Ehmt1 (NM_001109686) Mouse Tagged ORF Clone

CAT#: MR226600

Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehmt1
• Synonyms: 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226600 representing NM_001109686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA
  CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC
  TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA
  CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG
  AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA
  GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT
  CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA
  GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC
  AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG
  TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG
  ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC
  GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCAGCTGCAGTATCTCGGAAGAAA
  AAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAGTATTAAAACAGAGGACGGTGA
  TTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGAGAAAGCCTTAGATGATAGTGC
  CCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGACTCCGATGAGCTGGAGGATGAC
  GAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTAGGACTTCTAAAGAGAGCATGT
  CTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACAGGAGTCTCCCGACACAGGGGA
  GGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATCAAGAAGAAATTTCTCAAGAGG
  AGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGCGGAGAAGTAGAAAGAAGCCGA
  GCAGCATGCTTGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCCTCCAGGAAGTGCCTCTCTG
  CAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAACAACCAGTGCATGGCCACT
  GAGAGCGTGGATCACGAATTGGGCCGGTGTACAAACAGCGTGGTCAAGTATGAGCTGATGCGCCCATCCA
  ACAAAGCACCGCTCTTGGTGTTATGTGAAGACCATCGGGGTCGCATGGTGAAGCACCAGTGCTGTCCTGG
  CTGCGGCTACTTCTGCACGGCGGGTAACTTCATGGAATGCCAGCCTGAGAGCAGCATCTCTCATCGTTTC
  CATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGTGGGGAAGAAGCTTCCAAGG
  CCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAGCCCCTGGACAGGAGAAGAG
  CCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGCCCCAGAGGATGAAAGATCG
  CAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCTGGGCTCGTGAGGCCGACAT
  CTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCGCTCTAGACTCTGAAAAACC
  CAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGGTGAGCTGCAGAAGGTGCTT
  CTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGTAAGCGTTCCCCATTACATG
  CTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGGGTGCCAATATTGACACTTG
  CTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTTGGATGCAGTGAAGTACCTC
  ATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGTTTGCATTTGGCTGCCAAGA
  AAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATGTCAACTGCCAGGATGACGG
  TGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGTGAAGCTGCTGCTGTCTAAG
  GGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGGGCAGCATTTTCAGGCTGTG
  TGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGAATATCCATGGAGACTCACC
  CCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCTTTCTCGGGATTCAGATGTT
  ACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGTTCGCAGGTGTGGAGTGCAT
  TGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTGTTGAGAAGACGGTGAGCAG
  GGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGTGGACAGTGAGCTGTGTCCT
  ACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATTGACAGGAACATCACTCATT
  TGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTGGCCAGCTGAGCATGCGCTG
  CTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACCACCCTTGATCTTCGAGTGC
  AATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAATGGTCTCAGGGCAAGGCTGC
  AGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATATCCCACTGGGCACCTTTGT
  CTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGAAGAGGACTCTTACCTCTTT
  GATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTATGGGAATGTCAGCCGGTTCA
  TAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCACACCAGGACCTGCGGTTTCC
  CAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGGGTTCGACTACGGGGAGCGC
  TTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAGTGTCGGCACTCAAGCGCAG
  CCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCCTTCCAGATACCAGCTCTGC
  AGCCGCTGCTGACCCCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226600 representing NM_001109686
  Red=Cloning site Green=Tags(s)
  
  MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNA
  PKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRT
  PNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPK
  SILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTAAAVSRKK
  KRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDEDSDELEDD
  EDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSIKKKFLKR
  RGKTDSPWIKPARKRRRRSRKKPSSMLGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMAT
  ESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRF
  HKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAPEDERS
  QSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVL
  LMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLDAVKYL
  IKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVKLLLSK
  GSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDV
  TLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVDSELCP
  TNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFEC
  NHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREEDSYLF
  DLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGFDYGER
  FWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109686
• ORF Size: 3729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001109686.1, NM_001109686.2, NP_001103156.1
• RefSeq Size: 4932
• RefSeq ORF: 3732
• Locus ID: 77683
• MW: 136.9 kDa