Atg9a (NM_001003917) Mouse Tagged ORF Clone

CAT#: MR226916

Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (Atg9a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg9a
• Synonyms: Apg9l1; Atg9; Atg9l1; AU019532
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226916 representing NM_001003917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG
  ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT
  CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC
  ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG
  CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT
  GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT
  GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT
  ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT
  TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT
  CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC
  TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG
  GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA
  GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA
  TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT
  GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG
  CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA
  CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA
  TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG
  TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC
  ACATCCACTACATGCCTGACCACTGGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCA
  GCTCTTCCAGTACAAGGCAGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACACCCCTCATTCTC
  ATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGTCGAGGTCGTGG
  GTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAGTGGCTGTCTGG
  AGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGCTCATGCACTTT
  GCCATCACCAATCCCGGCTGGCAGCCCCCTCGTGAGAGCACAGCTTTCCTGGGCTTCCTCAAGGAGCAGG
  TGCAGCGAGATGGAGCAGCTGCTGGCCTGGCCCAGGGTGGCCTGCTCCCCGAGAATGCCCTCTTCACATC
  CATCCAGTCCTTACAGTCTGAGTCCGAGCCACTGAGCCTTATTGCAAATGTGGTAGCAGGCTCATCCTGC
  CGAGGACCCTCACTGTCCAGAGACCTGCAGGGCTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTCGAT
  CCTTCTCCCCTCTGCAGCCTGGAGCGGCCCCTCAAGGCCGGGTTCCCAGTACCATGACAGGCTCTGGAGT
  GGATGCCAGGACAGCCAGCTCTGGGAGTAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCCGAA
  TACGCGTCCACCGAGATGAGCCTGCACGCCCTGTACATGCACCAGCTCCACAAGCAGCAGACCCAGGCTG
  AGCCCGAGCGGCATGTGTGGCACCGCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGG
  AGAGGGTGCCCGGGCCCCCCAACCCATCCCCCGCTCGGCCAGCTATCCCTGTGCTACACCCCGGCCTGGA
  GCACCTGAGACCACCGCCCTGCATGGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAG
  TGCCCCGTGGCCCCTCTCACTTCTCCAGGCTGCCCCTGGGAGGATGGGCAGAAGATGGCCAGCCAGCATC
  AAGGCACCCAGAGCCGGTGCCAGAGGAGGGCTCAGAGGATGAACTCCCCCCTCAAGTGCACAAGGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226916 representing NM_001003917
  Red=Cloning site Green=Tags(s)
  
  MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEMFEL
  MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG
  VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH
  RILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL
  ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL
  QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV
  CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLIL
  IFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDGKTELSLMHF
  AITNPGWQPPRESTAFLGFLKEQVQRDGAAAGLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSC
  RGPSLSRDLQGSRHRADVASALRSFSPLQPGAAPQGRVPSTMTGSGVDARTASSGSSVWEGQLQSLVLSE
  YASTEMSLHALYMHQLHKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPG
  APETTALHGGFQRRYGGITDPGTVPRGPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003917
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003917.2, NM_001003917.3, NM_001003917.4, NP_001003917.2
• RefSeq Size: 3764 bp
• RefSeq ORF: 2520 bp
• Locus ID: 245860
• Cytogenetics: 1 C4
• MW: 94.9 kDa