Wnt3a (NM_009522) Mouse Tagged ORF Clone

CAT#: MR227165

Wnt3a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt3a
• Synonyms: vt; Wnt-3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227165 representing NM_009522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTCTCGGATACCTCTTAGTGCTCTGCAGCCTGAAGCAGGCTCTGGGCAGCTACCCGATCTGGT
  GGTCCTTGGCTGTGGGACCCCAGTACTCCTCTCTGAGCACTCAGCCCATTCTCTGTGCCAGCATCCCAGG
  CCTGGTACCGAAGCAGCTGCGCTTCTGCAGGAACTACGTGGAGATCATGCCCAGCGTGGCTGAGGGTGTC
  AAAGCGGGCATCCAGGAGTGCCAGCACCAGTTCCGAGGCCGGCGTTGGAACTGCACCACCGTCAGCAACA
  GCCTGGCCATCTTTGGCCCTGTTCTGGACAAAGCCACCCGGGAGTCAGCCTTTGTCCATGCCATCGCCTC
  CGCTGGAGTAGCTTTCGCAGTGACACGCTCCTGTGCAGAGGGATCAGCTGCTATCTGTGGGTGCAGCAGC
  CGCCTCCAGGGCTCCCCAGGCGAGGGCTGGAAGTGGGGCGGCTGTAGTGAGGACATTGAATTTGGAGGAA
  TGGTCTCTCGGGAGTTTGCCGATGCCAGGGAGAACCGGCCGGATGCCCGCTCTGCCATGAACCGTCACAA
  CAATGAGGCTGGGCGCCAGGCCATCGCCAGTCACATGCACCTCAAGTGCAAATGCCACGGGCTATCTGGC
  AGCTGTGAAGTGAAGACCTGCTGGTGGTCGCAGCCGGACTTCCGCACCATCGGGGATTTCCTCAAGGACA
  AGTATGACAGTGCCTCGGAGATGGTGGTAGAGAAACACCGAGAGTCTCGTGGCTGGGTGGAGACCCTGAG
  GCCACGTTACACGTACTTCAAGGTGCCGACAGAACGCGACCTGGTCTACTACGAGGCCTCACCCAACTTC
  TGCGAACCTAACCCCGAAACCGGCTCCTTCGGGACGCGTGACCGCACCTGCAATGTGAGCTCGCATGGCA
  TAGATGGGTGCGACCTGTTGTGCTGCGGGCGCGGGCATAACGCGCGCACTGAGCGACGGAGGGAGAAATG
  CCACTGTGTTTTCCATTGGTGCTGCTACGTCAGCTGCCAGGAGTGCACACGTGTCTATGACGTGCACACC
  TGCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227165 representing NM_009522
  Red=Cloning site Green=Tags(s)
  
  MAPLGYLLVLCSLKQALGSYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGV
  KAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSS
  RLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSG
  SCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNF
  CEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHT
  CK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009522
• ORF Size: 1056 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009522.1, NM_009522.2, NP_033548.1
• RefSeq Size: 2791
• RefSeq ORF: 1059
• Locus ID: 22416
• MW: 39.7 kDa
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. [UniProtKB/Swiss-Prot Function]