Ntrk2 (NM_001025074) Mouse Tagged ORF Clone

CAT#: MR226130

Ntrk2 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ntrk2
• Synonyms: GP145-TrkB/GP95-TrkB; Tkrb; trk-B; trkB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226130 representing NM_001025074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCTGGCTGAAGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTATGCCTGCTGGTCTTGG
  GCTTCTGGAGGGCCTCTCTCGCCTGCCCGACGTCCTGCAAATGCAGTTCCGCTAGGATTTGGTGTACTGA
  GCCTTCTCCAGGCATCGTGGCATTCCCGAGGTTGGAACCTAACAGCGTTGACCCGGAGAACATCACGGAA
  ATTCTCATTGCAAACCAGAAAAGGCTAGAAATCATCAATGAAGATGACGTTGAAGCTTACGTGGGGCTGA
  GAAACCTTACAATTGTGGATTCCGGCTTAAAGTTTGTGGCTTACAAAGCGTTTCTGAAAAACAGCAACCT
  GCGGCACATAAATTTCACACGAAACAAGCTGACGAGTTTGTCCAGGAGACATTTCCGCCACCTTGACTTG
  TCTGACCTGATCCTGACGGGTAATCCGTTCACGTGCTCCTGCGACATCATGTGGCTCAAGACTCTCCAGG
  AGACTAAATCCAGCCCCGACACTCAGGATTTGTACTGCCTCAATGAGAGCAGCAAGAACATGCCCCTGGC
  GAACCTGCAGATACCCAATTGTGGTCTGCCATCTGCACGTCTGGCTGCTCCTAACCTCACCGTGGAGGAA
  GGAAAGTCTGTGACCCTTTCCTGCAGTGTGGGGGGTGACCCACTCCCCACCTTGTACTGGGACGTTGGGA
  ATTTGGTTTCCAAGCACATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACGAACATTTCATC
  TGATGACAGTGGAAAGCAAATCTCTTGTGTGGCAGAAAACCTTGTAGGAGAAGATCAAGATTCTGTGAAC
  CTCACTGTGCATTTTGCGCCAACTATCACGTTTCTCGAGTCTCCAACCTCAGATCACCACTGGTGCATTC
  CATTCACTGTGAGAGGCAACCCCAAGCCTGCGCTTCAGTGGTTCTACAATGGGGCCATACTGAATGAGTC
  CAAGTACATCTGTACTAAGATCCACGTCACCAATCACACGGAGTACCATGGCTGCCTCCAGCTGGATAAC
  CCCACTCATATGAATAACGGAGACTACACCCTGATGGCCAAGAACGAGTATGGGAAGGATGAGAGACAGA
  TCTCCGCTCACTTCATGGGCCGGCCTGGAGTCGACTACGAGACAAACCCAAATTACCCTGAAGTCCTCTA
  TGAAGACTGGACCACGCCAACTGACATTGGGGATACTACGAACAAAAGTAATGAAATCCCCTCCACGGAT
  GTTGCTGACCAAAGCAATCGGGAGCATCTCTCGGTCTATGCCGTGGTGGTGATTGCATCTGTGGTGGGAT
  TCTGCCTGCTGGTGATGTTGCTCCTGCTCAAGTTGGCGAGACATTCCAAGTTTGGCATGAAAGGCCCAGC
  TTCGGTCATCAGCAACGACGATGACTCTGCCAGCCCCCTCCACCACATCTCCAATGGGAGTAACACTCCA
  TCTTCTTCGGAGGGCGGTCCCGACGCTGTCATTATTGGAATGACCAAGATTCCTGTTATTGAAAACCCCC
  AGTACTTTGGCATCACCAACAGTCAGCTCAAGCCAGACACATTTGTTCAGCATATCAAGAGACACAACAT
  CGTTCTGAAGAGGGAACTTGGGGAAGGAGCCTTCGGGAAAGTTTTCCTTGCCGAGTGCTACAACCTCTGC
  CCAGAGCAGGATAAGATCCTGGTGGCTGTGAAGACGCTGAAGGACGCCAGCGACAATGCACGCAAGGACT
  TTCATCGGGAAGCTGAGCTGCTGACCAACCTCCAGCACGAGCACATTGTCAAGTTCTACGGTGTCTGTGT
  GGAGGGCGACCCACTCATCATGGTCTTTGAGTACATGAAGCACGGGGACCTCAACAAGTTCCTTAGGGCA
  CACGGGCCCGACGCAGTGCTGATGGCAGAGGGTAACCCGCCCACAGAGCTGACGCAGTCGCAGATGCTGC
  ACATCGCTCAGCAAATCGCAGCAGGTATGGTCTACCTGGCGTCCCAACACTTTGTGCACCGTGACCTGGC
  CACCCGGAACTGCCTGGTGGGAGAGAACCTGCTGGTGAAAATTGGGGACTTTGGGATGTCCCGAGATGTG
  TACAGCACCGACTACTATCGGGTCGGTGGCCACACAATGTTGCCCATCCGATGGATGCCTCCAGAGAGCA
  TCATGTACAGGAAATTCACCACCGAGAGCGACGTCTGGAGCCTGGGCGTTGTGTTGTGGGAGATCTTCAC
  CTACGGCAAGCAGCCCTGGTATCAGCTATCGAACAATGAGGTGATAGAGTGCATCACCCAGGGAAGAGTC
  CTTCAGCGGCCTCGAACGTGTCCCCAGGAGGTGTATGAGCTCATGCTTGGATGCTGGCAGCGGGAACCAC
  ACACCCGGAAGAACATCAAGAGCATCCACACCCTCCTTCAGAACTTGGCCAAGGCATCTCCCGTCTACCT
  GGATATCCTAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226130 representing NM_001025074
  Red=Cloning site Green=Tags(s)
  
  MSPWLKWHGPAMARLWGLCLLVLGFWRASLACPTSCKCSSARIWCTEPSPGIVAFPRLEPNSVDPENITE
  ILIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAYKAFLKNSNLRHINFTRNKLTSLSRRHFRHLDL
  SDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSARLAAPNLTVEE
  GKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVN
  LTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDN
  PTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTD
  VADQSNREHLSVYAVVVIASVVGFCLLVMLLLLKLARHSKFGMKGPASVISNDDDSASPLHHISNGSNTP
  SSSEGGPDAVIIGMTKIPVIENPQYFGITNSQLKPDTFVQHIKRHNIVLKRELGEGAFGKVFLAECYNLC
  PEQDKILVAVKTLKDASDNARKDFHREAELLTNLQHEHIVKFYGVCVEGDPLIMVFEYMKHGDLNKFLRA
  HGPDAVLMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDV
  YSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSLGVVLWEIFTYGKQPWYQLSNNEVIECITQGRV
  LQRPRTCPQEVYELMLGCWQREPHTRKNIKSIHTLLQNLAKASPVYLDILG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025074
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025074.1, NM_001025074.2, NP_001020245.1
• RefSeq Size: 4614 bp
• RefSeq ORF: 2466 bp
• Locus ID: 18212
• Cytogenetics: 13 31.2 cM
• MW: 92.6 kDa