Tap1 (NM_013683) Mouse Tagged ORF Clone

CAT#: MR226873

Tap1 (Myc-DDK-tagged) - Mouse transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) (Tap1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tap1
• Synonyms: ABC17; Abcb2; APT1; Ham-1; Ham1; MTP1; PSF1; RING4; TAP; Tap-1; Y3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226873 representing NM_013683
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCACGTCTGGCTGGCGGCCGCCCTGCTCCTTCTGGTGGACTGGCTGCTGCTGCGGCCCATGC
  TCCCGGGAATCTTCTCCCTGTTGGTTCCCGAGGTGCCGCTGCTCCGGGTCTGGGTGGTGGGCCTGAGTCG
  CTGGGCCATCCTAGGACTAGGGGTCCGCGGGGTCCTCGGGGTCACCGCAGGAGCCCATGGCTGGCTGGCT
  GCTTTGCAGCCGCTGGTGGCCGCACTGAGTTTGGCCCTGCCTGGACTTGCCTTGTTCCGAGAGCTGGCCG
  CCTGGGGAACACTCCGGGAGGGTGACAGCGCTGGATTACTGTACTGGAACAGTCGTCCAGATGCCTTCGC
  TATCAGTTATGTGGCAGCATTGCCCGCAGCCGCCCTGTGGCACAAGTTGGGGAGCCTCTGGGCGCCCAGC
  GGCAACAGGGACGCTGGAGACATGCTGTGTCGGATGCTGGGCTTCCTGGGCCCTAAGAAGAGACGTCTCT
  ACCTGGTTCTGGTTCTCTTGATTCTCTCTTGCCTTGGGGAAATGGCCATTCCCTTCTTCACGGGCCGCAT
  CACTGACTGGATTCTTCAGGATAAGACAGTTCCTAGCTTCACCCGCAACATATGGCTCATGTCCATTCTC
  ACCATAGCCAGCACAGCGCTGGAGTTTGCAAGTGATGGAATCTACAACATCACCATGGGACACATGCACG
  GCCGTGTGCACAGAGAGGTGTTTCGGGCCGTCCTTCGCCAGGAGACAGGGTTTTTCCTGAAGAACCCAGC
  AGGTTCCATCACATCTCGGGTGACTGAGGACACAGCCAACGTGTGCGAGTCCATTAGTGGCACGCTGAGC
  CTGCTGCTGTGGTACCTGGGGCGAGCCCTGTGTCTCTTGGTGTTCATGTTTTGGGGGTCACCGTACCTCA
  CTCTGGTCACCCTGATCAACCTGCCCCTGCTTTTTCTTTTGCCTAAGAAGCTGGGAAAAGTGCATCAGTC
  ACTGGCAGTGAAGGTGCAGGAGTCTCTAGCAAAGTCCACGCAGGTGGCCCTTGAGGCCTTATCGGCGATG
  CCTACTGTGCGGAGCTTTGCCAACGAGGAGGGTGAGGCCCAGAAGTTCAGGCAGAAGTTGGAAGAAATGA
  AGACTCTAAACAAGAAGGAGGCCTTGGCTTACGTGGCTGAAGTCTGGACCACGAGTGTCTCGGGAATGCT
  GCTGAAGGTGGGAATTCTGTACCTGGGCGGGCAGCTGGTGATCAGAGGGGCTGTCAGCAGCGGCAACCTT
  GTCTCATTCGTTCTCTACCAGCTTCAGTTCACCCAGGCTGTTCAGGTCCTGCTCTCCCTCTACCCCTCCA
  TGCAGAAGGCTGTGGGCTCCTCAGAGAAAATATTCGAATACTTGGACCGGACTCCTTGCTCTCCACTCAG
  TGGCTCGTTGGCACCCTCAAACATGAAAGGCCTTGTGGAGTTCCAAGATGTCTCTTTTGCCTACCCAAAC
  CAGCCCAAAGTCCAGGTGCTTCAGGGGCTGACGTTCACCCTGCATCCTGGAACGGTGACAGCGTTGGTGG
  GACCCAATGGATCAGGGAAGAGCACCGTGGCTGCCCTGCTGCAGAACCTGTACCAGCCCACCGGGGGCCA
  GCTGCTGCTGGATGGCCAGTGCCTGGTCCAGTATGATCACCATTACCTGCACACTCAGGTGGCCGCAGTG
  GGACAAGAGCCGCTGCTATTTGGAAGAAGCTTTCGAGAAAATATTGCGTATGGCCTGAACCGGACTCCAA
  CCATGGAGGAAATCACAGCTGTGGCCGTGGAGTCTGGAGCCCACGATTTCATCTCTGGGTTCCCTCAGGG
  CTATGACACAGAGGTAGGTGAGACTGGGAACCAGCTGTCAGGAGGTCAGCGACAGGCAGTGGCCTTGGCC
  CGAGCCTTGATCCGGAAGCCACTCCTGCTTATCTTGGATGATGCCACCAGTGCCCTGGATGCTGGCAACC
  AGCTACGGGTCCAGCGGCTCCTGTATGAGAGCCCCAAGCGGGCTTCTCGGACGGTTCTTCTTATCACCCA
  GCAGCTCAGCCTGGCAGAGCAGGCCCACCACATCCTCTTTCTCAGAGAAGGCTCTGTCGGCGAGCAGGGC
  ACCCACCTGCAGCTCATGAAGAGAGGAGGGTGCTACCGGGCCATGGTAGAGGCTCTTGCGGCTCCTGCAG
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226873 representing NM_013683
  Red=Cloning site Green=Tags(s)
  
  MAAHVWLAAALLLLVDWLLLRPMLPGIFSLLVPEVPLLRVWVVGLSRWAILGLGVRGVLGVTAGAHGWLA
  ALQPLVAALSLALPGLALFRELAAWGTLREGDSAGLLYWNSRPDAFAISYVAALPAAALWHKLGSLWAPS
  GNRDAGDMLCRMLGFLGPKKRRLYLVLVLLILSCLGEMAIPFFTGRITDWILQDKTVPSFTRNIWLMSIL
  TIASTALEFASDGIYNITMGHMHGRVHREVFRAVLRQETGFFLKNPAGSITSRVTEDTANVCESISGTLS
  LLLWYLGRALCLLVFMFWGSPYLTLVTLINLPLLFLLPKKLGKVHQSLAVKVQESLAKSTQVALEALSAM
  PTVRSFANEEGEAQKFRQKLEEMKTLNKKEALAYVAEVWTTSVSGMLLKVGILYLGGQLVIRGAVSSGNL
  VSFVLYQLQFTQAVQVLLSLYPSMQKAVGSSEKIFEYLDRTPCSPLSGSLAPSNMKGLVEFQDVSFAYPN
  QPKVQVLQGLTFTLHPGTVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGQCLVQYDHHYLHTQVAAV
  GQEPLLFGRSFRENIAYGLNRTPTMEEITAVAVESGAHDFISGFPQGYDTEVGETGNQLSGGQRQAVALA
  RALIRKPLLLILDDATSALDAGNQLRVQRLLYESPKRASRTVLLITQQLSLAEQAHHILFLREGSVGEQG
  THLQLMKRGGCYRAMVEALAAPAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013683
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013683.1, NM_013683.2, NP_038711.2
• RefSeq Size: 2950
• RefSeq ORF: 2175
• Locus ID: 21354
• MW: 79.3 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]