Csf1 (NM_007778) Mouse Tagged ORF Clone

CAT#: MR226827

Csf1 (Myc-DDK-tagged) - Mouse colony stimulating factor 1 (macrophage) (Csf1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csf1
• Synonyms: C87615; Csfm; MCSF; op
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226827 representing NM_007778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCGCGGGGCGCCGCGGGGCGCTGCCCTTCTTCGACATGGCTGGGCTCCCGGCTGCTGCTGGTCT
  GTCTCCTCATGAGCAGGAGTATTGCCAAGGAGGTGTCAGAACACTGTAGCCACATGATTGGGAATGGACA
  CCTGAAGGTCCTGCAGCAGTTGATCGACAGTCAAATGGAGACTTCATGCCAGATTGCCTTTGAATTTGTA
  GACCAGGAACAGCTGGATGATCCTGTTTGCTACCTAAAGAAGGCCTTTTTTCTGGTACAAGACATAATAG
  ATGAGACCATGCGCTTTAAAGACAACACCCCCAATGCTAACGCCACCGAGAGGCTCCAGGAACTCTCCAA
  TAACCTGAACAGCTGCTTCACCAAGGACTATGAGGAGCAGAACAAGGCCTGTGTCCGAACTTTCCATGAG
  ACTCCTCTCCAGCTGCTGGAGAAGATCAAGAACTTCTTTAATGAAACAAAGAATCTCCTTGAAAAGGACT
  GGAACATTTTTACCAAGAACTGCAACAACAGCTTTGCTAAGTGCTCTAGCCGAGATGTGGTGACCAAGCC
  TGATTGCAACTGCCTGTACCCTAAAGCCACCCCTAGCAGTGACCCGGCCTCTGCCTCCCCTCACCAGCCC
  CCCGCCCCCTCCATGGCCCCTCTGGCTGGCTTGGCTTGGGATGATTCTCAGAGGACAGAGGGCAGCTCCC
  TCTTGCCCAGTGAGCTTCCCCTTCGCATAGAGGACCCAGGCAGTGCCAAGCAGCGACCACCCAGGAGTAC
  CTGCCAGACCCTCGAGTCAACAGAGCAACCAAACCATGGGGACAGACTCACTGAGGACTCACAACCTCAT
  CCTTCTGCGGGGGGGCCCGTCCCTGGGGTGGAAGACATTCTTGAATCTTCACTGGGCACTAACTGGGTCC
  TAGAAGAAGCTTCTGGAGAGGCTAGTGAGGGATTTTTGACCCAGGAAGCAAAGTTTTCCCCCTCCACGCC
  TGTAGGGGGCAGCATCCAGGCAGAGACTGACAGACCCAGGGCCCTCTCAGCATCTCCATTCCCTAAATCA
  ACAGAGGACCAAAAGCCAGTGGATATAACAGACAGGCCGTTGACAGAGGTGAACCCTATGAGACCCATTG
  GCCAGACACAGAATAATACTCCTGAGAAGACTGATGGTACATCCACGCTGCGTGAAGACCACCAGGAGCC
  AGGCTCTCCCCATATTGCGACACCGAATCCCCAACGAGTCAGCAACTCAGCCACCCCCGTTGCTCAGTTA
  CTGCTTCCCAAAAGCCACTCTTGGGGCATTGTGCTGCCCCTTGGGGAGCTTGAGGGCAAGAGAAGTACCA
  GGGATCGAAGGAGCCCCGCAGAGCTGGAAGGAGGATCAGCAAGTGAGGGGGCAGCCAGGCCTGTGGCCCG
  TTTTAATTCCATTCCTTTGACTGACACAGGCCATGTGGAGCAGCATGAGGGATCCTCTGACCCCCAGATC
  CCTGAGTCTGTCTTCCACCTGCTGGTGCCGGGCATCATCCTAGTCTTGCTGACTGTTGGGGGCCTCCTGT
  TCTACAAGTGGAAGTGGAGGAGCCATCGAGACCCTCAGACATTGGATTCTTCTGTGGGGCGACCAGAGGA
  CAGCTCCCTGACCCAGGATGAGGACAGACAGGTGGAACTGCCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226827 representing NM_007778
  Red=Cloning site Green=Tags(s)
  
  MTARGAAGRCPSSTWLGSRLLLVCLLMSRSIAKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFV
  DQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHE
  TPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKPDCNCLYPKATPSSDPASASPHQP
  PAPSMAPLAGLAWDDSQRTEGSSLLPSELPLRIEDPGSAKQRPPRSTCQTLESTEQPNHGDRLTEDSQPH
  PSAGGPVPGVEDILESSLGTNWVLEEASGEASEGFLTQEAKFSPSTPVGGSIQAETDRPRALSASPFPKS
  TEDQKPVDITDRPLTEVNPMRPIGQTQNNTPEKTDGTSTLREDHQEPGSPHIATPNPQRVSNSATPVAQL
  LLPKSHSWGIVLPLGELEGKRSTRDRRSPAELEGGSASEGAARPVARFNSIPLTDTGHVEQHEGSSDPQI
  PESVFHLLVPGIILVLLTVGGLLFYKWKWRSHRDPQTLDSSVGRPEDSSLTQDEDRQVELPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007778
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007778.1, NM_007778.2, NM_007778.3, NM_007778.4, NP_031804.3
• RefSeq Size: 4192
• RefSeq ORF: 1659
• Locus ID: 12977
• MW: 61.1 kDa
• Gene Summary: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [UniProtKB/Swiss-Prot Function]