Myh7 (NM_080728) Mouse Tagged ORF Clone

CAT#: MR226406

Myh7 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myh7
• Synonyms: B-MHC; beta-MHC; betaMHC; MYH-beta/slow; Myhc-b; myHC-beta; MyHC-I; myHC-slow; Myhcb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226406 representing NM_080728
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGCAGAGATGGCTGCATTTGGGGCTGCAGCCCCCTTCCTGCGGAAGTCTGAGAAGGAGAGGC
  TGGAGGCACAGACCAGGCCCTTTGACCTCAAGAAAGATGTTTTTGTGCCCGATGACAAAGAAGAGTTTGT
  CAAGGCCAAGATCGTGTCCCGAGAGGGTGGCAAAGTCACTGCTGAGACGGAGAATGGCAAGACGGTGACT
  GTGAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA
  CCTTCCTGCATGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCTTCCTGGATGATCTATACCTA
  CTCGGGCCTCTTCTGCGTCACCGTCAACCCCTACAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCT
  GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT
  ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCTGGGAAGACTGTCAA
  CACTAAGAGGGTCATCCAATATTTTGCTGTTATTGCCGCCATTGGGGACCGCAGCAAGAAGGACCAGACC
  CCAGGCAAGGGTACCCTGGAAGATCAAATCATCCAAGCCAACCCTGCTCTGGAGGCCTTTGGCAATGCCA
  AGACAGTTCGGAATGACAACTCCTCTCGATTTGGGAAATTCATCCGAATCCATTTTGGGGCAACAGGAAA
  GTTGGCATCTGCAGACATAGAGACCTACCTTCTGGAAAAATCCAGGGTTATTTTCCAGCTGAAAGCAGAA
  AGAGATTATCACATTTTCTACCAAATCCTGTCTAATAAAAAGCCTGAGCTTCTAGACATGCTGCTGATCA
  CCAACAACCCCTACGATTATGCGTTCATCTCCCAAGGAGAGACGACTGTGGCCTCCATTGATGACTCTGA
  AGAGCTCATGGCCACGGATAGCGCCTTTGACGTGCTGGGCTTCACTCCAGAAGAGAAGAACTCCATTTAC
  AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGG
  AACCAGACGGCACTGAAGAGGCTGACAAATCAGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTTAA
  GGGGCTGTGCCATCCTAGAGTCAAAGTGGGCAACGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTG
  TCATACGCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCA
  ATGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGAGAT
  CTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAAC
  CACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGACCTTCATAGACTTCG
  GCATGGACTTGCAGGCCTGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTTGAGGAGGA
  GTGCATGTTCCCCAAGGCCACAGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAGTCC
  AACAACTTCCAGAAGCCTCGAAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCTTTGGTCCACTATGCTG
  GCACTGTGGACTACAATATCCTGGGCTGGCTACAGAAGAACAAGGACCCACTCAATGAGACGGTGGTGGG
  TTTGTACCAGAAGTCTTCCCTCAAGCTGCTCAGCAATCTATTTGCCAACTATGCTGGAGCTGATGCCCCG
  GCGGACAAAGGCAAAGGCAAGGCAAAGAAAGGCTCATCCTTTCAGACCGTGTCTGCTCTGCACAGAGAAA
  ATCTGAACAAACTTATGACAAACTTGCGCTCCACGCACCCTCACTTTGTACGCTGCATCATCCCCAATGA
  GACAAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTGCTGGAG
  GGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGTATCGCA
  TCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACTGCTGGG
  CTCCCTGGACATTGACCACAACCAATACAAGTTTGGTCACACCAAGGTGTTCTTCAAGGCGGGCCTGCTG
  GGGCTGCTGGAGGAGATGCGTGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCCCAGTCCCGAG
  GTGTGCTCTCCAGAATGGAGTTCAAGAAGCTGCTGGAGCGCAGAGACTCCCTGCTGATTATCCAGTGGAA
  CATTAGGGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG
  AAGAGCGCGGAGACGGAGAAGGAGATGGCCACCATGAAGGAGGAGTTTGGGCGAGTCAAAGATGCACTAG
  AGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT
  GCAGCTCCAAGTGCAGGCGGAACAAGACAACTTGGCGGATGCAGAGGAGCGCTGTGACCAGCTGATCAAG
  AACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGATGAATG
  CCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGCGGGATATCGATGACCT
  GGAGCTGACGCTGGCCAAGGTGGAGAAGGAAAAGCATGCAACAGAGAACAAGGTGAAAAACCTGACAGAG
  GAGATGGCTGGTTTGGATGAGATCATTGTCAAGCTGACAAAGGAGAAGAAAGCTCTGCAAGAGGCCCACC
  AGCAGGCTCTGGATGACCTGCAGGCTGAGGAAGACAAGGTCAATACTCTGACCAAGGCCAAGGTCAAGCT
  GGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAGGTGCGCATGGACCTAGAGCGA
  GCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACGCAGGAGAGCATCATGGACCTGGAGAATGACAAGC
  AGCAGTTGGATGAGCGACTCAAAAAGAAGGACTTTGAGTTAAATGCACTCAATGCCAGGATTGAGGATGA
  GCAAGCCCTGGGCAGTCAGCTGCAGAAGAAGCTCAAGGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG
  GAGCTGGAGGCCGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGGAGCTGG
  AGGAGATCAGTGAAAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAACAAGAAGCG
  CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACGGCGGCG
  GCCCTGCGCAAGAAGCATGCCGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGCGGGTGA
  AGCAGAAGCTGGAGAAAGAGAAAAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCA
  GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGG
  AGCAAGGCCGAGGAGACGCAGCGTTCTGTCAATGACCTCACCAGCCAGCGGGCCAAGCTGCAGACAGAGA
  ATGGGGAGCTGTCCCGGCAGCTGGACGAGAAGGAGGCTCTGATCTCTCAGCTAACCCGAGGCAAGCTCAC
  ATATACACAGCAGCTGGAGGACCTCAAGAGGCAACTGGAGGAGGAGGTCAAGGCCAAGAACGCGCTGGCC
  CACGCACTGCAGTCAGCACGGCATGATTGTGACCTGCTGAGGGAACAGTATGAGGAGGAGACAGAGGCCA
  AGGCTGAGCTACAGCGAGTCCTGTCCAAGGCCAATTCAGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC
  GGATGCCATACAGAGGACAGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAGGATGCA
  GAGGAGGCAGTGGAGGCTGTCAATGCCAAGTGTTCCTCTCTGGAGAAGACCAAGCACAGGCTGCAGAATG
  AGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAAGCAGAG
  GAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAGTCTTCC
  CAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTCTGGAGC
  ACCTAGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAGCAGCTGGG
  CTCCACGGGGAAGAGCATCCATGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCCGAGAAGCTGGAGCTG
  CAGTCGGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGCGCCCAGCTAG
  AGTTCAACCAGATCAAGGCAGAGATTGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCAGGCCAA
  GCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGCAATGAG
  GCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCATGCCAACC
  GTATGGCTGCTGAGGCCCAGAAACAAGTGAAGAGCCTCCAGAGTCTGCTGAAGGACACTCAAATCCAGCT
  GGATGATGCTGTCCGTGCCAATGACGACCTGAAAGAGAACATCGCCATCGTGGAACGGCGCAACAACCTG
  CTGCAGGCGGAGCTGGAGGAGCTTCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGGCAGAGC
  AGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAACCAGAA
  GAAGAAGATGGATGCAGACCTATCCCAGCTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGTAGGAAC
  GCAGAGGAGAAGGCCAAGAAGGCTATCACAGATGCCGCCATGATGGCTGAGGAGCTGAAGAAGGAGCAGG
  ACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCACCGTCT
  GGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGGGTCCGG
  GAGCTGGAGAATGAGCTGGAGGCTGAGCAAAAGCGCAATGCAGAGTCAGTGAAGGGCATGAGGAAGAGTG
  AGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAGGAAGAACCTACTGCGGCTGCAGGACCT
  GGTGGACAAGCTGCAGCTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAGGCCAAC
  ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACATCGCCG
  AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGGGCCTGAATGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226406 representing NM_080728
  Red=Cloning site Green=Tags(s)
  
  MADAEMAAFGAAAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVT
  VKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVA
  AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQT
  PGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAE
  RDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIY
  KLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQV
  SYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFN
  HHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKS
  NNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAP
  ADKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLE
  GIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLL
  GLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLL
  KSAETEKEMATMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIK
  NKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTE
  EMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLER
  AKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEE
  ELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAA
  ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHR
  SKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALA
  HALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDA
  EEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESS
  QKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLEL
  QSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNE
  ALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNL
  LQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRN
  AEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVR
  ELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQAN
  TNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKGLNEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080728
• ORF Size: 5805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 6054
• RefSeq ORF: 5808
• Locus ID: 140781
• MW: 222.9 kDa