Sirt2 (NM_022432) Mouse Tagged ORF Clone

CAT#: MR225715

Sirt2 (Myc-DDK-tagged) - Mouse sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) (Sirt2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sirt2
• Synonyms: 5730427M03Rik; Sir2l; SIR2L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225715 representing NM_022432
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCCGGACCCCTCTGACCCTCTGGAGACCCAGGCAGGGAAGGTGCAGGAGGCTCAGGATTCAG
  ACTCGGACACTGAGGGAGGAGCCACTGGTGGAGAGGCAGAGATGGACTTCCTGAGGAATTTATTCACCCA
  GACCCTGGGCCTGGGTTCCCAAAAGGAGCGTCTTCTAGACGAGCTGACCCTCGAAGGAGTGACACGCTAC
  ATGCAGAGCGAGCGCTGCCGCAAGGTCATCTGTTTGGTGGGAGCCGGAATCTCCACGTCCGCGGGTATCC
  CTGACTTCCGCTCCCCATCCACTGGCCTCTATGCAAACCTGGAGAAGTACCACCTTCCTTACCCAGAGGC
  CATCTTTGAGATCAGCTACTTCAAGAAACATCCGGAACCCTTCTTTGCCCTTGCCAAGGAGCTCTATCCC
  GGGCAGTTCAAGCCAACCATCTGCCACTACTTCATCCGCCTGCTGAAGGAGAAGGGGCTGCTGCTGCGCT
  GCTACACGCAGAACATAGACACGCTGGAACGAGTGGCGGGGCTGGAGCCCCAGGACCTGGTGGAGGCCCA
  CGGCACCTTCTACACATCACACTGTGTCAACACCTCCTGCAGAAAAGAATACACGATGGGCTGGATGAAA
  GAGAAGATCTTCTCAGAAGCAACTCCCAGGTGTGAGCAGTGTCAGAGCGTGGTAAAGCCCGATATCGTGT
  TTTTCGGTGAGAACCTTCCACCGCGCTTCTTCTCCTGCATGCAGTCAGACTTCTCCAAGGTGGACCTCCT
  CATCATCATGGGCACCTCCCTGCAGGTGCAGCCCTTCGCCTCCCTCATCAGCAAGGCACCACTAGCCACC
  CCACGGCTGCTCATTAACAAGGAAAAGACAGGCCAGACGGACCCCTTCCTGGGCATGATGATGGGCCTGG
  GAGGTGGCATGGATTTTGACTCCAAGAAGGCTTACAGGGACGTGGCCTGGCTGGGTGACTGTGATCAAGG
  CTGCCTGGCTCTCGCTGACCTCCTCGGATGGAAGAAGGAACTGGAAGACCTTGTCCGGAGGGAGCATGCC
  AACATAGATGCCCAGTCAGGGTCACAGGCCCCCAACCCCAGCACTACCATCTCCCCTGGAAAGTCCCCAC
  CGCCTGCCAAGGAGGCGGCCAGGACCAAAGAGAAAGAGGAACAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225715 representing NM_022432
  Red=Cloning site Green=Tags(s)
  
  MAEPDPSDPLETQAGKVQEAQDSDSDTEGGATGGEAEMDFLRNLFTQTLGLGSQKERLLDELTLEGVTRY
  MQSERCRKVICLVGAGISTSAGIPDFRSPSTGLYANLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYP
  GQFKPTICHYFIRLLKEKGLLLRCYTQNIDTLERVAGLEPQDLVEAHGTFYTSHCVNTSCRKEYTMGWMK
  EKIFSEATPRCEQCQSVVKPDIVFFGENLPPRFFSCMQSDFSKVDLLIIMGTSLQVQPFASLISKAPLAT
  PRLLINKEKTGQTDPFLGMMMGLGGGMDFDSKKAYRDVAWLGDCDQGCLALADLLGWKKELEDLVRREHA
  NIDAQSGSQAPNPSTTISPGKSPPPAKEAARTKEKEEQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022432
• ORF Size: 1167 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022432.1, NM_022432.2, NM_022432.3, NM_022432.4, NP_071877.3
• RefSeq Size: 1863
• RefSeq ORF: 1170
• Locus ID: 64383
• MW: 43.7 kDa
• Gene Summary: Isoform 4: Deacetylates alpha-tubulin. [UniProtKB/Swiss-Prot Function]