Dhx30 (NM_133347) Mouse Tagged ORF Clone

CAT#: MR226488

Dhx30 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhx30
• Synonyms: 2810477H02Rik; C130058C04Rik; Ddx30; HELG; Ret-CoR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226488 representing NM_133347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTCCTGTCTGTAATTCCAGCACTTGGCAGCCCAAGGATTCCAGCTTTCTCTCCTGGCCAGAAA
  TGTTCAGCTTGGACTCATTCAGAAAAGATCGGACCCAGCACAGGCAGCGTCAATGCAAGCTTCCCCCACC
  CCGTCTTCCACCCATGTGTGTCAACCCTGCCCCTGGAGGGACCATCACTCGAGCTTCTAGGGACCTATTA
  AAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCCTTGGCATCTCACATGCAA
  AAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCACCCTGCACATAAAGTGGCC
  CAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGTCAGGCTGCAGCAGCTGCC
  TGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTGATGCAGCTAAATACCGAG
  TGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACCCACCATGCCTCCAACTTC
  CTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTATCCCGATCCTTAGGCCGA
  GAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGACAGAGTTTCTGTCTATGA
  CCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGAAATGACAGATGATGACAG
  TGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAAGTGATTCAGATTGCAACC
  TCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGACCAAGCTGGCTACACTCA
  CTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGCTGAGGCTGAGAATAAGGC
  AGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAACAATGAGCCGCTTACCCAT
  GCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGCCATGTACCATCCAGGTGC
  CTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGACAGCTCATGGATTTCCCC
  AGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGGCCCTTGAGTGACCCTATC
  ACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCCAGAGCCTGCTAGAGCTGT
  GGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCCTCATCGGGACACTATCCT
  CAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGTGGGAAGACCACACGTATC
  CCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCAATGTGATCATCACACAAC
  CTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGGCCCCTCCTTGCGCCGGAA
  TGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCGCTGCTCTTCTGCACTGTG
  GGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCCATGTCATTGTGGATGAGG
  TCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCTGCAGCGGCTCAACCCAGC
  TCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGATACTTTGGGGGCTGCCCT
  GTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGGACATCTTGGCCAAGCTGG
  GTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATGTGCACTTGATTTGGACCT
  TGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATCCTGTGCTTTCTACCTGGC
  TGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGCATGAAAGCAAGTACCTCA
  TCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCAACAGCCTCCACTTGGGGT
  ACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAACGACATTGTACATGTCGTG
  GACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACTGTGTGGG
  TGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTCAGGTTTTGCCTACCACTT
  GTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGAG
  AACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGTTCCTCTCTAAGGCTGTGG
  ACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGATTGGGGTGCTGGACCAGCG
  GGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCCCGACTGGCCAAGGCCATA
  GTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCCT
  TCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCTGAGCCATGACAGTGGCAG
  TGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGCTGGCAGGACCGTACCTCC
  AGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCATCCACGGGCTCATCAAGC
  AGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTGCACACTGCCCTCTGCTCA
  GTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCTGGCCTCTACCCCAACCTC
  ATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACAGTGTCACTTACAGGACCA
  AATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCGGTTACGGAGCCGATGGCT
  GACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCCTCCCAGGTGCACCCACTA
  GCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTCGGGCCACCATCTCATTGA
  GTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCTAAGGGAGTTTCGTCGAGC
  CCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCTCTTAGTGTGCAGCAAGAA
  CACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCAGCTTTGATATGCGCAAGA
  CAGCTGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226488 representing NM_133347
  Red=Cloning site Green=Tags(s)
  
  MVTPVCNSSTWQPKDSSFLSWPEMFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLL
  KEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAA
  CQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGR
  EEEEDEEEELEEGTIDVTEFLSMTQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIAT
  SSSTAKNLMQFHTVGTKTKLATLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTH
  AMYNLASLRELGETQRRPCTIQVPEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPI
  TGKPYMPLSEAEEVRLSQSLLELWRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRI
  PQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTV
  GILLRKLQSNPSLEGVSHVIVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCP
  VIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPG
  WQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVV
  DSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLE
  NLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAI
  VLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTS
  RENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNL
  IQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPL
  AVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQE
  HGQLLALLAELLRGPCGSFDMRKTADD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133347
• ORF Size: 3651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133347.1, NM_133347.2, NP_579925.1
• RefSeq Size: 3903
• RefSeq ORF: 3654
• Locus ID: 72831
• MW: 137.1 kDa