Rb1 (NM_009029) Mouse Tagged ORF Clone

CAT#: MR227287

Rb1 (Myc-DDK-tagged) - Mouse retinoblastoma 1 (Rb1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rb1
• Synonyms: pp105; pRb; Rb; Rb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227287 representing NM_009029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCAAAGCCCCGCGCAGAGCCGCGGCCGCCGAGCCCCCGCCACCGCCGCCGCCGCCGCCTCGGG
  AGGACGACCCCGCGCAGGACAGCGGCCCCGAAGAGCTGCCCCTGGCCAGGCTTGAGTTTGAAGAAATTGA
  AGAACCCGAATTTATTGCATTATGTCAAAAGTTAAAGGTACCCGATCATGTCAGAGAAAGAGCTTGGCTA
  ACTTGGGAGAAAGTTTCATCCGTGGATGGAATCCTGGAAGGATATATTCAAAAGAAGAAGGAACTCTGGG
  GCATCTGCATCTTTATCGCAGCAGTTGATCTAGATGAGATGCCATTCACTTTTACTGAGCTACAGAAAAG
  CATAGAAACCAGTGTCTATAAATTCTTTGACTTATTAAAAGAAATCGATACCAGTACCAAGGTTGATAAT
  GCTATGTCAAGACTATTGAAGAAGTATAATGTGTTATGTGCACTCTACAGCAAATTAGAACGGACGTGTG
  AACTTATATATTTGACACAACCCAGCAGTGCGTTATCTACTGAAATAAATTCTATGTTGGTGCTAAAAAT
  TTCTTGGATCACTTTTTTACTAGCTAAAGGAGAAGTATTACAGATGGAAGATGACCTGGTAATCTCATTT
  CAGCTAATGTTGTGTGTAGTTGACTATTTTATTAAGTTCTCACCTCCTGCACTACTCAGAGAGCCATACA
  AAACAGCTGCAATCCCCATTAATGGTTCACCTCGAACACCCAGAAGAGGTCAGAACAGGAGCGCTCGGAT
  AGCAAAACAACTAGAAAATGATACGAGGATTATCGAGGTTCTCTGTAAAGAACACGAGTGTAATATAGAT
  GAGGTGAAAAATGTTTATTTCAAAAATTTTATCCCTTTTATAAATTCACTTGGAATTGTATCATCTAATG
  GACTTCCAGAGGTTGAAAGTCTTTCTAAACGCTATGAAGAAGTTTATCTTAAAAACAAAGATTTAGATGC
  AAGACTGTTTTTGGATCATGATAAAACACTTCAGACTGATCCTATAGACAGTTTTGAAACAGAGAGAACG
  CCACGAAAAAACAACCCTGATGAAGAGGCAAACGTGGTTACTCCACACACTCCAGTTAGGACTGTTATGA
  ATACTATTCAACAATTAATGGTGATTTTAAATTCTGCAAGTGATCAGCCATCAGAAAATCTGATTTCCTA
  CTTCAATAATTGCACAGTGAATCCAAAAGAAAATATCCTAAAGAGAGTAAAGGATGTTGGGCACATCTTT
  AAAGAGAAGTTTGCTAACGCTGTGGGCCAGGGCTGTGTTGACATCGGAGTACAGCGATATAAACTTGGAG
  TCCGATTGTATTACCGTGTGATGGAATCCATGCTTAAATCAGAAGAAGAACGTTTGTCCATTCAGAATTT
  TAGCAAACTCCTAAATGACAACATCTTTCATATGTCTTTACTGGCCTGTGCTCTTGAAGTTGTAATGGCT
  ACGTATAGCAGAAGTACATTGCAGCATCTTGATTCTGGAACAGATTTGTCCTTCCCGTGGATTCTGAACG
  TACTTAATTTAAAAGCCTTTGATTTTTACAAAGTGATTGAAAGTTTTATCAAAGTGGAAGCCAACTTGAC
  AAGAGAAATGATAAAACATTTAGAAAGATGTGAGCATCGAATCATGGAATCCCTTGCATGGCTTTCAGAT
  TCACCTTTATTTGATCTCATTAAGCAGTCCAAGGATGGAGAAGGACCTGATAACCTTGAACCTGCTTGTC
  CTCTCAGCCTGCCTCTCCAGGGTAACCATACTGCAGCAGATATGTATCTTTCTCCTCTAAGATCTCCAAA
  GAAAAGAACTTCCACTACACGTGTAAATTCTGCTGCAAATACAGAGACACAAGCAGCCTCAGCCTTCCAT
  ACTCAGAAGCCATTGAAATCTACCTCCCTTGCCCTGTTTTACAAAAAAGTGTACCGTCTAGCATATCTCC
  GACTAAATACACTCTGTGCACGCCTTCTGTCTGACCACCCAGAGCTAGAGCACATCATCTGGACTCTGTT
  TCAGCATACATTGCAAAATGAGTATGAGCTCATGAGAGACCGACATTTGGACCAGATTATGATGTGCTCT
  ATGTATGGCATCTGCAAGGTGAAGAACATCGACCTCAAGTTCAAAATCATCGTCACTGCCTACAAGGATC
  TTCCTCACGCTGCCCAGGAGACCTTTAAACGTGTTTTGATCAGAGAAGAGGAGTTTGATTCCATTATAGT
  ATTCTATAACTCCGTTTTCATGCAGAGACTAAAAACAAATATTTTACAGTATGCCTCCACCAGGCCTCCT
  ACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTTCTAGTTCACCCTTACGGATTCCTG
  GAGGTAACATCTATATATCACCCCTAAAGAGTCCTTATAAAATTTCAGAAGGTCTGCCAACACCCACAAA
  AATGACTCCGAGATCAAGAATCTTGGTCTCAATTGGTGAATCATTTGGGACATCTGAAAAGTTCCAGAAA
  ATAAACCAGATGGTGTGTAATAGTGACAGAGTGCTCAAAAGAAGTGCTGAAGGCGGCAACCCCCCCAAAC
  CACTGAAAAAGCTGCGCTTTGACATCGAGGGAGCCGATGAAGCAGATGGGAGTAAACATCTCCCAGCGGA
  GTCCAAATTCCAACAGAAACTGGCAGAAATGACTTCCACTCGAACACGAATGCAAAAGCAGAGAATGAAT
  GAGAGCAAGGATGTCTCAAACAAGGAGGAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227287 representing NM_009029
  Red=Cloning site Green=Tags(s)
  
  MPPKAPRRAAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIALCQKLKVPDHVRERAWL
  TWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDN
  AMSRLLKKYNVLCALYSKLERTCELIYLTQPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISF
  QLMLCVVDYFIKFSPPALLREPYKTAAIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNID
  EVKNVYFKNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFETERT
  PRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKENILKRVKDVGHIF
  KEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMA
  TYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSD
  SPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFH
  TQKPLKSTSLALFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCS
  MYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPP
  TLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQK
  INQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGADEADGSKHLPAESKFQQKLAEMTSTRTRMQKQRMN
  ESKDVSNKEEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009029
• ORF Size: 2763 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009029.1, NM_009029.2, NP_033055.2
• RefSeq Size: 4642
• RefSeq ORF: 2766
• Locus ID: 19645
• MW: 105.8 kDa