Col1a1 (NM_007742) Mouse Tagged ORF Clone
CAT#: MR226667
- TrueORF®
Col1a1 (Myc-DDK-tagged) - Mouse collagen, type I, alpha 1 (Col1a1)
"NM_007742" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Col1a1 |
Synonyms | Col1a-1; Cola-1; Cola1; Mov-13; Mov13 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226667 representing NM_007742
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTTTGTGGACCTCCGGCTCCTGCTCCTCTTAGGGGCCACTGCCCTCCTGACGCATGGCCAAG AAGACATCCCTGAAGTCAGCTGCATACACAATGGCCTAAGGGTCCCCAATGGTGAGACGTGGAAACCCGA GGTATGCTTGATCTGTATCTGCCACAATGGCACGGCTGTGTGCGATGACGTGCAATGCAATGAAGAACTG GACTGTCCCAACCCCCAAAGACGGGAGGGCGAGTGCTGTGCTTTCTGCCCGGAAGAATACGTATCACCAA ACTCAGAAGATGTAGGAGTCGAGGGACCCAAGGGAGACCCTGGCCCCCAAGGCCCAAGGGGACCCGTTGG CCCCCCTGGACGAGATGGCATCCCTGGACAGCCTGGACTTCCTGGTCCTCCTGGTCCCCCTGGGCCCCCC GGACCCCCTGGCCTTGGAGGAAACTTTGCTTCCCAGATGTCCTATGGCTATGATGAAAAATCAGCTGGAG TTTCCGTGCCTGGCCCCATGGGTCCTTCTGGTCCTCGTGGTCTCCCTGGCCCCCCTGGTGCACCTGGTCC ACAAGGTTTCCAAGGCCCCCCTGGTGAACCTGGCGAGCCTGGCGGTTCAGGTCCAATGGGTCCCCGAGGT CCCCCTGGCCCTCCTGGCAAGAATGGAGATGATGGGGAAGCTGGCAAGCCCGGCCGTCCTGGTGAGCGTG GACCTCCTGGACCTCAGGGTGCTCGTGGATTGCCTGGAACAGCTGGCCTCCCTGGAATGAAGGGACACCG AGGCTTCAGTGGTTTGGATGGTGCCAAAGGAGATGCTGGTCCTGCTGGTCCTAAGGGAGAGCCCGGCAGT CCTGGTGAAAACGGAGCTCCTGGCCAGATGGGTCCCCGAGGTCTGCCCGGTGAGAGAGGTCGCCCTGGAC CTCCTGGCACTGCTGGTGCTCGCGGTAACGATGGTGCTGTTGGTGCTGCTGGACCCCCTGGTCCCACCGG CCCCACTGGCCCTCCTGGCTTCCCTGGTGCAGTTGGTGCTAAGGGTGAAGCTGGTCCCCAAGGAGCTAGA GGCTCTGAAGGTCCCCAGGGTGTGCGTGGTGAGCCCGGACCCCCTGGCCCTGCTGGTGCTGCCGGCCCTG CTGGAAACCCTGGTGCTGATGGACAACCTGGCGCTAAAGGTGCCAATGGTGCTCCTGGTATTGCTGGTGC TCCTGGCTTCCCTGGTGCCCGAGGCCCCTCTGGACCCCAGGGCCCCAGCGGCCCTCCAGGTCCCAAGGGT AACAGTGGTGAACCTGGTGCTCCTGGCAACAAAGGAGACACTGGTGCCAAAGGAGAACCCGGTGCTACTG GAGTTCAAGGTCCCCCAGGCCCTGCCGGAGAAGAAGGAAAACGAGGAGCCCGTGGTGAGCCTGGACCTTC CGGACTGCCTGGACCTCCTGGCGAGCGTGGTGGACCTGGTAGCCGTGGTTTCCCTGGTGCTGATGGTGTT GCTGGCCCCAAGGGTCCTTCCGGTGAACGTGGTGCTCCCGGACCTGCTGGTCCCAAAGGTTCTCCTGGTG AAGCTGGTCGCCCCGGTGAAGCTGGTCTCCCTGGTGCCAAGGGTCTCACTGGCAGTCCTGGCAGCCCTGG TCCTGATGGCAAAACCGGCCCCCCTGGTCCCGCTGGTCAAGATGGTCGCCCTGGACCCGCAGGTCCTCCT GGAGCCCGTGGCCAGGCTGGTGTGATGGGATTCCCTGGACCTAAGGGTACCGCTGGAGAACCTGGAAAGG CTGGAGAGCGAGGCCTTCCCGGACCCCCTGGCGCTGTTGGTCCTGCTGGCAAAGATGGAGAAGCTGGAGC TCAGGGAGCCCCTGGCCCTGCTGGTCCTGCTGGTGAGAGAGGTGAACAAGGTCCCGCTGGCTCCCCTGGA TTCCAGGGTCTTCCTGGTCCTGCCGGTCCTCCTGGTGAAGCAGGCAAGCCTGGTGAACAGGGTGTTCCTG GAGACCTTGGTGCCCCTGGACCCTCTGGCGCAAGAGGCGAGAGAGGTTTCCCTGGTGAACGTGGTGTACA AGGTCCCCCAGGTCCTGCTGGTCCCCGAGGAAACAATGGTGCCCCCGGCAACGATGGTGCCAAGGGTGAT ACTGGTGCCCCCGGAGCTCCCGGTAGCCAGGGTGCCCCCGGTCTTCAGGGAATGCCTGGTGAACGTGGTG CAGCTGGTCTTCCAGGTCCTAAGGGTGACAGAGGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGG TAAAGATGGTGCCCGTGGTCTGACTGGTCCCATTGGTCCTCCTGGCCCTGCTGGTGCCCCTGGTGACAAG GGTGAAGCTGGTCCCAGTGGTCCTCCCGGTCCCACCGGAGCCCGTGGTGCTCCCGGAGACCGTGGTGAGG CTGGTCCCCCTGGTCCTGCTGGCTTTGCCGGCCCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGA ACCTGGTGATACTGGTGTTAAAGGTGATGCTGGTCCTCCTGGCCCTGCTGGTCCTGCTGGACCCCCCGGC CCCATTGGTAACGTTGGTGCTCCTGGACCCAAAGGTCCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTG GCTTCCCTGGTGCTGCTGGCCGTGTCGGTCCCCCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCC CGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTCCCCGTGGTGAGACTGGCCCTGCTGGACGTCCTGGTGAA GTTGGTCCCCCAGGTCCCCCCGGTCCTGCTGGTGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGCT CTCCTGGTACCCCTGGACCTCAGGGTATTGCTGGACAACGTGGTGTGGTCGGTCTTCCCGGTCAGAGAGG AGAAAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCTTCTGGATCAAGT GGTGAACGCGGTCCCCCTGGCCCCATGGGGCCCCCTGGATTGGCTGGTCCCCCTGGTGAATCTGGACGTG AGGGATCCCCTGGTGCTGAAGGCTCCCCTGGAAGGGATGGTGCTCCCGGGGCCAAGGGTGACCGTGGTGA GACTGGCCCCGCTGGCCCCCCTGGTGCCCCTGGTGCTCCCGGTGCTCCCGGCCCTGTTGGTCCCGCTGGC AAGAATGGCGATCGTGGTGAGACTGGTCCTGCTGGTCCTGCTGGTCCCATTGGCCCTGCTGGTGCCCGTG GCCCTGCTGGACCCCAAGGCCCCCGTGGTGACAAGGGTGAGACAGGCGAACAAGGTGACAGAGGCATAAA GGGTCATCGTGGCTTCTCTGGTCTCCAGGGTCCTCCTGGTTCTCCTGGTTCTCCTGGTGAACAAGGCCCC TCTGGAGCTTCAGGTCCTGCAGGCCCCCGGGGTCCCCCTGGCTCTGCTGGTTCTCCTGGCAAAGACGGAC TCAACGGTCTCCCTGGCCCCATTGGTCCCCCTGGTCCTCGAGGTCGCACTGGTGACAGCGGCCCTGCTGG TCCCCCCGGCCCTCCTGGACCCCCTGGCCCTCCTGGACCTCCCAGTGGCGGTTATGACTTCAGCTTCCTG CCTCAGCCACCTCAAGAGAAGTCTCAAGATGGTGGCCGCTACTACCGGGCCGATGATGCTAACGTGGTTC GTGACCGTGACCTTGAGGTGGACACCACCCTCAAGAGCCTGAGTCAGCAGATTGAGAACATCCGCAGCCC CGAAGGCAGCCGCAAGAACCCTGCCCGCACATGCCGCGACCTCAAGATGTGCCACTCTGACTGGAAGAGC GGAGAGTACTGGATCGACCCTAACCAAGGCTGCAACCTGGACGCCATCAAGGTCTACTGCAACATGGAGA CAGGTCAGACCTGTGTGTTCCCTACTCAGCCGTCTGTGCCTCAGAAGAACTGGTACATCAGCCCGAACCC CAAGGAAAAGAAGCACGTCTGGTTTGGAGAGAGCATGACCGATGGATTCCCGTTCGAGTACGGAAGCGAG GGCTCCGACCCCGCCGATGTCGCTATCCAGCTGACCTTCCTGCGCCTAATGTCCACCGAGGCCTCCCAGA ACATCACCTATCACTGCAAGAACAGCGTAGCCTACATGGACCAGCAGACTGGCAACCTCAAGAAGGCCCT GCTCCTCCAGGGATCCAACGAGATCGAGCTCAGAGGCGAAGGCAACAGTCGCTTCACCTACAGCACCCTT GTGGACGGCTGCACGAGTCACACCGGAACTTGGGGCAAGACAGTCATCGAATACAAAACCACCAAGACCT CCCGCCTGCCCATCATCGATGTGGCTCCCTTGGACATTGGTGCCCCAGACCAGGAATTCGGACTAGACAT TGGCCCTGCCTGCTTCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226667 representing NM_007742
Red=Cloning site Green=Tags(s) MFSFVDLRLLLLLGATALLTHGQEDIPEVSCIHNGLRVPNGETWKPEVCLICICHNGTAVCDDVQCNEEL DCPNPQRREGECCAFCPEEYVSPNSEDVGVEGPKGDPGPQGPRGPVGPPGRDGIPGQPGLPGPPGPPGPP GPPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGGSGPMGPRG PPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGS PGENGAPGQMGPRGLPGERGRPGPPGTAGARGNDGAVGAAGPPGPTGPTGPPGFPGAVGAKGEAGPQGAR GSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGPPGPKG NSGEPGAPGNKGDTGAKGEPGATGVQGPPGPAGEEGKRGARGEPGPSGLPGPPGERGGPGSRGFPGADGV AGPKGPSGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPAGPP GARGQAGVMGFPGPKGTAGEPGKAGERGLPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQGPAGSPG FQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGNNGAPGNDGAKGD TGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGARGLTGPIGPPGPAGAPGDK GEAGPSGPPGPTGARGAPGDRGEAGPPGPAGFAGPPGADGQPGAKGEPGDTGVKGDAGPPGPAGPAGPPG PIGNVGAPGPKGPRGAAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPVGKEGGKGPRGETGPAGRPGE VGPPGPPGPAGEKGSPGADGPAGSPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGSS GERGPPGPMGPPGLAGPPGESGREGSPGAEGSPGRDGAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAG KNGDRGETGPAGPAGPIGPAGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGSPGSPGEQGP SGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPGPPSGGYDFSFL PQPPQEKSQDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKS GEYWIDPNQGCNLDAIKVYCNMETGQTCVFPTQPSVPQKNWYISPNPKEKKHVWFGESMTDGFPFEYGSE GSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIELRGEGNSRFTYSTL VDGCTSHTGTWGKTVIEYKTTKTSRLPIIDVAPLDIGAPDQEFGLDIGPACFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007742 |
ORF Size | 4359 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_007742.2, NM_007742.3, NM_007742.4, NP_031768.2 |
RefSeq Size | 4709 |
RefSeq ORF | 4362 |
Locus ID | 12842 |
MW | 138.5 kDa |
Gene Summary | This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing. [provided by RefSeq, Nov 2015] |
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