Kcnj3 (NM_008426) Mouse Tagged ORF Clone

CAT#: MR227141

Kcnj3 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 3 (Kcnj3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj3
• Synonyms: GIRK-1; GIRK1; Kcnf3; Kir3.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227141 representing NM_008426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCACTCCGAAGGAAATTTGGGGACGATTACCAGGTAGTGACCACTTCGTCGAGCGGTTCGGGCT
  TACAGCCTCAGGGGCCAGGACAGGGCCCGCAGCAGCAGCTGGTGCCCAAGAAGAAACGGCAGCGGTTCGT
  GGACAAGAACGGCCGGTGCAATGTGCAGCATGGTAACCTGGGCAGCGAGACCAGTCGCTACCTTTCGGAC
  CTCTTCACTACCCTGGTGGATCTCAAGTGGCGTTGGAACCTCTTTATCTTCATCCTCACCTACACCGTGG
  CCTGGCTCTTCATGGCGTCCATGTGGTGGGTGATCGCTTATACCCGGGGCGACCTGAACAAAGCCCATGT
  CGGCAACTACACTCCCTGTGTGGCCAATGTCTATAACTTCCCCTCTGCCTTCCTCTTCTTCATCGAGACC
  GAGGCCACCATCGGCTATGGCTACCGCTACATCACAGATAAGTGCCCCGAGGGCATCATCCTCTTCCTCT
  TCCAGTCCATCCTTGGCTCCATCGTGGACGCTTTCCTCATCGGCTGCATGTTCATCAAGATGTCCCAGCC
  CAAAAAGCGCGCCGAGACCCTCATGTTTAGCGAGCATGCGGTTATCTCCATGAGGGACGGAAAACTCACT
  CTCATGTTCCGGGTGGGCAACCTGCGCAACAGCCACATGGTCTCCGCGCAGATCCGCTGCAAGCTGCTCA
  AATCTCGGCAGACACCTGAGGGTGAGTTCCTTCCCCTTGACCAACTTGAACTGGATGTAGGTTTTAGTAC
  AGGGGCCGATCAACTTTTTCTTGTGTCCCCTCTCACAATTTGCCACGTGATCGATGCCAAAAGCCCCTTC
  TATGACCTATCCCAGCGAAGCATGCAAACTGAACAGTTCGAGGTTGTCGTCATCCTGGAAGGCATTGTGG
  AAACCACAGGAATGACTTGTCAAGCTCGAACATCATACACAGAGGACGAAGTGCTTTGGGGTCATCGTTT
  TTTCCCTGTAATTTCTTTAGAAGAAGGATTCTTTAAAGTCGATTACTCCCAGTTCCATGCAACCTTTGAA
  GTTCCAACCCCTCCTTACAGCGTGAAAGAGCAGGAGGAAATGCTTCTCATGTCCTCCCCTTTAATAGCAC
  CAGCCATAACCAACAGCAAAGAAAGACACAATTCTGTGGAGTGTTTAGATGGACTAGATGACATTAGCAC
  AAAACTTCCATCGAAGCTGCAGAAAATTACGGGGAGAGAAGACTTTCCCAAAAAACTTCTGAGAATGAGT
  TCTACAACCTCAGAAAAAGCCTATAGTTTGGGTGATTTGCCCATGAAACTCCAACGAATAAGTTCAGTTC
  CTGGCAACTCGGAAGAAAAACTGGTTTCTAAAACCACCAAGATGTTATCTGATCCCATGAGCCAGTCTGT
  GGCCGATTTGCCACCAAAGCTTCAAAAGATGGCTGGAGGACCTACCAGGATGGAAGGAAATCTTCCAGCC
  AAACTAAGAAAAATGAACTCTGACCGTTTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227141 representing NM_008426
  Red=Cloning site Green=Tags(s)
  
  MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQGPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSD
  LFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIET
  EATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLT
  LMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPF
  YDLSQRSMQTEQFEVVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFE
  VPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDISTKLPSKLQKITGREDFPKKLLRMS
  STTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGPTRMEGNLPA
  KLRKMNSDRFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008426
• ORF Size: 1503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008426.1, NM_008426.2, NP_032452.1
• RefSeq Size: 1679
• RefSeq ORF: 1506
• Locus ID: 16519
• MW: 57 kDa
• Gene Summary: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat. [UniProtKB/Swiss-Prot Function]