Trim72 (NM_001079932) Mouse Tagged ORF Clone

CAT#: MR226036

Trim72 (Myc-DDK-tagged) - Mouse tripartite motif-containing 72 (Trim72)

Product Images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

• 

  Coomassie blue staining of purified Trim72 protein (Cat# TP526036). The protein was produced from HEK293T cells transfected with Trim72 cDNA clone (Cat# MR226036) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim72
• Synonyms: BC067209; MG53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226036 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCTGCACCCGGCCTTCTGCGTCAGGAACTGTCCTGCCCACTGTGCTTGCAGCTGTTCGATGCGC
  CAGTGACGGCTGAGTGTGGCCACAGTTTCTGCCGTGCCTGCCTGATCCGGGTGGCAGGGGAGCCTGCTGC
  GGACGGCACAGTTGCCTGTCCCTGTTGTCAGGCACCTACACGGCCGCAGGCTCTAAGCACTAACCTCCAG
  TTGTCACGCCTTGTGGAGGGTTTGGCGCAAGTGCCCCAAGGCCACTGCGAGGAACACCTGGATCCACTGA
  GCATCTACTGCGAGCAGGACCGCACACTTGTGTGTGGTGTGTGTGCCTCGCTCGGTTCTCACCGTGGTCA
  TCGTCTCCTGCCTGCCGCTGAAGCCCAAGCACGCCTCAAGACACAGCTTCCACAGCAGAAGATGCAGCTG
  CAGGAGGCATGCATGCGCAAGGAGAAGACTGTAGCGGTGCTGGAGCATCAGCTGGTGGAGGTGGAGGAGA
  CAGTGCGCCAGTTCCGGGGAGCTGTCGGGGAGCAGCTGGGGAAGATGCGGATGTTCCTGGCTGCCCTAGA
  AAGTTCTCTGGACCGTGAAGCAGAAAGGGTTCGGGGTGATGCTGGGGTTGCCTTGCGTCGGGAGCTGTCA
  AGCCTGAACTCTTACCTAGAGCAACTGAGGCAGATGGAGAAGGTGCTGGAGGAGGTGGCTGACAAGCCAC
  AGACAGAATTCCTCATGAAATTCTGCCTGGTAACCAGCAGGCTGCAGAAGATCCTGTCAGAGTCACCACC
  ACCGGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAGAAGATG
  TTCCGGGCTCTGATGCCAGCGCTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCCTGGTGG
  TGTCCTCCTCTGGTCGCCGAGTGGAGTGCTCAGACCAGAAGGCGCCGCCAGCGGGAGAAGACACGCGTCA
  GTTCGACAAGGCAGTAGCGGTGGTGGCGCAGCAGCTGCTGTCACAGGGCGAGCACTATTGGGAGGTGGAG
  GTGGGCGACAAACCACGCTGGGCCCTGGGAGTGATGGCGGCTGACGCTTCCCGCCGTGGCCGGCTGCACG
  CGGTGCCCTCACAGGGGCTGTGGCTGCTGGGTCTGCGCGATGGCAAGATCCTGGAGGCGCACGTGGAGGC
  CAAGGAGCCGCGGGCACTGCGCACCCCAGAGAGGCCTCCGGCGCGCATTGGCCTCTACCTAAGCTTCGCA
  GATGGCGTCCTGGCTTTCTATGATGCGAGCAACCCCGACGTACTTACGCCAATCTTTTCTTTCCACGAGC
  GTCTGCCCGGGCCGGTGTACCCCATCTTTGACGTGTGCTGGCACGACAAGGGCAAGAATGCCCAGCCCCT
  GCTGCTTGTGGGGCCGGAGCAGGAACAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226036 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAAPGLLRQELSCPLCLQLFDAPVTAECGHSFCRACLIRVAGEPAADGTVACPCCQAPTRPQALSTNLQ
  LSRLVEGLAQVPQGHCEEHLDPLSIYCEQDRTLVCGVCASLGSHRGHRLLPAAEAQARLKTQLPQQKMQL
  QEACMRKEKTVAVLEHQLVEVEETVRQFRGAVGEQLGKMRMFLAALESSLDREAERVRGDAGVALRRELS
  SLNSYLEQLRQMEKVLEEVADKPQTEFLMKFCLVTSRLQKILSESPPPARLDIQLPVISDDFKFQVWKKM
  FRALMPALEELTFDPSSAHPSLVVSSSGRRVECSDQKAPPAGEDTRQFDKAVAVVAQQLLSQGEHYWEVE
  VGDKPRWALGVMAADASRRGRLHAVPSQGLWLLGLRDGKILEAHVEAKEPRALRTPERPPARIGLYLSFA
  DGVLAFYDASNPDVLTPIFSFHERLPGPVYPIFDVCWHDKGKNAQPLLLVGPEQEQA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079932
• ORF Size: 1434 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079932.1, NM_001079932.2, NM_001079932.3, NP_001073401.1
• RefSeq Size: 2472
• RefSeq ORF: 1434
• Locus ID: 434246
• MW: 52.8 kDa