Dclk2 (NM_001195496) Mouse Tagged ORF Clone

CAT#: MR226382

Dclk2 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 2 (Dclk2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclk2
• Synonyms: 6330415M09Rik; AU044875; CL2; Click-II; CLICK2; Dcamkl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226382 representing NM_001195496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACAAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCAC
  GGAGAGGGGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG
  CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG
  GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTATTTCAAAGGCCTGGTCTTTGCCATCTCCAACG
  ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCACTGTCTGACAATGTGAACCTGCC
  CCAGGGCGTCCGCACCATCTACACCATAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
  GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTTCGTAAAGTTGATTACACCAAAAACGTTAATCCGA
  ACTGGTCTGTGAACATCAAGGGCGGAACCACCCGCACCCTGGCCGTGGCCTCTGCGAAGAGTGAGGTGAA
  AGAAAGCAAAGACTTCATTAAACCCAAGTTAGTGACTGTGATTCGAAGCGGAGTGAAGCCTAGAAAGGCC
  GTGCGGATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCTTAACAGATATCACCGAAGCCA
  TTAAACTAGACTCAGGCGTGGTCAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA
  CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT
  GTCCTGGATCATAGCGAATGCCGTGTCCTGAAATCGTCCTACTCTCGAGCCTCAGCTGCGAAGTATTCTG
  GATCCAGAAGCCCAGGGTTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG
  TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGTAGCCAGCTTTCCACTCCGAAGTCCACC
  AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGATTGAAGATTTCTGCTCAGGGCAGAT
  CTTCTTCCAACGTAAACGGTGGGCCTGAACTTGACCGTTGCCTGAGCCCTGAAGGTGTGAATGGAAACCG
  GTGCTCCGAGTCGTTCCCCCTTCTGGAGAAATACAGAATAGGGAAGGTCATCGGGGACGGCAACTTCGCG
  GTAGTTAAGGAGTGCGTGGACAGGTACACTGGAAAAGAGTTTGCATTAAAGATTATAGACAAAGCCAAAT
  GCTGTGGAAAGGAGCATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGAGTGAAGCACCCCAACATCAT
  CATGTTGGTTGAAGAGATGGAAACAGCAACTGACCTCTTTCTAGTGATGGAACTGGTCAAAGGTGGAGAT
  CTCTTTGATGCGATTACCTCTTCAACCAAGTACACTGAGAGAGATGGAAGCGCCATGGTGTACAACCTAG
  CCAATGCCCTCCGGTACCTGCACAGCCTCAGCATCGTCCACAGGGACATCAAGCCTGAGAATCTGCTGGT
  GTGCGAATACCCAGATGGAACCAAGTCTTTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAAGGC
  CCGTTGTACACGGTCTGTGGCACGCCAACTTATGTGGCACCAGAGATCATAGCTGAAACAGGTTATGGCC
  TGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACATACATACTTCTCTGTGGATTCCCACCATTCCGGAG
  TGAGAACAATCTCCAGGAAGATCTCTTTGACCAGATCTTGGCTGGAAAGCTGGAATTCCCAGCCCCCTAC
  TGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTCGCT
  GTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAACAATATGCAAGC
  CGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACCGGG
  GTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGATTTTCTGCAGCAAGCTCTGTCAAGACA
  GCAGCAGACCATCCAGGGAGCAGACCTCGCCAGTCCCTCCCTCAGCCCAGGAGGCCCCTCCCCCACTGGA
  GTCTCCCAGGCCCCCTGGTCCTCCAGCCACCTCTGGCTGTGATCTGGCAGGGACCTGGCGCCGCCACCGA
  GAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226382 representing NM_001195496
  Red=Cloning site Green=Tags(s)
  
  MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK
  AKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLE
  GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA
  VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF
  VLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVKRAGHSSAYSTAKSPVNGTPSSQLSTPKST
  KSSSSSPTSPGSFRGLKISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEKYRIGKVIGDGNFA
  VVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGD
  LFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEG
  PLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPY
  WDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTG
  VSVIMNTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHR
  D
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195496
• ORF Size: 2313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001195496.1, NP_001182425.1
• RefSeq Size: 4092
• RefSeq ORF: 2316
• Locus ID: 70762
• MW: 84.9 kDa
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]