Dclk1 (NM_001195539) Mouse Tagged ORF Clone

CAT#: MR226415

Dclk1 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 6

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclk1
• Synonyms: 1700113D08Rik; 2810480F11Rik; AI836758; Click-I; Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226415 representing NM_001195539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAGCTCATAGAAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCACGCTCGGGCAAGTCAC
  CAAGTCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGCGGCTCCTCGAC
  TTCACTTTCATCCACTAAAGTTTGCAGCTCAATGGATGAGAATGATGGCCCTGGGGAAGGTGATGAGCTT
  GGGAGAAGGCACAGCTTGCAGAGGGGATGGAGGAGGGAAGAGTCTGAGGAAGGCTTCCAGATTCCTGCCA
  CAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGACGGAAATTTTGCTGTTGTCAAGGAATGTAT
  AGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATCATCAAGAAAAGCAAATGCCGAGGCAAAGAGCAC
  ATGATCCAGAACGAGGTCTCCATCCTACGGAGGGTGAAGCACCCCAACATTGTCCTCCTGATTGAAGAGA
  TGGATGTGCCGACTGAACTGTATCTTGTAATGGAATTAGTGAAGGGTGGAGACCTTTTCGATGCCATCAC
  CTCCACTAGCAAATACACAGAGAGAGATGCCAGCGGGATGCTGTACAACCTGGCCAGCGCCATCAAATAC
  CTGCACAGCCTGAACATCGTCCACCGTGACATCAAGCCAGAGAATCTGCTGGTGTATGAGCACCAGGATG
  GCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCGACGGCCCCCTGTACACAGTCTG
  TGGCACCCCAACATATGTGGCTCCAGAAATCATTGCAGAGACTGGATATGGCCTCAAGGTGGACATCTGG
  GCAGCTGGCGTGATCACTTATATCCTGCTGTGTGGCTTCCCTCCGTTCCGTGGAAGTGGGGATGACCAGG
  AGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTCCGTATTGGGACAATGTGTCAGA
  TTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCAGAGATTTTCAGCCGTGCAGGTC
  CTTGAGCATCCCTGGGTTAATGATGATGGTCTCCCAGAAAATGAGCATCAGCTGTCAGTAGCTGGCAAAA
  TCAAGAAGCATTTCAACACAGGCCCCAAGCCGAGCAGCACTGCAGCAGGAGTTTCTGTAATAGCAACCAC
  CGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGATGTGAGGAGCCGGTACAAGGCG
  CAGCCAGCTCCACCGGAATTGAACTCGGAATCGGAGGACTACTCCCCCAGCTCCTCTGAGACTGTTCGCT
  CCCCCAATTCGCCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226415 representing NM_001195539
  Red=Cloning site Green=Tags(s)
  
  MLELIEVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRISQHGGSSTSLSSTKVCSSMDENDGPGEGDEL
  GRRHSLQRGWRREESEEGFQIPATITERYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKCRGKEH
  MIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKY
  LHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIW
  AAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQV
  LEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPSSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKA
  QPAPPELNSESEDYSPSSSETVRSPNSPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195539
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001195539.1, NP_001182468.1
• RefSeq Size: 7004
• RefSeq ORF: 1350
• Locus ID: 13175
• MW: 50.1 kDa
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010]