGT
  GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCTAACCTGCGGAAATCCAACTTCTTCCACTTC
  GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATTGAGAGGACTGCCTTTGTGGGGTTCGTGG
  AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAATGGGATCCACTACCGGCTCCAGCTCCTCTACAG
  CAATGGGATACGGACAGAACAGGATTTCTATGTGCGCCTCATCGACTCCATGACAAAACAAGCCATAGTG
  TATGAAGGCCAAGACAAGAACCCTGAAATGTGCCGAGTATTGCTCACACACGAGATCATGTGCAGCCGCT
  GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT
  CCTGAAGTTTTTCCTTAAATGCAACCAAAATTGCCTAAAGAATGCAGGAAACCCACGTGACATGCGGAGA
  TTCCAGGTCGTGGTGTCTACCACAGTCAACGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG
  TCCACAATAACTCCAAGCACGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT
  GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGCGCGACTGTG
  ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT
  TGATAACTCCTCATGCCATCCGAGTTCAGACACCTCCTCGGCACATCCCTGGTGTGGTGGAAGTCACACT
  GTCGTACAAGTCCAAGCAGTTCTGCAAAGGGACACCAGGCAGATTCATCTACACAGCACTCAATGAACCC
  ACCATCGACTACGGCTTCCAGAGGTTACAGAAGGTCATTCCTCGGCATCCTGGTGACCCAGAGCGCTTGC
  CAAAGGAAGTGATCCTTAAAAGAGCTGCAGATCTGGTTGAAGCCCTGTATGGGATGCCCCACAACAACCA
  GGAGATTATCCTGAAGAGAGCTGCCGACATTGCAGAGGCTCTGTACAGTGTCCCTCGGAACCACAACCAG
  CTCCCAGCCCTTGCTAACACTTCGGTCCATGCAGGGATGATGGGTGTGAACTCCTTCAGTGGACAACTGG
  CTGTGAATGTCTCGGAGGCATCACAAGCCACCAATCAAGGTTTCACCCGCAACTCAAGCAGCGTGTCACC
  ACATGGCTATGTGCCGAGCACCACCCCACAGCAAACCAACTATAACTCAGTCACCACAAGCATGAATGGC
  TACGGCTCAGCCGCCATGTCCAATTTGGGCGGCTCCCCAACCTTCCTCAATGGCTCAGCTGCCAACTCAC
  CCTATGCCATTGTGCCATCCAGCCCCACCATGGCCTCATCTACAAGCCTCCCCTCCAACTGCAGTAGCTC
  CTCTGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCAGTGAAACAGAAGAGTGCTTTCGCACCA
  GTTGTCAGACCCCAGACGTCCCCTCCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT
  CTGGCATGATTGTCCCTCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225312 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
  VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
  YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR
  FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATV
  IIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
  TIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQ
  LPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNG
  YGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAP
  VVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007897
• ORF Size: 1776 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007897.1, NM_007897.2, NM_007897.3, NP_031923.1
• RefSeq Size: 5203
• RefSeq ORF: 1776
• Locus ID: 13591
• MW: 64.5 kDa