Sirpa (NM_007547) Mouse Tagged ORF Clone

CAT#: MR226909

Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sirpa
• Synonyms: AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226909 representing NM_007547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCGGCCCGGCCCCTGGCCGCCTAGGGCCGCTGCTGCTCTGCCTGCTGCTCTCCGCGTCCT
  GTTTCTGTACAGGAGCCACGGGGAAGGAACTGAAGGTGACTCAGCCTGAGAAATCAGTGTCTGTTGCTGC
  TGGGGATTCGACCGTTCTGAACTGCACTTTGACCTCCTTGTTGCCGGTGGGACCCATTAGGTGGTACAGA
  GGAGTAGGGCCAAGCCGGCTGTTGATCTACAGTTTCGCAGGAGAATACGTTCCTCGAATTAGAAATGTTT
  CAGATACTACTAAGAGAAACAATATGGACTTTTCCATCCGTATCAGTAATGTCACCCCAGCAGATGCTGG
  CATCTACTACTGTGTGAAGTTCCAGAAAGGATCATCAGAGCCTGACACAGAAATACAATCTGGAGGGGGA
  ACAGAGGTCTATGTACTCGCCAAACCTTCTCCACCGGAGGTATCCGGCCCAGCAGACAGGGGCATACCTG
  ACCAGAAAGTGAACTTCACCTGCAAGTCTCATGGCTTCTCTCCCCGGAATATCACCCTGAAGTGGTTCAA
  AGATGGGCAAGAACTCCACCCCTTGGAGACCACCGTGAACCCTAGTGGAAAGAATGTCTCCTACAACATC
  TCCAGCACAGTCAGGGTGGTACTAAACTCCATGGATGTTAATTCTAAGGTCATCTGCGAGGTAGCCCACA
  TCACCTTGGATAGAAGCCCTCTTCGTGGGATTGCTAACCTGTCTAACTTCATCCGAGTTTCACCCACCGT
  GAAGGTCACCCAACAGTCCCCGACGTCAATGAACCAGGTGAACCTCACCTGCCGGGCTGAGAGGTTCTAC
  CCCGAGGATCTCCAGCTGATCTGGCTGGAGAATGGAAACGTATCACGGAATGACACGCCCAAGAATCTCA
  CAAAGAACACGGATGGGACCTATAATTACACAAGCTTGTTCCTGGTGAACTCATCTGCTCATAGAGAGGA
  CGTGGTGTTCACGTGCCAGGTGAAGCACGACCAACAGCCAGCGATCACCCGAAACCATACCGTGCTGGGA
  TTTGCCCACTCGAGTGATCAAGGGAGCATGCAAACCTTCCCTGATAATAATGCTACCCACAACTGGAATG
  TCTTCATCGGTGTGGGCGTGGCGTGTGCTTTGCTCGTAGTCCTGCTGATGGCTGCTCTCTACCTCCTCCG
  GATCAAACAGAAGAAAGCCAAGGGGTCAACATCTTCCACACGGTTGCACGAGCCCGAGAAGAACGCCAGG
  GAAATAACCCAGATCCAGGACACAAATGACATCAACGACATCACATACGCAGACCTGAATCTGCCCAAAG
  AGAAGAAGCCCGCACCCCGGGCCCCTGAGCCTAACAACCACACAGAATATGCAAGCATTGAGACAGGCAA
  AGTGCCTAGGCCAGAGGATACCCTCACCTATGCTGACCTGGACATGGTCCACCTCAGCCGGGCACAGCCA
  GCCCCCAAGCCTGAGCCATCTTTCTCAGAGTATGCTAGTGTCCAGGTCCAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226909 representing NM_007547
  Red=Cloning site Green=Tags(s)
  
  MEPAGPAPGRLGPLLLCLLLSASCFCTGATGKELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYR
  GVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGG
  TEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNI
  SSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFY
  PEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLG
  FAHSSDQGSMQTFPDNNATHNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAR
  EITQIQDTNDINDITYADLNLPKEKKPAPRAPEPNNHTEYASIETGKVPRPEDTLTYADLDMVHLSRAQP
  APKPEPSFSEYASVQVQRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007547
• ORF Size: 1527 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007547.2, NM_007547.3, NM_007547.4, NP_031573.2
• RefSeq Size: 4031
• RefSeq ORF: 1530
• Locus ID: 19261
• MW: 56.4 kDa