Mnat1 (NM_008612) Mouse Tagged ORF Clone

CAT#: MR227103

Mnat1 (Myc-DDK-tagged) - Mouse menage a trois 1 (Mnat1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mnat1
• Synonyms: E130115E11Rik; MAT1; P36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227103 representing NM_008612
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCAGGGCTGCCCCAGGTGTAAGACCACCAAGTACCGGAACCCTTCTTTGAAGCTGATGGTGA
  ACGTGTGCGGACACACTCTGTGTGAGAGTTGTGTGGACTTACTGTTTGTAAGAGGAGCTGGAAACTGTCC
  TGAGTGTGGGACTCCACTGAGAAAGAGCAACTTCAGGGTACAACTCTTTGAAGATCCCACTGTTGATAAG
  GAGGTTGAAATTCGGAAAAAAGTACTAAAGATATACAATAAAAGGGAAGAAGATTTTCCTAGTTTAAGAG
  AGTATAATGATTTTCTGGAAGAAGTGGAGGAAATCGTTTTCAATTTGACCAACAATGTGGATTTGGAGAA
  CACTAAAAAGAAAATGGAAATATATCAAAAAGAAAACAAAGATGTCATTCAGAAAAACAAATTAAAGCTG
  ACGCGGGAGCAGGAGGAGTTGGAGGAAGCATTGGAGGTAGAACGCCAAGAACATGAGCAGCGACGGCTGT
  TCATACAAAAGGAAGAAGAGCTGCAGCAGGCTCTGAAAAGGAAGAACAAGCAGGCCTTTTTAGATGAGCT
  GGAGAGCTCCGACCTCCCTGTTGCTCTGCTCCTGGCGCAGCATAAAGACAGATCGACCCAGCTGGAAATG
  CAGCTCGAGAAGCCCAGATCCATGAAGCCAGTGACGTTTTCCACAGGAATTAAAATGGGTCAACAGATTT
  CATTAGCACCAATCCAAAAGCTGGAAGAAGCTCTTTATGAGTACCAGCCCCTGCAGATAGAGACCTGTGG
  CCCACAAGTTCCTGAGCAAGAGCTCTTAGGACGGCTTGGGTATTTAAACCACGTCCGGGCTGCATCTCCA
  CAGGATCTTGCTGGCGGCTATACTTCTTCTCTTGCTTGTCACAGAGCGCTACAGGATGCGTTCAGTGGGC
  TCTTCTGGCAGCCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227103 representing NM_008612
  Red=Cloning site Green=Tags(s)
  
  MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDK
  EVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLENTKKKMEIYQKENKDVIQKNKLKL
  TREQEELEEALEVERQEHEQRRLFIQKEEELQQALKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEM
  QLEKPRSMKPVTFSTGIKMGQQISLAPIQKLEEALYEYQPLQIETCGPQVPEQELLGRLGYLNHVRAASP
  QDLAGGYTSSLACHRALQDAFSGLFWQPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008612
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008612.1, NM_008612.2, NP_032638.2
• RefSeq Size: 2505
• RefSeq ORF: 930
• Locus ID: 17420
• MW: 36.3 kDa
• Gene Summary: Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. [UniProtKB/Swiss-Prot Function]