Zap70 (NM_009539) Mouse Tagged ORF Clone

CAT#: MR226660

Zap70 (Myc-DDK-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zap70
• Synonyms: mrtle; mur; Srk; ZAP-70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226660 representing NM_009539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGATCCCGCGGCGCACCTGCCATTCTTCTATGGCAGCATCTCGCGGGCTGAGGCCGAGGAGCACC
  TGAAGCTGGCAGGCATGGCCGACGGGCTGTTCCTCCTGCGCCAGTGTTTGCGCTCCCTGGGCGGCTACGT
  GCTGTCGTTGGTGCACGACGTGCGCTTCCACCATTTCCCCATCGAGCGCCAACTCAACGGCACGTACGCC
  ATCGCGGGCGGGAAGGCGCACTGCGGCCCGGCCGAGCTCTGCCAGTTCTACTCTCAGGACCCCGACGGGC
  TGCCCTGCAACCTGCGTAAGCCGTGTAACCGGCCGCCCGGACTGGAGCCACAGCCCGGGGTCTTCGACTG
  CCTGCGTGATGCTATGGTGCGCGACTACGTGCGCCAGACCTGGAAGCTGGAGGGCGATGCACTAGAGCAG
  GCCATCATCAGCCAGGCCCCACAGGTGGAGAAGCTCATTGCTACCACAGCCCACGAGCGAATGCCCTGGT
  ATCACAGCAGCCTGACTCGTGAGGAGGCCGAGCGCAAACTCTATTCCGGCCAGCAGACCGACGGCAAGTT
  CCTGCTGAGGCCCCGGAAGGAGCAGGGCACATATGCACTGTCCCTGGTCTATGGGAAAACTGTATACCAC
  TATCTCATCAGCCAGGACAAGGCTGGCAAGTACTGCATCCCCGAAGGCACCAAGTTTGACACGCTCTGGC
  AGCTGGTGGAGTACCTGAAGCTGAAGGCGGATGGACTAATTTACCGCTTGAAGGAGGTCTGTCCCAACAG
  CAGCGCCAGCGCGGCAGTTGCTGCACCCACCCTCCCAGCCCACCCATCCACATTCACTCAGCCTCAGAGA
  CGAGTGGACACCCTGAACTCGGACGGATACACCCCTGAACCAGCACGCTTAGCGTCATCGACAGACAAGC
  CCCGGCCGATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGACAA
  GAAGCTCTTCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCTCC
  GTGCGCCAGGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCA
  CAGAGAAGGCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTACAT
  CGTGCGGCTCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGCCC
  CTGCACAAGTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCAGG
  TGGCCATGGGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTTCT
  ACTGGTCAATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACAGC
  TATTACACAGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGA
  AGTTCTCCAGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAA
  GCCCTACAAGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTCCG
  CCGGAGTGTCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCCCG
  ACTTCCTGACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCCCC
  ACAGTGTGAACAGGTGGCCGAGGCTGCATGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226660 representing NM_009539
  Red=Cloning site Green=Tags(s)
  
  MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYA
  IAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQ
  AIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYH
  YLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQR
  RVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGS
  VRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGP
  LHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDS
  YYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECP
  PECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009539
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009539.1, NM_009539.2, NM_009539.3, NP_033565.2
• RefSeq Size: 2180
• RefSeq ORF: 1857
• Locus ID: 22637
• MW: 70.6 kDa
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]