Lck (NM_010693) Mouse Tagged ORF Clone

CAT#: MR226723

Lck (Myc-DDK-tagged) - Mouse lymphocyte protein tyrosine kinase (Lck), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lck
• Synonyms: Hck-3; Lsk; Lskt; p56; p56Lck
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226723 representing NM_010693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGTGTCTGCAGCTCAAACCCTGAAGATGACTGGATGGAGAACATTGACGTGTGTGAAAACTGCC
  ACTATCCCATAGTCCCGCTGGACAGCAAGATCTCGCTGCCCATCCGGAATGGCTCTGAAGTGCGGGACCC
  ACTGGTCACCTATGAGGGATCTCTCCCACCAGCATCCCCGCTGCAAGACAACCTGGTTATCGCCCTGCAC
  AGTTATGAGCCCTCCCATGATGGAGACTTGGGCTTTGAGAAGGGTGAACAGCTCCGAATCCTGGAGCAGA
  GCGGTGAGTGGTGGAAGGCTCAGTCCCTGACGACTGGCCAAGAAGGCTTCATTCCCTTCAACTTCGTGGC
  GAAAGCAAACAGCCTGGAGCCTGAACCTTGGTTCTTCAAGAATCTGAGCCGTAAGGACGCCGAGCGGCAG
  CTTTTGGCGCCCGGGAACACGCATGGATCCTTCCTGATCCGGGAAAGCGAAAGCACTGCGGGGTCCTTTT
  CCCTGTCGGTCAGAGACTTCGACCAGAACCAGGGAGAAGTGGTGAAACATTACAAGATCCGTAACCTAGA
  CAACGGTGGCTTCTACATCTCCCCTCGTATCACTTTTCCCGGATTGCACGATCTAGTCCGCCATTACACC
  AACGCCTCTGATGGGCTGTGCACAAAGTTGAGCCGTCCTTGCCAGACTCAGAAGCCCCAGAAACCATGGT
  GGGAGGACGAATGGGAAGTTCCCAGGGAAACACTGAAGTTGGTGGAGCGGCTGGGAGCTGGCCAGTTCGG
  GGAAGTGTGGATGGGGTACTACAACGGACACACGAAGGTGGCGGTGAAGAGTCTGAAACAAGGGAGCATG
  TCCCCCGACGCCTTCCTGGCTGAGGCTAACCTCATGAAGCAGCTGCAGCACCCGCGGCTAGTCCGGCTTT
  ATGCAGTGGTCACCCAGGAACCCATCTACATCATCACGGAATACATGGAGAACGGGAGCCTAGTAGATTT
  TCTCAAGACTCCCTCGGGCATCAAGTTGAATGTCAACAAACTTTTGGACATGGCAGCCCAGATTGCAGAG
  GGCATGGCGTTCATCGAAGAACAGAATTACATACATCGGGACCTGCGCGCCGCCAACATCCTGGTGTCTG
  ACACGCTGAGCTGCAAGATTGCAGACTTTGGCCTGGCGCGCCTCATTGAGGACAATGAGTACACGGCCCG
  GGAGGGGGCCAAATTTCCCATTAAGTGGACAGCACCAGAAGCCATTAACTATGGGACCTTCACCATCAAG
  TCAGACGTGTGGTCCTTCGGGATCTTGCTTACAGAGATTGTCACCCACGGTCGAATCCCTTACCCAGGAA
  TGACCAACCCTGAAGTCATTCAGAACCTGGAGAGAGGCTACCGCATGGTGAGACCTGACAACTGTCCGGA
  AGAGCTGTACCACCTCATGATGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCACGTTTGACTACCTT
  CGGAGTGTTCTGGATGACTTCTTCACAGCCACAGAGGGCCAGTACCAGCCCCAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226723 representing NM_010693
  Red=Cloning site Green=Tags(s)
  
  MGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALH
  SYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQ
  LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYT
  NASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSM
  SPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAE
  GMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIK
  SDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYL
  RSVLDDFFTATEGQYQPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010693
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010693.1, NM_010693.2, NM_010693.3, NP_034823.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 1530 bp
• Locus ID: 16818
• MW: 57.9 kDa