Smarcb1 (NM_001161853) Mouse Tagged ORF Clone

CAT#: MR225556

Smarcb1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (Smarcb1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smarcb1
• Synonyms: AU020204; Baf47; Ini1; Snf5; SNF5/INI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225556 representing NM_001161853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGATGGCGTTGAGCAAGACCTTCGGGCAGAAGCCCGTCAAGTTTCAGCTGGAGGACGACGGGG
  AGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTGCGTATGTTCCGAGGTTCTCTGTACAAGAGATA
  CCCCTCACTCTGGCGGCGACTAGCCACTGTGGAAGAAAGGAAGAAAATAGTGGCATCGTCACATGATCAT
  GGATATACCACCCTGGCCACCAGCGTGACACTCCTGAAAGCCTCAGAGGTAGAAGAGATCCTGGATGGCA
  ATGACGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCGACCTACCTCAGGGAGCAGAAGGCCAA
  GAGGAACAGCCAGTGGGTCCCCACCCTGCCCAACAGCTCCCACCACCTGGATGCTGTGCCCTGTTCCACC
  ACCATCAACAGGAACCGCATGGGTCGGGACAAGAAGAGAACCTTCCCCTTGTGCTTTGATGACCACGACC
  CAGCTGTGATCCATGAGAATGCGTCACAGCCTGAGGTGCTGGTGCCCATCCGGCTCGACATGGAGATCGA
  CGGGCAGAAGCTGCGAGACGCTTTTACCTGGAACATGAATGAGAAGCTAATGACTCCTGAGATGTTTTCA
  GAAATACTTTGTGATGACCTGGATTTGAATCCACTGACTTTTGTGCCAGCTATTGCCTCTGCCATTCGAC
  AGCAGATTGAGTCCTACCCCACAGACAGCATCCTAGAGGATCAATCCGACCAGCGTGTCATCATCAAGCT
  GAACATCCACGTGGGGAACATCTCCCTGGTGGACCAGTTTGAGTGGGACATGTCAGAGAAAGAGAACTCC
  CCAGAGAAGTTTGCCCTGAAGCTGTGCTCAGAGCTGGGCTTGGGCGGGGAGTTTGTCACCACCATTGCAT
  ACAGCATCCGAGGACAGCTGAGCTGGCACCAGAAGACCTATGCCTTCAGTGAGAACCCACTTCCCACAGT
  GGAGATTGCCATCCGAAATACCGGAGATGCTGACCAGTGGTGCCCCCTGCTGGAGACACTGACTGATGCC
  GAGATGGAGAAAAAGATCCGGGATCAAGATAGGAACACAAGGCGAATGAGGCGTCTTGCCAACACTGCCC
  CAGCCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225556 representing NM_001161853
  Red=Cloning site Green=Tags(s)
  
  MMMMALSKTFGQKPVKFQLEDDGEFYMIGSEVGNYLRMFRGSLYKRYPSLWRRLATVEERKKIVASSHDH
  GYTTLATSVTLLKASEVEEILDGNDEKYKAVSISTEPPTYLREQKAKRNSQWVPTLPNSSHHLDAVPCST
  TINRNRMGRDKKRTFPLCFDDHDPAVIHENASQPEVLVPIRLDMEIDGQKLRDAFTWNMNEKLMTPEMFS
  EILCDDLDLNPLTFVPAIASAIRQQIESYPTDSILEDQSDQRVIIKLNIHVGNISLVDQFEWDMSEKENS
  PEKFALKLCSELGLGGEFVTTIAYSIRGQLSWHQKTYAFSENPLPTVEIAIRNTGDADQWCPLLETLTDA
  EMEKKIRDQDRNTRRMRRLANTAPAW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161853
• ORF Size: 1131 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161853.1, NP_001155325.1
• RefSeq Size: 1647
• RefSeq ORF: 1131
• Locus ID: 20587
• MW: 43.2 kDa