Vip (NM_011702) Mouse Tagged ORF Clone

CAT#: MR226907

Vip (Myc-DDK-tagged) - Mouse vasoactive intestinal polypeptide (Vip)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vip
• Synonyms: MGC107202
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226907 representing NM_011702
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCAGAAGCAAGCCTCAGTTCCTGGCATTCCTGATACTCTTCAGTGTGCTGTTCTCTCAGTCGC
  TGGCCTGGCCTCTCTTTGGACCACCTTCTGTAGTGAGTAGGCTGGATGACAGGATGCCGTTTGAAGGAGC
  AGGTGACCCTGACCAAGTCTCTTTAAAAGCAGACTCTGACATCTTGCAGAATCCCTTAGCAGAAAATGGC
  ACACCCTATTATGATGTGTCAAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGCGATTACAGCAGAC
  TTCTGGGTCAGATTTCTGCCAAAAAATACCTTGAGTCACTCATTGGCAAACGAATCAGCAGCAGCATCTC
  GGAAGATCCTGTGCCAATCAAACGACACTCTGATGCCGTCTTCACAGATAACTACACCCGCCTCAGAAAG
  CAAATGGCTGTGAAGAAATACCTGAACTCCATCCTGAATGGAAAGAGGAGCAGTGAGGGAGATTCTGCAG
  ACTTTCTTGAAGAGCTGGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226907 representing NM_011702
  Red=Cloning site Green=Tags(s)
  
  MEARSKPQFLAFLILFSVLFSQSLAWPLFGPPSVVSRLDDRMPFEGAGDPDQVSLKADSDILQNPLAENG
  TPYYDVSRNARHADGVFTSDYSRLLGQISAKKYLESLIGKRISSSISEDPVPIKRHSDAVFTDNYTRLRK
  QMAVKKYLNSILNGKRSSEGDSADFLEELEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011702
• ORF Size: 513 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011702.1, NM_011702.2, NM_011702.3, NP_035832.1
• RefSeq Size: 1527
• RefSeq ORF: 516
• Locus ID: 22353
• MW: 19.6 kDa
• Gene Summary: This gene encodes a neuropeptide of the glucagon/secretin superfamily with potent bronchodilator, immunomodulator and anti-inflammatory properties. The encoded protein is proteolytically processed to generate two structurally similar neuropeptides - vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). In the digestive tract, VIP stimulates relaxation of enteric smooth muscle, secretion of water and electrolytes, release of insulin and glucagon, and inhibition of gastric acid secretion. In the cardiovascular system, VIP causes coronary vasodilation and stimulates contractility in the heart. Mice lacking VIP exhibit airway hyperresponsiveness and airway inflammation. Male mice lacking VIP exhibit moderate pulmonary arterial hypertension resulting in increased mortality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]