Trpa1 (NM_177781) Mouse Tagged ORF Clone

CAT#: MR227099

Trpa1 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily A, member 1 (Trpa1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trpa1
• Synonyms: Anktm1; TRPA1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227099 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCGGCTTGAGGAGGATTCTGCTCCCGGAGGAAAGGAAGGAGGTCCAGGGCGTTGTCTATCGCG
  GCGTCGGGGAAGACATGGACTGCTCCAAGGAATCCTTTAAGGTGGACATTGAAGGAGATATGTGTAGATT
  AGAAGACTTCATCAAGAACCGAAGAAAACTAAGCAAATATGAGGATGAAAATCTCTGTCCTCTGCATCAC
  GCAGCAGCAGAAGGTCAAGTTGAACTGATGGAACTGATCATCAATGGTTCTTCGTGTGAAGTGCTGAATA
  TAATGGATGGTTATGGAAATACCCCACTGCATTGTGCTGCAGAAAAAAATCAAGTTGAAAGTGTAAAGTT
  TCTTCTCAGCCAAGGAGCAAATCCAAACCTCCGAAATAGAAACATGATGTCACCCCTTCACATAGCTGTG
  CATGGCATGTACAACGAAGTGATCAAGGTGTTGACTGAGCACAAGGCCACTAACATCAATTTAGAAGGAG
  AGAATGGGAACACGGCTTTGATGTCCACGTGTGCCAAAGACAACAGTGAAGCTTTGCAAATTTTGTTAGA
  AAAAGGAGCTAAGCTGTGTAAATCAAATAAGTGGGGAGACTACCCTGTGCACCAGGCAGCATTTTCAGGT
  GCCAAAAAATGCATGGAATTAATCTTAGCATATGGTGAAAAGAACGGCTACAGCAGGGAGACTCACATTA
  ATTTTGTGAATCACAAGAAAGCCAGCCCTCTCCACCTAGCAGTTCAAAGCGGAGACTTGGACATGATTAA
  GATGTGCCTGGACAACGGTGCACACATCGACATGATGGAGAATGCCAAATGCATGGCCCTCCATTTTGCT
  GCAACCCAGGGAGCCACTGACATCGTTAAGCTCATGATCTCATCCTATACCGGAAGTAGTGATATTGTGA
  ATGCAGTTGATGGCAATCAGGAGACCCTGCTTCACAGAGCCTCGTTATTTGATCACCATGACCTGGCAGA
  ATACCTAATATCAGTGGGAGCAGACATCAACAGCACTGATTCTGAAGGACGCTCTCCACTTATTTTAGCA
  ACAGCTTCTGCATCCTGGAACATTGTGAATTTGCTCCTCTGTAAAGGTGCCAAAGTAGACATAAAAGATC
  ATCTTGGACGTAACTTTTTGCATTTGACTGTGCAGCAGCCTTATGGACTAAGAAATTTGCGGCCTGAGTT
  TATGCAGATGCAACACATCAAAGAGCTGGTGATGGATGAAGACAATGACGGATGCACACCTCTCCATTAT
  GCCTGTAGGCAGGGGGTTCCTGTCTCTGTAAATAACCTCCTTGGCTTCAATGTGTCCATTCATAGCAAAA
  GTAAAGATAAGAAGTCGCCCCTGCATTTTGCAGCCAGTTATGGGCGCATCAATACATGTCAGAGACTTCT
  GCAAGACATAAGTGATACGAGGCTTTTGAATGAAGGGGATCTCCATGGGATGACCCCTCTCCACCTGGCA
  GCAAAAAATGGGCATGATAAAGTCGTTCAACTCCTTCTGAAGAAAGGGGCCTTATTTCTCAGTGACCACA
  ATGGCTGGACTGCTTTGCATCACGCCTCCATGGGTGGGTACACTCAGACCATGAAGGTCATTCTTGATAC
  TAACTTGAAATGCACAGACCGACTAGATGAAGAAGGGAACACAGCACTCCACTTTGCAGCACGGGAAGGC
  CATGCCAAGGCTGTTGCAATGCTTTTGAGCTACAATGCTGACATCCTCCTGAACAAGAAGCAAGCTTCCT
  TTCTGCATATTGCCCTGCACAATAAGCGCAAGGAAGTGGTTCTCACAACCATCAGAAATAAAAGATGGGA
  TGAGTGTCTTCAAGTTTTCACTCATAATTCTCCAAGCAATCGATGTCCAATCATGGAGATGGTAGAATAC
  CTCCCCGAGTGCATGAAAGTTCTTTTAGATTTCTGCATGATACCTTCCACAGAAGACAAGTCCTGTCAAG
  ACTACCATATTGAGTATAATTTCAAGTATCTCCAATGCCCATTATCCATGACCAAAAAAGTAGCACCTAC
  CCAGGATGTGGTATATGAGCCTCTTACAATCCTCAATGTCATGGTCCAACATAACCGCATAGAACTCCTC
  AACCACCCTGTGTGTAGGGAGTACTTACTCATGAAATGGTGTGCCTATGGATTCAGAGCCCATATGATGA
  ACCTAGGATCTTATTGTCTTGGTCTCATACCCATGACCCTTCTTGTTGTCAAAATACAGCCTGGAATGGC
  CTTCAATTCTACTGGAATAATCAATGGAACTAGTAGTACTCATGAGGAAAGAATAGACACTCTGAATTCA
  TTTCCAATAAAAATATGTATGATTCTAGTTTTTTTATCAAGTATATTTGGATATTGCAAAGAAGTGATCC
  AAATTTTCCAACAGAAAAGGAATTACTTCCTGGATTACAACAATGCTCTGGAATGGGTTATCTATACAAC
  TAGTATCATCTTCGTGTTGCCCTTGTTCCTCAACATCCCAGCGTATATGCAGTGGCAATGTGGAGCAATA
  GCGATATTCTTCTACTGGATGAACTTCCTACTGTATCTTCAAAGGTTTGAGAACTGTGGAATTTTCATTG
  TTATGTTGGAGGTGATTTTTAAAACATTGCTGAGATCGACCGGAGTGTTTATCTTCCTCCTACTGGCTTT
  TGGCCTCAGCTTTTATGTTCTCCTGAATTTCCAAGATGCCTTCAGCACCCCATTGCTTTCCTTAATCCAG
  ACATTCAGTATGATGCTAGGAGACATCAATTATCGAGATGCCTTCCTAGAACCATTGTTTAGAAATGAGT
  TGGCATACCCAGTCCTGACCTTTGGGCAGCTTATTGCCTTCACAATGTTTGTCCCAATTGTTCTCATGAA
  CTTACTGATTGGCTTGGCGGTTGGGGACATTGCTGAGGTCCAGAAGCATGCGTCATTGAAGAGGATTGCT
  ATGCAGGTGGAACTTCATACCAACTTAGAAAAAAAGCTGCCACTCTGGTACTTACGCAAAGTGGATCAGA
  GGTCCACCATCGTGTATCCAAATAGACCCAGGCACGGCAGGATGCTACGGTTTTTTCATTACTTTCTTAA
  TATGCAAGAAACACGACAAGAAGTACCAAACATTGACACATGCTTGGAAATGGAAATATTGAAACAGAAA
  TATCGGCTGAAGGACCTCACTTCCCTCTTGGAAAAGCAGCATGAGCTCATCAAACTCATCATCCAGAAGA
  TGGAGATCATCTCAGAGACAGAAGATGAAGATAACCATTGCTCTTTCCAAGACAGGTTCAAGAAGGAGAG
  GCTGGAACAGATGCACAGCAAGTGGAATTTTGTCTTAAACGCAGTTAAGACTAAAACACATTGTTCTATT
  AGCCACCCGGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227099 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRGLRRILLPEERKEVQGVVYRGVGEDMDCSKESFKVDIEGDMCRLEDFIKNRRKLSKYEDENLCPLHH
  AAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAV
  HGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSG
  AKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFA
  ATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILA
  TASASWNIVNLLLCKGAKVDIKDHLGRNFLHLTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHY
  ACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLA
  AKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEEGNTALHFAAREG
  HAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEY
  LPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCPLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELL
  NHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIINGTSSTHEERIDTLNS
  FPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAI
  AIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQ
  TFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIA
  MQVELHTNLEKKLPLWYLRKVDQRSTIVYPNRPRHGRMLRFFHYFLNMQETRQEVPNIDTCLEMEILKQK
  YRLKDLTSLLEKQHELIKLIIQKMEIISETEDEDNHCSFQDRFKKERLEQMHSKWNFVLNAVKTKTHCSI
  SHPDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177781
• ORF Size: 3378 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177781.1, NM_177781.2, NM_177781.3, NM_177781.4, NM_177781.5, NP_808449.1
• RefSeq Size: 4263
• RefSeq ORF: 3378
• Locus ID: 277328
• MW: 128.5 kDa