Slc1a2 (NM_001077515) Mouse Tagged ORF Clone

CAT#: MR226163

Slc1a2 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc1a2
• Synonyms: 1700091C19Rik; 2900019G14Rik; AI159670; Eaat2; GLT-1; GLT1; MGLT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226163 representing NM_001077515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGTGCCAACAATATGCCCAAGCAGGTAGAAGTGCGCATGCATGACAGCCACCTCAGCTCCGATG
  AGCCAAAGCACCGAAACCTGGGCATGCGCATGTGCGACAAGCTGGGGAAAAATCTCCTGCTCTCACTGAC
  TGTGTTTGGTGTCATCCTGGGAGCAGTGTGTGGCGGGCTGCTTCGCTTGGCATCGCCCATCCACCCTGAT
  GTGGTCATGTTGATAGCCTTCCCGGGGGACATACTCATGAGGATGCTGAAGATGCTCATCCTCCCTCTTA
  TCATCTCCAGTTTAATCACAGGGTTGTCAGGCCTGGATGCTAAAGCCAGCGGCCGCCTAGGCACGAGAGC
  TATGGTGTATTACATGTCCACGACCATCATTGCCGCCGTGCTGGGGGTCATCCTGGTGTTGGCCATCCAC
  CCAGGCAATCCCAAACTCAAGAAGCAGCTAGGGCCCGGGAAGAAGAACGACGAGGTGTCTAGCCTGGATG
  CCTTCCTGGATCTCATTAGAAATCTCTTCCCGGAGAACCTGGTGCAAGCCTGTTTCCAGCAGATTCAGAC
  AGTGACAAAGAAAGTTCTGGTGGCACCTCCATCTGAGGAGGCCAATACCACCAAGGCGGTCATCTCCATG
  TTGAATGAAACCATGAACGAGGCCCCTGAAGAAACTAAGATCGTTATCAAGAAGGGCCTGGAGTTCAAGG
  ACGGGATGAACGTCTTAGGTCTGATCGGATTCTTTATTGCTTTCGGCATTGCCATGGGGAAGATGGGTGA
  ACAGGCCAAGCTGATGGTGGAGTTCTTCAACATTCTGAATGAGATCGTGATGAAGTTAGTGATCATGATC
  ATGTGGTACTCCCCTCTGGGTATCGCCTGCTTGATTTGTGGGAAGATCATCGCCATCAAGGACTTAGAAG
  TGGTTGCTAGGCAGCTGGGGATGTACATGATCACCGTGATCGTGGGCCTCATCATTCACGGGGGCATCTT
  TCTCCCCTTGATTTACTTTGTAGTGACCAGAAAAAATCCATTCTCCTTTTTTGCTGGCATATTCCAAGCC
  TGGATCACTGCTCTGGGAACTGCTTCCAGTGCTGGAACTTTGCCTGTTACCTTCCGTTGCTTGGAAGATA
  ATCTAGGGATTGACAAGCGTGTGACCAGATTCGTCCTCCCAGTCGGAGCAACCATTAACATGGATGGCAC
  AGCCCTTTACGAGGCTGTGGCAGCCATCTTCATAGCCCAAATGAATGGGGTCATCTTGGATGGAGGTCAG
  ATTGTGACTGTAAGCCTTACAGCCACCCTGGCGAGCATTGGTGCAGCCAGTATTCCCAGCGCCGGGCTGG
  TCACCATGCTCCTCATTCTCACAGCTGTGGGCCTGCCAACGGAGGATATCAGTCTGCTGGTGGCGGTGGA
  CTGGCTGCTGGATAGAATGAGAACTTCAGTCAATGTGGTGGGCGATTCTTTTGGGGCTGGGATTGTCTAT
  CACCTTTCCAAGTCTGAGCTGGACACCATTGACTCCCAACACCGAATGCAGGAAGACATCGAAATGACCA
  AGACGCAGTCCATTTACGACGACAAGAACCACAGGGAAAGCAACTCTAATCAGTGTGTCTATGCCGCACA
  CAACTCTGTCGTAATAGATGAGTGCAAGGTAACTCTGGCGGCCAATGGAAAGTCAGCTGACTGCAGTGTT
  GAGGAAGAACCTTGGAAACGTGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226163 representing NM_001077515
  Red=Cloning site Green=Tags(s)
  
  MVSANNMPKQVEVRMHDSHLSSDEPKHRNLGMRMCDKLGKNLLLSLTVFGVILGAVCGGLLRLASPIHPD
  VVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIH
  PGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPSEEANTTKAVISM
  LNETMNEAPEETKIVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGEQAKLMVEFFNILNEIVMKLVIMI
  MWYSPLGIACLICGKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQA
  WITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVILDGGQ
  IVTVSLTATLASIGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVY
  HLSKSELDTIDSQHRMQEDIEMTKTQSIYDDKNHRESNSNQCVYAAHNSVVIDECKVTLAANGKSADCSV
  EEEPWKREK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077515
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077515.1, NM_001077515.2, NP_001070983.1
• RefSeq Size: 10998
• RefSeq ORF: 1710
• Locus ID: 20511
• MW: 62.2 kDa
• Gene Summary: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. [UniProtKB/Swiss-Prot Function]